Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression

Osseous metastases of pulmonary carcinoma and the detailed mechanisms remain unclear, and the effects of exosomes (Exos) originated from pulmonary adenocarcinoma cells in this process have received a lot of attentions. Our study revealed that the Exos secreted from A549 cells (A549-Exos) enhanced os...

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Autores principales: Zhang, Chengcheng, Qin, Jingru, Yang, Lu, Zhu, Zhiyao, Yang, Jia, Su, Wan, Deng, Haibin, Wang, Zhongqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530222/
https://www.ncbi.nlm.nih.gov/pubmed/36192384
http://dx.doi.org/10.1038/s41420-022-01194-z
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author Zhang, Chengcheng
Qin, Jingru
Yang, Lu
Zhu, Zhiyao
Yang, Jia
Su, Wan
Deng, Haibin
Wang, Zhongqi
author_facet Zhang, Chengcheng
Qin, Jingru
Yang, Lu
Zhu, Zhiyao
Yang, Jia
Su, Wan
Deng, Haibin
Wang, Zhongqi
author_sort Zhang, Chengcheng
collection PubMed
description Osseous metastases of pulmonary carcinoma and the detailed mechanisms remain unclear, and the effects of exosomes (Exos) originated from pulmonary adenocarcinoma cells in this process have received a lot of attentions. Our study revealed that the Exos secreted from A549 cells (A549-Exos) enhanced osteoclastogenesis and osseous resorption in vitro. In addition, A549-Exos showed a targeted effect on bones to enhance osseous resorption in vivo. A549-exosomal miR-328 enhanced osseous resorption via downregulating neuropilin 2 (Nrp-2) expression, and A549-Exos miR-328 inhibitors suppressed osseous resorption in vivo. Therefore, A549-exosomal miR-328 enhances osteoclastogenesis via downregulating Nrp-2 expression, thus A549-Exos (miR-328 inhibitors) can be used as a potential nanodrug for treating osseous metastases.
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spelling pubmed-95302222022-10-05 Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression Zhang, Chengcheng Qin, Jingru Yang, Lu Zhu, Zhiyao Yang, Jia Su, Wan Deng, Haibin Wang, Zhongqi Cell Death Discov Article Osseous metastases of pulmonary carcinoma and the detailed mechanisms remain unclear, and the effects of exosomes (Exos) originated from pulmonary adenocarcinoma cells in this process have received a lot of attentions. Our study revealed that the Exos secreted from A549 cells (A549-Exos) enhanced osteoclastogenesis and osseous resorption in vitro. In addition, A549-Exos showed a targeted effect on bones to enhance osseous resorption in vivo. A549-exosomal miR-328 enhanced osseous resorption via downregulating neuropilin 2 (Nrp-2) expression, and A549-Exos miR-328 inhibitors suppressed osseous resorption in vivo. Therefore, A549-exosomal miR-328 enhances osteoclastogenesis via downregulating Nrp-2 expression, thus A549-Exos (miR-328 inhibitors) can be used as a potential nanodrug for treating osseous metastases. Nature Publishing Group UK 2022-10-03 /pmc/articles/PMC9530222/ /pubmed/36192384 http://dx.doi.org/10.1038/s41420-022-01194-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhang, Chengcheng
Qin, Jingru
Yang, Lu
Zhu, Zhiyao
Yang, Jia
Su, Wan
Deng, Haibin
Wang, Zhongqi
Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression
title Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression
title_full Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression
title_fullStr Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression
title_full_unstemmed Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression
title_short Exosomal miR-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating Nrp-2 expression
title_sort exosomal mir-328 originated from pulmonary adenocarcinoma cells enhances osteoclastogenesis via downregulating nrp-2 expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530222/
https://www.ncbi.nlm.nih.gov/pubmed/36192384
http://dx.doi.org/10.1038/s41420-022-01194-z
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