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Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites
BACKGROUND: Bovine metabolism undergoes significant changes during subclinical mastitis, but the relevant molecular mechanisms have not been elucidated. In this study we investigated the changes in milk microbiota and metabolites after intramammary infusion of matrine-chitosan hydrogels (MCHs) in co...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530655/ https://www.ncbi.nlm.nih.gov/pubmed/36204605 http://dx.doi.org/10.3389/fmicb.2022.950231 |
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author | Zhang, Hua Wang, Ziyue Yao, Hua Jiang, Linshu Tong, Jinjin |
author_facet | Zhang, Hua Wang, Ziyue Yao, Hua Jiang, Linshu Tong, Jinjin |
author_sort | Zhang, Hua |
collection | PubMed |
description | BACKGROUND: Bovine metabolism undergoes significant changes during subclinical mastitis, but the relevant molecular mechanisms have not been elucidated. In this study we investigated the changes in milk microbiota and metabolites after intramammary infusion of matrine-chitosan hydrogels (MCHs) in cows with subclinical mastitis. METHODS: Infusions were continued for 7 days, and milk samples were collected on days 1 and 7 for microbiome analysis by 16S rRNA gene sequencing and metabolite profiling by liquid chromatography-mass spectrometry. RESULTS: MCHs significantly decreased the somatic cell count on day 7 compared to day 1, and the Simpson index indicated that microbial diversity was significantly lower on day 7. The relative abundance of Aerococcus, Corynebacterium_1, Staphylococcus and Firmicutes was significantly decreased on day 7, while Proteobacteria increased. In the milk samples, we identified 74 differentially expressed metabolites. The MCHs infusion group had the most significantly upregulated metabolites including sphingolipids, glycerophospholipids, flavonoids and fatty acyls. The mammary gland metabolic pathways identified after MCHs treatment were consistent with the known antimicrobial and anti-inflammatory properties of matrine that are associated with glycerophospholipid metabolism and the sphingolipid metabolic signaling pathways. CONCLUSION: These insights into the immunoregulatory mechanisms and the corresponding biological responses to matrine demonstrate its potential activity in mitigating the harmful effects of bovine mastitis. |
format | Online Article Text |
id | pubmed-9530655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95306552022-10-05 Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites Zhang, Hua Wang, Ziyue Yao, Hua Jiang, Linshu Tong, Jinjin Front Microbiol Microbiology BACKGROUND: Bovine metabolism undergoes significant changes during subclinical mastitis, but the relevant molecular mechanisms have not been elucidated. In this study we investigated the changes in milk microbiota and metabolites after intramammary infusion of matrine-chitosan hydrogels (MCHs) in cows with subclinical mastitis. METHODS: Infusions were continued for 7 days, and milk samples were collected on days 1 and 7 for microbiome analysis by 16S rRNA gene sequencing and metabolite profiling by liquid chromatography-mass spectrometry. RESULTS: MCHs significantly decreased the somatic cell count on day 7 compared to day 1, and the Simpson index indicated that microbial diversity was significantly lower on day 7. The relative abundance of Aerococcus, Corynebacterium_1, Staphylococcus and Firmicutes was significantly decreased on day 7, while Proteobacteria increased. In the milk samples, we identified 74 differentially expressed metabolites. The MCHs infusion group had the most significantly upregulated metabolites including sphingolipids, glycerophospholipids, flavonoids and fatty acyls. The mammary gland metabolic pathways identified after MCHs treatment were consistent with the known antimicrobial and anti-inflammatory properties of matrine that are associated with glycerophospholipid metabolism and the sphingolipid metabolic signaling pathways. CONCLUSION: These insights into the immunoregulatory mechanisms and the corresponding biological responses to matrine demonstrate its potential activity in mitigating the harmful effects of bovine mastitis. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9530655/ /pubmed/36204605 http://dx.doi.org/10.3389/fmicb.2022.950231 Text en Copyright © 2022 Zhang, Wang, Yao, Jiang and Tong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Hua Wang, Ziyue Yao, Hua Jiang, Linshu Tong, Jinjin Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites |
title | Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites |
title_full | Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites |
title_fullStr | Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites |
title_full_unstemmed | Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites |
title_short | Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites |
title_sort | intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis —effects on milk microbiome and metabolites |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530655/ https://www.ncbi.nlm.nih.gov/pubmed/36204605 http://dx.doi.org/10.3389/fmicb.2022.950231 |
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