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Protocol for fast scRNA-seq raw data processing using scKB and non-arbitrary quality control with COPILOT

We describe a protocol to perform fast and non-arbitrary quality control of single-cell RNA sequencing (scRNA-seq) raw data using scKB and COPILOT. scKB is a wrapper script of kallisto and bustools for accelerated alignment and transcript count matrix generation, which runs significantly faster than...

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Detalles Bibliográficos
Autores principales:	Hsu, Che-Wei, Shahan, Rachel, Nolan, Trevor M., Benfey, Philip N., Ohler, Uwe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530667/ https://www.ncbi.nlm.nih.gov/pubmed/36181683 http://dx.doi.org/10.1016/j.xpro.2022.101729

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