Cargando…

Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity

ABSTRACT: Surfactants are used to control microbial biofilms in industrial and medical settings. Their known toxicity on aquatic biota, and their longevity in the environment, has encouraged research on biodegradable alternatives such as rhamnolipids. While previous research has investigated the eff...

Descripción completa

Detalles Bibliográficos
Autores principales: Gill, Stephanie P., Hunter, William R., Coulson, Laura E., Banat, Ibrahim M., Schelker, Jakob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529700/
https://www.ncbi.nlm.nih.gov/pubmed/36121483
http://dx.doi.org/10.1007/s00253-022-12179-4
_version_ 1784801556187578368
author Gill, Stephanie P.
Hunter, William R.
Coulson, Laura E.
Banat, Ibrahim M.
Schelker, Jakob
author_facet Gill, Stephanie P.
Hunter, William R.
Coulson, Laura E.
Banat, Ibrahim M.
Schelker, Jakob
author_sort Gill, Stephanie P.
collection PubMed
description ABSTRACT: Surfactants are used to control microbial biofilms in industrial and medical settings. Their known toxicity on aquatic biota, and their longevity in the environment, has encouraged research on biodegradable alternatives such as rhamnolipids. While previous research has investigated the effects of biological surfactants on single species biofilms, there remains a lack of information regarding the effects of synthetic and biological surfactants in freshwater ecosystems. We conducted a mesocosm experiment to test how the surfactant sodium dodecyl sulfate (SDS) and the biological surfactant rhamnolipid altered community composition and metabolic activity of freshwater biofilms. Biofilms were cultured in the flumes using lake water from Lake Lunz in Austria, under high (300 ppm) and low (150 ppm) concentrations of either surfactant over a four-week period. Our results show that both surfactants significantly affected microbial diversity. Up to 36% of microbial operational taxonomic units were lost after surfactant exposure. Rhamnolipid exposure also increased the production of the extracellular enzymes, leucine aminopeptidase, and glucosidase, while SDS exposure reduced leucine aminopeptidase and glucosidase. This study demonstrates that exposure of freshwater biofilms to chemical and biological surfactants caused a reduction of microbial diversity and changes in biofilm metabolism, exemplified by shifts in extracellular enzyme activities. KEY POINTS: • Microbial biofilm diversity decreased significantly after surfactant exposure. • Exposure to either surfactant altered extracellular enzyme activity. • Overall metabolic activity was not altered, suggesting functional redundancy.
format Online
Article
Text
id pubmed-9529700
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-95297002022-10-05 Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity Gill, Stephanie P. Hunter, William R. Coulson, Laura E. Banat, Ibrahim M. Schelker, Jakob Appl Microbiol Biotechnol Environmental Biotechnology ABSTRACT: Surfactants are used to control microbial biofilms in industrial and medical settings. Their known toxicity on aquatic biota, and their longevity in the environment, has encouraged research on biodegradable alternatives such as rhamnolipids. While previous research has investigated the effects of biological surfactants on single species biofilms, there remains a lack of information regarding the effects of synthetic and biological surfactants in freshwater ecosystems. We conducted a mesocosm experiment to test how the surfactant sodium dodecyl sulfate (SDS) and the biological surfactant rhamnolipid altered community composition and metabolic activity of freshwater biofilms. Biofilms were cultured in the flumes using lake water from Lake Lunz in Austria, under high (300 ppm) and low (150 ppm) concentrations of either surfactant over a four-week period. Our results show that both surfactants significantly affected microbial diversity. Up to 36% of microbial operational taxonomic units were lost after surfactant exposure. Rhamnolipid exposure also increased the production of the extracellular enzymes, leucine aminopeptidase, and glucosidase, while SDS exposure reduced leucine aminopeptidase and glucosidase. This study demonstrates that exposure of freshwater biofilms to chemical and biological surfactants caused a reduction of microbial diversity and changes in biofilm metabolism, exemplified by shifts in extracellular enzyme activities. KEY POINTS: • Microbial biofilm diversity decreased significantly after surfactant exposure. • Exposure to either surfactant altered extracellular enzyme activity. • Overall metabolic activity was not altered, suggesting functional redundancy. Springer Berlin Heidelberg 2022-09-19 2022 /pmc/articles/PMC9529700/ /pubmed/36121483 http://dx.doi.org/10.1007/s00253-022-12179-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Environmental Biotechnology
Gill, Stephanie P.
Hunter, William R.
Coulson, Laura E.
Banat, Ibrahim M.
Schelker, Jakob
Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity
title Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity
title_full Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity
title_fullStr Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity
title_full_unstemmed Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity
title_short Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity
title_sort synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity
topic Environmental Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529700/
https://www.ncbi.nlm.nih.gov/pubmed/36121483
http://dx.doi.org/10.1007/s00253-022-12179-4
work_keys_str_mv AT gillstephaniep syntheticandbiologicalsurfactanteffectsonfreshwaterbiofilmcommunitycompositionandmetabolicactivity
AT hunterwilliamr syntheticandbiologicalsurfactanteffectsonfreshwaterbiofilmcommunitycompositionandmetabolicactivity
AT coulsonlaurae syntheticandbiologicalsurfactanteffectsonfreshwaterbiofilmcommunitycompositionandmetabolicactivity
AT banatibrahimm syntheticandbiologicalsurfactanteffectsonfreshwaterbiofilmcommunitycompositionandmetabolicactivity
AT schelkerjakob syntheticandbiologicalsurfactanteffectsonfreshwaterbiofilmcommunitycompositionandmetabolicactivity