Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses

Orthopoxvirus species were identified and differentiated by polymerase chain reaction amplification of genome DNA using a single primer-pair based on sequences coding for the major protein component of the cowpox virus acidophilic-type inclusion body (ATI). DNA available for 6 of 8 Old World (cowpox...

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Detalles Bibliográficos
Autores principales: Meyer, Hermann, Ropp, Susan L., Esposito, Joseph J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1997
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9528891/
https://www.ncbi.nlm.nih.gov/pubmed/9079767
http://dx.doi.org/10.1016/S0166-0934(96)02155-6
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author Meyer, Hermann
Ropp, Susan L.
Esposito, Joseph J.
author_facet Meyer, Hermann
Ropp, Susan L.
Esposito, Joseph J.
author_sort Meyer, Hermann
collection PubMed
description Orthopoxvirus species were identified and differentiated by polymerase chain reaction amplification of genome DNA using a single primer-pair based on sequences coding for the major protein component of the cowpox virus acidophilic-type inclusion body (ATI). DNA available for 6 of 8 Old World (cowpox, variola, monkeypox, camelpox, ectromelia and vaccinia viruses) and 3 New World (skunkpox, volepox, and raccoonpox) resulted in amplicons that ranged in size from 510 to 1673 base pairs depending on the species, except for raccoonpox virus DNA which did not amplify. XbaI digest gel electrophoresis profiles of the amplicons improved resolution of the differences.
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spelling pubmed-95288912022-10-07 Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses Meyer, Hermann Ropp, Susan L. Esposito, Joseph J. J Virol Methods Short Communication Orthopoxvirus species were identified and differentiated by polymerase chain reaction amplification of genome DNA using a single primer-pair based on sequences coding for the major protein component of the cowpox virus acidophilic-type inclusion body (ATI). DNA available for 6 of 8 Old World (cowpox, variola, monkeypox, camelpox, ectromelia and vaccinia viruses) and 3 New World (skunkpox, volepox, and raccoonpox) resulted in amplicons that ranged in size from 510 to 1673 base pairs depending on the species, except for raccoonpox virus DNA which did not amplify. XbaI digest gel electrophoresis profiles of the amplicons improved resolution of the differences. Published by Elsevier B.V. 1997-03 1998-01-05 /pmc/articles/PMC9528891/ /pubmed/9079767 http://dx.doi.org/10.1016/S0166-0934(96)02155-6 Text en Copyright © 1997 Published by Elsevier B.V. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.
spellingShingle Short Communication
Meyer, Hermann
Ropp, Susan L.
Esposito, Joseph J.
Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
title Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
title_full Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
title_fullStr Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
title_full_unstemmed Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
title_short Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
title_sort gene for a-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9528891/
https://www.ncbi.nlm.nih.gov/pubmed/9079767
http://dx.doi.org/10.1016/S0166-0934(96)02155-6
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AT espositojosephj geneforatypeinclusionbodyproteinisusefulforapolymerasechainreactionassaytodifferentiateorthopoxviruses

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