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Systematically investigating the fluorescent signal readout of CRISPR-Cas12a for highly sensitive SARS-CoV-2 detection

The CRISPR/Cas system is widely used for molecular diagnostics after the discovery of trans-cleavage activity, especially now with the COVID-19 outbreak. However, the majority of contemporary trans-cleavage activity-based CRISPR/Cas biosensors exploited standard single-strand DNA (ssDNA) reporters,...

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Detalles Bibliográficos
Autores principales:	Liu, Sitong, Xie, Tie, Huang, Zhaohe, Pei, Xiaojing, Li, Shujing, He, Yifan, Tong, Yigang, Liu, Guoqi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527496/ https://www.ncbi.nlm.nih.gov/pubmed/36212739 http://dx.doi.org/10.1016/j.snb.2022.132746

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