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Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice
In rice, low-temperature germinability (LTG) is essential for stable stand establishment using the direct seeding method in temperate and high-altitude areas. Previously, we reported that the quantitative trait locus qLTG1 is associated with LTG. qLTG1 is also associated with tolerance to several ab...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530605/ https://www.ncbi.nlm.nih.gov/pubmed/36204076 http://dx.doi.org/10.3389/fpls.2022.985559 |
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author | Kim, Sun Ha Shim, Kyu-Chan Lee, Hyun-Sook Jeon, Yun-A Adeva, Cheryl Luong, Ngoc Ha Ahn, Sang-Nag |
author_facet | Kim, Sun Ha Shim, Kyu-Chan Lee, Hyun-Sook Jeon, Yun-A Adeva, Cheryl Luong, Ngoc Ha Ahn, Sang-Nag |
author_sort | Kim, Sun Ha |
collection | PubMed |
description | In rice, low-temperature germinability (LTG) is essential for stable stand establishment using the direct seeding method in temperate and high-altitude areas. Previously, we reported that the quantitative trait locus qLTG1 is associated with LTG. qLTG1 is also associated with tolerance to several abiotic stresses, such as salt and osmotic conditions. In this study, map-based cloning and sequence analysis indicated that qLTG1 is allelic to DWARF2 (OsD2), which encodes cytochrome P450 D2 (LOC_Os01g10040) involved in brassinosteroid (BR) biosynthesis. Sequence comparison of the two parental lines, Hwaseong and Oryza rufipogon (IRGC 105491), revealed five single nucleotide polymorphisms (SNPs) in the coding region. Three of these SNPs led to missense mutations in OsD2, whereas the other two SNPs were synonymous. We evaluated two T-DNA insertion mutants, viz., overexpression (OsD2-OE) and knockdown (OsD2-KD) mutants of OsD2, with the Dongjin genetic background. OsD2-KD plants showed a decrease in LTG and grain size. In contrast, OsD2-OE plants showed an increase in grain size and LTG. We also examined the expression levels of several BR signaling and biosynthetic genes using the T-DNA insertion mutants. Gene expression analysis and BR application experiments demonstrated that BR enhanced the seed germination rate under low-temperature conditions. These results suggest that OsD2 is associated with the regulation of LTG and improving grain size. Thus, OsD2 may be a suitable target for rice breeding programs to improve rice yield and LTG. |
format | Online Article Text |
id | pubmed-9530605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95306052022-10-05 Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice Kim, Sun Ha Shim, Kyu-Chan Lee, Hyun-Sook Jeon, Yun-A Adeva, Cheryl Luong, Ngoc Ha Ahn, Sang-Nag Front Plant Sci Plant Science In rice, low-temperature germinability (LTG) is essential for stable stand establishment using the direct seeding method in temperate and high-altitude areas. Previously, we reported that the quantitative trait locus qLTG1 is associated with LTG. qLTG1 is also associated with tolerance to several abiotic stresses, such as salt and osmotic conditions. In this study, map-based cloning and sequence analysis indicated that qLTG1 is allelic to DWARF2 (OsD2), which encodes cytochrome P450 D2 (LOC_Os01g10040) involved in brassinosteroid (BR) biosynthesis. Sequence comparison of the two parental lines, Hwaseong and Oryza rufipogon (IRGC 105491), revealed five single nucleotide polymorphisms (SNPs) in the coding region. Three of these SNPs led to missense mutations in OsD2, whereas the other two SNPs were synonymous. We evaluated two T-DNA insertion mutants, viz., overexpression (OsD2-OE) and knockdown (OsD2-KD) mutants of OsD2, with the Dongjin genetic background. OsD2-KD plants showed a decrease in LTG and grain size. In contrast, OsD2-OE plants showed an increase in grain size and LTG. We also examined the expression levels of several BR signaling and biosynthetic genes using the T-DNA insertion mutants. Gene expression analysis and BR application experiments demonstrated that BR enhanced the seed germination rate under low-temperature conditions. These results suggest that OsD2 is associated with the regulation of LTG and improving grain size. Thus, OsD2 may be a suitable target for rice breeding programs to improve rice yield and LTG. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9530605/ /pubmed/36204076 http://dx.doi.org/10.3389/fpls.2022.985559 Text en Copyright © 2022 Kim, Shim, Lee, Jeon, Adeva, Luong and Ahn. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Kim, Sun Ha Shim, Kyu-Chan Lee, Hyun-Sook Jeon, Yun-A Adeva, Cheryl Luong, Ngoc Ha Ahn, Sang-Nag Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice |
title | Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice |
title_full | Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice |
title_fullStr | Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice |
title_full_unstemmed | Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice |
title_short | Brassinosteroid biosynthesis gene OsD2 is associated with low-temperature germinability in rice |
title_sort | brassinosteroid biosynthesis gene osd2 is associated with low-temperature germinability in rice |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530605/ https://www.ncbi.nlm.nih.gov/pubmed/36204076 http://dx.doi.org/10.3389/fpls.2022.985559 |
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