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Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study

BACKGROUND: Sarcopenia refers to the progressive loss of skeletal muscle mass and muscle function, which seriously threatens the quality of life of the older adults. Therefore, early diagnosis is urgently needed. This study aimed to explore the changes of serum protein profiles in sarcopenia patient...

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Autores principales: Wu, Jingqiong, Cao, Longjun, Wang, Jiazhi, Wang, Yizhao, Hao, Huimin, Huang, Liping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9528053/
https://www.ncbi.nlm.nih.gov/pubmed/36192674
http://dx.doi.org/10.1186/s12891-022-05844-2
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author Wu, Jingqiong
Cao, Longjun
Wang, Jiazhi
Wang, Yizhao
Hao, Huimin
Huang, Liping
author_facet Wu, Jingqiong
Cao, Longjun
Wang, Jiazhi
Wang, Yizhao
Hao, Huimin
Huang, Liping
author_sort Wu, Jingqiong
collection PubMed
description BACKGROUND: Sarcopenia refers to the progressive loss of skeletal muscle mass and muscle function, which seriously threatens the quality of life of the older adults. Therefore, early diagnosis is urgently needed. This study aimed to explore the changes of serum protein profiles in sarcopenia patients through a cross-sectional study, and to provide the reference for clinical diagnosis. METHODS: This study was a cross-sectional study carried out in the Tianjin institute of physical education teaching experiment training center from December 2019 to December 2020. Ten older adults were recruited, including 5 sarcopenia and 5 healthy older adults. After a detailed diagnostic evaluation, blood samples were collected to prepare serum for proteomic analysis using the HPLC System Easy nLC method. The differentially expressed proteins (DEPs) were screened by the limma package of R software (version 4.1.0). RESULTS: A total of 114 DEPs were identified between the patients and healthy older adults, including 48 up-regulated proteins and 66 down-regulated proteins. The functional enrichment analysis showed that the 114 DEPs were significantly enriched in 153 GO terms, which mainly involved in low-density lipoprotein particle remodeling, and negative regulation of immune response,etc. The PPI network further suggested that the cholesteryl ester transfer protein and Apolipoprotein A2 could serve as biomarkers to facilitate diagnosis of sarcopenia. CONCLUSIONS: This study provided a serum proteomic profile of sarcopenia patients, and identified two proteins with diagnostic value, which might help to improve the diagnostic accuracy of sarcopenia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12891-022-05844-2.
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spelling pubmed-95280532022-10-04 Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study Wu, Jingqiong Cao, Longjun Wang, Jiazhi Wang, Yizhao Hao, Huimin Huang, Liping BMC Musculoskelet Disord Research BACKGROUND: Sarcopenia refers to the progressive loss of skeletal muscle mass and muscle function, which seriously threatens the quality of life of the older adults. Therefore, early diagnosis is urgently needed. This study aimed to explore the changes of serum protein profiles in sarcopenia patients through a cross-sectional study, and to provide the reference for clinical diagnosis. METHODS: This study was a cross-sectional study carried out in the Tianjin institute of physical education teaching experiment training center from December 2019 to December 2020. Ten older adults were recruited, including 5 sarcopenia and 5 healthy older adults. After a detailed diagnostic evaluation, blood samples were collected to prepare serum for proteomic analysis using the HPLC System Easy nLC method. The differentially expressed proteins (DEPs) were screened by the limma package of R software (version 4.1.0). RESULTS: A total of 114 DEPs were identified between the patients and healthy older adults, including 48 up-regulated proteins and 66 down-regulated proteins. The functional enrichment analysis showed that the 114 DEPs were significantly enriched in 153 GO terms, which mainly involved in low-density lipoprotein particle remodeling, and negative regulation of immune response,etc. The PPI network further suggested that the cholesteryl ester transfer protein and Apolipoprotein A2 could serve as biomarkers to facilitate diagnosis of sarcopenia. CONCLUSIONS: This study provided a serum proteomic profile of sarcopenia patients, and identified two proteins with diagnostic value, which might help to improve the diagnostic accuracy of sarcopenia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12891-022-05844-2. BioMed Central 2022-10-03 /pmc/articles/PMC9528053/ /pubmed/36192674 http://dx.doi.org/10.1186/s12891-022-05844-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wu, Jingqiong
Cao, Longjun
Wang, Jiazhi
Wang, Yizhao
Hao, Huimin
Huang, Liping
Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study
title Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study
title_full Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study
title_fullStr Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study
title_full_unstemmed Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study
title_short Characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study
title_sort characterization of serum protein expression profiles in the early sarcopenia older adults with low grip strength: a cross-sectional study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9528053/
https://www.ncbi.nlm.nih.gov/pubmed/36192674
http://dx.doi.org/10.1186/s12891-022-05844-2
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