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Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction

The highly organized transverse tubule (t-tubule) network facilitates cardiac excitation–contraction coupling and synchronous cardiac myocyte contraction. In cardiac failure secondary to myocardial infarction (MI), changes in the structure and organization of t-tubules result in impaired cardiac con...

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Autores principales: Perera, Tharushi, Pius, Charlene, Niort, Barbara, Radcliffe, Emma J., Dibb, Katharine M., Trafford, Andrew W., Pinali, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527908/
https://www.ncbi.nlm.nih.gov/pubmed/36189812
http://dx.doi.org/10.1098/rstb.2021.0331
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author Perera, Tharushi
Pius, Charlene
Niort, Barbara
Radcliffe, Emma J.
Dibb, Katharine M.
Trafford, Andrew W.
Pinali, Christian
author_facet Perera, Tharushi
Pius, Charlene
Niort, Barbara
Radcliffe, Emma J.
Dibb, Katharine M.
Trafford, Andrew W.
Pinali, Christian
author_sort Perera, Tharushi
collection PubMed
description The highly organized transverse tubule (t-tubule) network facilitates cardiac excitation–contraction coupling and synchronous cardiac myocyte contraction. In cardiac failure secondary to myocardial infarction (MI), changes in the structure and organization of t-tubules result in impaired cardiac contractility. However, there is still little knowledge on the regional variation of t-tubule remodelling in cardiac failure post-MI. Here, we investigate post-MI t-tubule remodelling in infarct border and remote regions, using serial block face scanning electron microscopy (SBF-SEM) applied to a translationally relevant sheep ischaemia reperfusion MI model and matched controls. We performed minimally invasive coronary angioplasty of the left anterior descending artery, followed by reperfusion after 90 min to establish the MI model. Left ventricular tissues obtained from control and MI hearts eight weeks post-MI were imaged using SBF-SEM. Image analysis generated three-dimensional reconstructions of the t-tubular network in control, MI border and remote regions. Quantitative analysis revealed that the MI border region was characterized by t-tubule depletion and fragmentation, dilation of surviving t-tubules and t-tubule elongation. This study highlights region-dependent remodelling of the tubular network post-MI and may provide novel localized therapeutic targets aimed at preservation or restoration of the t-tubules to manage cardiac contractility post-MI. This article is part of the theme issue ‘The cardiomyocyte: new revelations on the interplay between architecture and function in growth, health, and disease’.
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spelling pubmed-95279082022-10-14 Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction Perera, Tharushi Pius, Charlene Niort, Barbara Radcliffe, Emma J. Dibb, Katharine M. Trafford, Andrew W. Pinali, Christian Philos Trans R Soc Lond B Biol Sci Articles The highly organized transverse tubule (t-tubule) network facilitates cardiac excitation–contraction coupling and synchronous cardiac myocyte contraction. In cardiac failure secondary to myocardial infarction (MI), changes in the structure and organization of t-tubules result in impaired cardiac contractility. However, there is still little knowledge on the regional variation of t-tubule remodelling in cardiac failure post-MI. Here, we investigate post-MI t-tubule remodelling in infarct border and remote regions, using serial block face scanning electron microscopy (SBF-SEM) applied to a translationally relevant sheep ischaemia reperfusion MI model and matched controls. We performed minimally invasive coronary angioplasty of the left anterior descending artery, followed by reperfusion after 90 min to establish the MI model. Left ventricular tissues obtained from control and MI hearts eight weeks post-MI were imaged using SBF-SEM. Image analysis generated three-dimensional reconstructions of the t-tubular network in control, MI border and remote regions. Quantitative analysis revealed that the MI border region was characterized by t-tubule depletion and fragmentation, dilation of surviving t-tubules and t-tubule elongation. This study highlights region-dependent remodelling of the tubular network post-MI and may provide novel localized therapeutic targets aimed at preservation or restoration of the t-tubules to manage cardiac contractility post-MI. This article is part of the theme issue ‘The cardiomyocyte: new revelations on the interplay between architecture and function in growth, health, and disease’. The Royal Society 2022-11-21 2022-10-03 /pmc/articles/PMC9527908/ /pubmed/36189812 http://dx.doi.org/10.1098/rstb.2021.0331 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Articles
Perera, Tharushi
Pius, Charlene
Niort, Barbara
Radcliffe, Emma J.
Dibb, Katharine M.
Trafford, Andrew W.
Pinali, Christian
Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction
title Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction
title_full Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction
title_fullStr Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction
title_full_unstemmed Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction
title_short Serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction
title_sort serial block face scanning electron microscopy reveals region-dependent remodelling of transverse tubules post-myocardial infarction
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527908/
https://www.ncbi.nlm.nih.gov/pubmed/36189812
http://dx.doi.org/10.1098/rstb.2021.0331
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