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Circulating maternal chimeric cells have an impact on the outcome of biliary atresia
INTRODUCTION: We aimed to quantify the DNA of maternal chimeric (MC) cells in the peripheral blood of the BA patients and investigated the impact on the outcome. METHODS: Patients with progressive jaundice because of no bile flow, which necessitated liver transplantation, or who showed inadequate bi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530628/ https://www.ncbi.nlm.nih.gov/pubmed/36204668 http://dx.doi.org/10.3389/fped.2022.1007927 |
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author | Masuya, Ryuta Muraji, Toshihiro Kanaan, Sami B. Harumatsu, Toshio Muto, Mitsuru Toma, Miki Yanai, Toshihiro Stevens, Anne M. Nelson, J. Lee Nakame, Kazuhiko Nanashima, Atsushi Ieiri, Satoshi |
author_facet | Masuya, Ryuta Muraji, Toshihiro Kanaan, Sami B. Harumatsu, Toshio Muto, Mitsuru Toma, Miki Yanai, Toshihiro Stevens, Anne M. Nelson, J. Lee Nakame, Kazuhiko Nanashima, Atsushi Ieiri, Satoshi |
author_sort | Masuya, Ryuta |
collection | PubMed |
description | INTRODUCTION: We aimed to quantify the DNA of maternal chimeric (MC) cells in the peripheral blood of the BA patients and investigated the impact on the outcome. METHODS: Patients with progressive jaundice because of no bile flow, which necessitated liver transplantation, or who showed inadequate bile flow with or without episodes of cholangitis and progressive hepatic fibrosis and portal hypertension were classified into the poor group. Those with adequate bile flow with completely normal liver function tests beyond 2 years were classified into the good group. The qPCR were separately carried out in buffy coat samples and plasma samples, targeting the non-inherited maternal HLA alleles in the DNA samples. RESULTS: MC-DNA was present in the buffy coat (10–328 gEq per 10(6) host cells) in seven patients. There was no MC-DNA in the remaining five patients. MC-DNA (214–15,331 gEq per 10(6) host cells) was observed in the plasma of five patients. The quantity of MC-DNA in the buffy coat showed a significant difference between the two prognostic groups (p = 0.018), whereas there was no significant difference in the quantity of MC-DNA in plasma (p = 0.205). MC-DNA in the buffy coat was significantly associated with the outcome (p = 0.028), whereas MC-DNA in the plasma did not influence the outcome (p = 0.56). CONCLUSIONS: Poor outcomes in BA were correlated with circulating maternal chimeric lymphocytes. |
format | Online Article Text |
id | pubmed-9530628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95306282022-10-05 Circulating maternal chimeric cells have an impact on the outcome of biliary atresia Masuya, Ryuta Muraji, Toshihiro Kanaan, Sami B. Harumatsu, Toshio Muto, Mitsuru Toma, Miki Yanai, Toshihiro Stevens, Anne M. Nelson, J. Lee Nakame, Kazuhiko Nanashima, Atsushi Ieiri, Satoshi Front Pediatr Pediatrics INTRODUCTION: We aimed to quantify the DNA of maternal chimeric (MC) cells in the peripheral blood of the BA patients and investigated the impact on the outcome. METHODS: Patients with progressive jaundice because of no bile flow, which necessitated liver transplantation, or who showed inadequate bile flow with or without episodes of cholangitis and progressive hepatic fibrosis and portal hypertension were classified into the poor group. Those with adequate bile flow with completely normal liver function tests beyond 2 years were classified into the good group. The qPCR were separately carried out in buffy coat samples and plasma samples, targeting the non-inherited maternal HLA alleles in the DNA samples. RESULTS: MC-DNA was present in the buffy coat (10–328 gEq per 10(6) host cells) in seven patients. There was no MC-DNA in the remaining five patients. MC-DNA (214–15,331 gEq per 10(6) host cells) was observed in the plasma of five patients. The quantity of MC-DNA in the buffy coat showed a significant difference between the two prognostic groups (p = 0.018), whereas there was no significant difference in the quantity of MC-DNA in plasma (p = 0.205). MC-DNA in the buffy coat was significantly associated with the outcome (p = 0.028), whereas MC-DNA in the plasma did not influence the outcome (p = 0.56). CONCLUSIONS: Poor outcomes in BA were correlated with circulating maternal chimeric lymphocytes. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9530628/ /pubmed/36204668 http://dx.doi.org/10.3389/fped.2022.1007927 Text en Copyright © 2022 Masuya, Muraji, Kanaan, Harumatsu, Muto, Toma, Yanai, Stevens, Nelson, Nakame, Nanashima and Ieiri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pediatrics Masuya, Ryuta Muraji, Toshihiro Kanaan, Sami B. Harumatsu, Toshio Muto, Mitsuru Toma, Miki Yanai, Toshihiro Stevens, Anne M. Nelson, J. Lee Nakame, Kazuhiko Nanashima, Atsushi Ieiri, Satoshi Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
title | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
title_full | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
title_fullStr | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
title_full_unstemmed | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
title_short | Circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
title_sort | circulating maternal chimeric cells have an impact on the outcome of biliary atresia |
topic | Pediatrics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530628/ https://www.ncbi.nlm.nih.gov/pubmed/36204668 http://dx.doi.org/10.3389/fped.2022.1007927 |
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