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Virtual 2D map of cyanobacterial proteomes

Cyanobacteria are prokaryotic Gram-negative organisms prevalent in nearly all habitats. A detailed proteomics study of Cyanobacteria has not been conducted despite extensive study of their genome sequences. Therefore, we conducted a proteome-wide analysis of the Cyanobacteria proteome and found Calo...

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Autores principales: Mohanta, Tapan Kumar, Mohanta, Yugal Kishore, Avula, Satya Kumar, Nongbet, Amilia, Al-Harrasi, Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529120/
https://www.ncbi.nlm.nih.gov/pubmed/36190972
http://dx.doi.org/10.1371/journal.pone.0275148
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author Mohanta, Tapan Kumar
Mohanta, Yugal Kishore
Avula, Satya Kumar
Nongbet, Amilia
Al-Harrasi, Ahmed
author_facet Mohanta, Tapan Kumar
Mohanta, Yugal Kishore
Avula, Satya Kumar
Nongbet, Amilia
Al-Harrasi, Ahmed
author_sort Mohanta, Tapan Kumar
collection PubMed
description Cyanobacteria are prokaryotic Gram-negative organisms prevalent in nearly all habitats. A detailed proteomics study of Cyanobacteria has not been conducted despite extensive study of their genome sequences. Therefore, we conducted a proteome-wide analysis of the Cyanobacteria proteome and found Calothrix desertica as the largest (680331.825 kDa) and Candidatus synechococcus spongiarum as the smallest (42726.77 kDa) proteome of the cyanobacterial kingdom. A Cyanobacterial proteome encodes 312.018 amino acids per protein, with a molecular weight of 182173.1324 kDa per proteome. The isoelectric point (pI) of the Cyanobacterial proteome ranges from 2.13 to 13.32. It was found that the Cyanobacterial proteome encodes a greater number of acidic-pI proteins, and their average pI is 6.437. The proteins with higher pI are likely to contain repetitive amino acids. A virtual 2D map of Cyanobacterial proteome showed a bimodal distribution of molecular weight and pI. Several proteins within the Cyanobacterial proteome were found to encode Selenocysteine (Sec) amino acid, while Pyrrolysine amino acids were not detected. The study can enable us to generate a high-resolution cell map to monitor proteomic dynamics. Through this computational analysis, we can gain a better understanding of the bias in codon usage by analyzing the amino acid composition of the Cyanobacterial proteome.
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spelling pubmed-95291202022-10-04 Virtual 2D map of cyanobacterial proteomes Mohanta, Tapan Kumar Mohanta, Yugal Kishore Avula, Satya Kumar Nongbet, Amilia Al-Harrasi, Ahmed PLoS One Research Article Cyanobacteria are prokaryotic Gram-negative organisms prevalent in nearly all habitats. A detailed proteomics study of Cyanobacteria has not been conducted despite extensive study of their genome sequences. Therefore, we conducted a proteome-wide analysis of the Cyanobacteria proteome and found Calothrix desertica as the largest (680331.825 kDa) and Candidatus synechococcus spongiarum as the smallest (42726.77 kDa) proteome of the cyanobacterial kingdom. A Cyanobacterial proteome encodes 312.018 amino acids per protein, with a molecular weight of 182173.1324 kDa per proteome. The isoelectric point (pI) of the Cyanobacterial proteome ranges from 2.13 to 13.32. It was found that the Cyanobacterial proteome encodes a greater number of acidic-pI proteins, and their average pI is 6.437. The proteins with higher pI are likely to contain repetitive amino acids. A virtual 2D map of Cyanobacterial proteome showed a bimodal distribution of molecular weight and pI. Several proteins within the Cyanobacterial proteome were found to encode Selenocysteine (Sec) amino acid, while Pyrrolysine amino acids were not detected. The study can enable us to generate a high-resolution cell map to monitor proteomic dynamics. Through this computational analysis, we can gain a better understanding of the bias in codon usage by analyzing the amino acid composition of the Cyanobacterial proteome. Public Library of Science 2022-10-03 /pmc/articles/PMC9529120/ /pubmed/36190972 http://dx.doi.org/10.1371/journal.pone.0275148 Text en © 2022 Mohanta et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mohanta, Tapan Kumar
Mohanta, Yugal Kishore
Avula, Satya Kumar
Nongbet, Amilia
Al-Harrasi, Ahmed
Virtual 2D map of cyanobacterial proteomes
title Virtual 2D map of cyanobacterial proteomes
title_full Virtual 2D map of cyanobacterial proteomes
title_fullStr Virtual 2D map of cyanobacterial proteomes
title_full_unstemmed Virtual 2D map of cyanobacterial proteomes
title_short Virtual 2D map of cyanobacterial proteomes
title_sort virtual 2d map of cyanobacterial proteomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529120/
https://www.ncbi.nlm.nih.gov/pubmed/36190972
http://dx.doi.org/10.1371/journal.pone.0275148
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