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Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes

We investigated the possible associations between leukocyte telomere length, therapy outcomes, and clinicopathological features in patients with colorectal cancer. Additionally, telomerase reverse transcriptase (TERT) expression was evaluated. Telomere length was measured using singleplex qPCR in 47...

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Autores principales: Tomasova, Kristyna, Kroupa, Michal, Zinkova, Alzbeta, Korabecna, Marie, Vymetalkova, Veronika, Skrobanek, Pavel, Sojka, Ladislav, Levy, Miroslav, Hemminki, Kari, Liska, Vaclav, Hosek, Petr, Kumar, Rajiv, Vodickova, Ludmila, Vodicka, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530927/
https://www.ncbi.nlm.nih.gov/pubmed/36203452
http://dx.doi.org/10.3389/fonc.2022.962929
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author Tomasova, Kristyna
Kroupa, Michal
Zinkova, Alzbeta
Korabecna, Marie
Vymetalkova, Veronika
Skrobanek, Pavel
Sojka, Ladislav
Levy, Miroslav
Hemminki, Kari
Liska, Vaclav
Hosek, Petr
Kumar, Rajiv
Vodickova, Ludmila
Vodicka, Pavel
author_facet Tomasova, Kristyna
Kroupa, Michal
Zinkova, Alzbeta
Korabecna, Marie
Vymetalkova, Veronika
Skrobanek, Pavel
Sojka, Ladislav
Levy, Miroslav
Hemminki, Kari
Liska, Vaclav
Hosek, Petr
Kumar, Rajiv
Vodickova, Ludmila
Vodicka, Pavel
author_sort Tomasova, Kristyna
collection PubMed
description We investigated the possible associations between leukocyte telomere length, therapy outcomes, and clinicopathological features in patients with colorectal cancer. Additionally, telomerase reverse transcriptase (TERT) expression was evaluated. Telomere length was measured using singleplex qPCR in 478 consecutive leukocyte DNA samples from 198 patients. Blood was drawn at diagnosis prior to any therapy and then at 6-month intervals for 18 months. Following diagnosis, the telomeres gradually shortened during the course of the treatment regardless of the patient’s age. The most pronounced decrease was observed 12 months after the diagnosis (p < 0.0001). Based on tumor localization, the decrease in telomere length one year after the diagnosis followed different trajectories (p = 0.03). In patients treated with adjuvant therapy, telomere length correlated with the time elapsed after completion of therapy (p = 0.03). TERT expression did not correlate with the telomere length; however, it was higher in women than men (1.35-fold, 95% CI 1.11–1.65, p = 0.003) and in smokers than non-smokers (1.27-fold, 95% CI 1.01–1.61, p = 0.04). Leukocyte telomere length declines naturally during aging, but the accelerated shortening observed in our patients was age-independent. Telomere length manifestly reflected chemotherapy impact and could be linked to therapy toxicity.
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spelling pubmed-95309272022-10-05 Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes Tomasova, Kristyna Kroupa, Michal Zinkova, Alzbeta Korabecna, Marie Vymetalkova, Veronika Skrobanek, Pavel Sojka, Ladislav Levy, Miroslav Hemminki, Kari Liska, Vaclav Hosek, Petr Kumar, Rajiv Vodickova, Ludmila Vodicka, Pavel Front Oncol Oncology We investigated the possible associations between leukocyte telomere length, therapy outcomes, and clinicopathological features in patients with colorectal cancer. Additionally, telomerase reverse transcriptase (TERT) expression was evaluated. Telomere length was measured using singleplex qPCR in 478 consecutive leukocyte DNA samples from 198 patients. Blood was drawn at diagnosis prior to any therapy and then at 6-month intervals for 18 months. Following diagnosis, the telomeres gradually shortened during the course of the treatment regardless of the patient’s age. The most pronounced decrease was observed 12 months after the diagnosis (p < 0.0001). Based on tumor localization, the decrease in telomere length one year after the diagnosis followed different trajectories (p = 0.03). In patients treated with adjuvant therapy, telomere length correlated with the time elapsed after completion of therapy (p = 0.03). TERT expression did not correlate with the telomere length; however, it was higher in women than men (1.35-fold, 95% CI 1.11–1.65, p = 0.003) and in smokers than non-smokers (1.27-fold, 95% CI 1.01–1.61, p = 0.04). Leukocyte telomere length declines naturally during aging, but the accelerated shortening observed in our patients was age-independent. Telomere length manifestly reflected chemotherapy impact and could be linked to therapy toxicity. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9530927/ /pubmed/36203452 http://dx.doi.org/10.3389/fonc.2022.962929 Text en Copyright © 2022 Tomasova, Kroupa, Zinkova, Korabecna, Vymetalkova, Skrobanek, Sojka, Levy, Hemminki, Liska, Hosek, Kumar, Vodickova and Vodicka https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Tomasova, Kristyna
Kroupa, Michal
Zinkova, Alzbeta
Korabecna, Marie
Vymetalkova, Veronika
Skrobanek, Pavel
Sojka, Ladislav
Levy, Miroslav
Hemminki, Kari
Liska, Vaclav
Hosek, Petr
Kumar, Rajiv
Vodickova, Ludmila
Vodicka, Pavel
Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes
title Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes
title_full Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes
title_fullStr Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes
title_full_unstemmed Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes
title_short Monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes
title_sort monitoring of telomere dynamics in peripheral blood leukocytes in relation to colorectal cancer patients’ outcomes
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530927/
https://www.ncbi.nlm.nih.gov/pubmed/36203452
http://dx.doi.org/10.3389/fonc.2022.962929
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