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Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement
BACKGROUND: Angiostrongylus cantonensis is the etiological agent of neuroangiostrongyliasis in humans, which is developed in gastropods and vertebrate species, mainly rodents. Human transmission occurs through consumption of molluscs and paratenic hosts infected with L3, and the migration of larvae...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Instituto Oswaldo Cruz, Ministério da Saúde
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529171/ https://www.ncbi.nlm.nih.gov/pubmed/36197401 http://dx.doi.org/10.1590/0074-02760220086 |
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author | de Melo, Leyva Cecília Vieira de Souza, Felipe Corrêa Rezende Baccin, Amanda de Oliveira da Mota, Dan Jessé Gonçalves Pereira-Chioccola, Vera Lucia Pinto, Pedro Luiz Silva |
author_facet | de Melo, Leyva Cecília Vieira de Souza, Felipe Corrêa Rezende Baccin, Amanda de Oliveira da Mota, Dan Jessé Gonçalves Pereira-Chioccola, Vera Lucia Pinto, Pedro Luiz Silva |
author_sort | de Melo, Leyva Cecília Vieira |
collection | PubMed |
description | BACKGROUND: Angiostrongylus cantonensis is the etiological agent of neuroangiostrongyliasis in humans, which is developed in gastropods and vertebrate species, mainly rodents. Human transmission occurs through consumption of molluscs and paratenic hosts infected with L3, and the migration of larvae to the central nervous system causes eosinophilic meningitis. Laboratory diagnosis is based on molecular and immunological tests, using young or adult females as a source of antigens. However, these tests give positive results only after several weeks of symptoms onset and also cross-reactions with others parasite infections may occur. OBJECTIVES: The purpose of this work was to study different antigenic preparations of distinct evolutionary phases of A. cantonensis, in order to improve serological techniques for disease immunodiagnosis. METHODS: For this purpose, antigenic fractions of different evolutionary forms were evaluated by Dot-enzyme-linked immunosorbent assay (Dot-ELISA) and Western blot using serum samples. FINDINGS: All analysed fractions showed reactivity with serum samples from patients with neuroangiostrongyliasis, especially female membrane alkaline (FAM) and female soluble alkaline (FAS) fractions together with female soluble saline (FSS), improving the technique specificity. MAIN CONCLUSIONS: The results point to the possibility of use of raw female antigens in association with alkaline membrane antigens extracted from adult worms to aid in diagnosis and helps initiate neuroangiostrongyliasis surveillance and control actions. |
format | Online Article Text |
id | pubmed-9529171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-95291712022-10-17 Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement de Melo, Leyva Cecília Vieira de Souza, Felipe Corrêa Rezende Baccin, Amanda de Oliveira da Mota, Dan Jessé Gonçalves Pereira-Chioccola, Vera Lucia Pinto, Pedro Luiz Silva Mem Inst Oswaldo Cruz Research Article BACKGROUND: Angiostrongylus cantonensis is the etiological agent of neuroangiostrongyliasis in humans, which is developed in gastropods and vertebrate species, mainly rodents. Human transmission occurs through consumption of molluscs and paratenic hosts infected with L3, and the migration of larvae to the central nervous system causes eosinophilic meningitis. Laboratory diagnosis is based on molecular and immunological tests, using young or adult females as a source of antigens. However, these tests give positive results only after several weeks of symptoms onset and also cross-reactions with others parasite infections may occur. OBJECTIVES: The purpose of this work was to study different antigenic preparations of distinct evolutionary phases of A. cantonensis, in order to improve serological techniques for disease immunodiagnosis. METHODS: For this purpose, antigenic fractions of different evolutionary forms were evaluated by Dot-enzyme-linked immunosorbent assay (Dot-ELISA) and Western blot using serum samples. FINDINGS: All analysed fractions showed reactivity with serum samples from patients with neuroangiostrongyliasis, especially female membrane alkaline (FAM) and female soluble alkaline (FAS) fractions together with female soluble saline (FSS), improving the technique specificity. MAIN CONCLUSIONS: The results point to the possibility of use of raw female antigens in association with alkaline membrane antigens extracted from adult worms to aid in diagnosis and helps initiate neuroangiostrongyliasis surveillance and control actions. Instituto Oswaldo Cruz, Ministério da Saúde 2022-10-03 /pmc/articles/PMC9529171/ /pubmed/36197401 http://dx.doi.org/10.1590/0074-02760220086 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Research Article de Melo, Leyva Cecília Vieira de Souza, Felipe Corrêa Rezende Baccin, Amanda de Oliveira da Mota, Dan Jessé Gonçalves Pereira-Chioccola, Vera Lucia Pinto, Pedro Luiz Silva Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement |
title | Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement |
title_full | Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement |
title_fullStr | Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement |
title_full_unstemmed | Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement |
title_short | Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement |
title_sort | immunoanalysis of different antigenic preparations of angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529171/ https://www.ncbi.nlm.nih.gov/pubmed/36197401 http://dx.doi.org/10.1590/0074-02760220086 |
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