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Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection
People Living with HIV (PLHIV) are at an increased risk of pneumococcal pneumonia than HIV-uninfected adults, but the reasons for this are still not well understood. We investigated whether alveolar macrophages (AM) mediated control of pneumococcal infection is impaired in PLHIV compared to HIV-unin...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531125/ https://www.ncbi.nlm.nih.gov/pubmed/36203580 http://dx.doi.org/10.3389/fimmu.2022.992659 |
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author | Nyazika, Tinashe K. Sibale, Lusako Phiri, Joseph De Ste Croix, Megan Jasiunaite, Zydrune Mkandawire, Christopher Malamba, Rose Kankwatira, Anstead Manduwa, Miriam Ferreira, Daniela M. Nyirenda, Tonney S. Oggioni, Marco R. Mwandumba, Henry C. Jambo, Kondwani C. |
author_facet | Nyazika, Tinashe K. Sibale, Lusako Phiri, Joseph De Ste Croix, Megan Jasiunaite, Zydrune Mkandawire, Christopher Malamba, Rose Kankwatira, Anstead Manduwa, Miriam Ferreira, Daniela M. Nyirenda, Tonney S. Oggioni, Marco R. Mwandumba, Henry C. Jambo, Kondwani C. |
author_sort | Nyazika, Tinashe K. |
collection | PubMed |
description | People Living with HIV (PLHIV) are at an increased risk of pneumococcal pneumonia than HIV-uninfected adults, but the reasons for this are still not well understood. We investigated whether alveolar macrophages (AM) mediated control of pneumococcal infection is impaired in PLHIV compared to HIV-uninfected adults. We assessed anti-bactericidal activity against Streptococcus pneumoniae of primary human AM obtained from PLHIV and HIV-uninfected adults. We found that pneumococcus survived intracellularly in AMs at least 24 hours post ex vivo infection, and this was more frequent in PLHIV than HIV-uninfected adults. Corroborating these findings, in vivo evidence showed that PLHIV had a higher propensity for harboring S. pneumoniae within their AMs than HIV-uninfected adults. Moreover, bacterial intracellular survival in AMs was associated with extracellular propagation of pneumococcal infection. Our data suggest that failure of AMs to eliminate S. pneumoniae intracellularly could contribute to the increased risk of pneumococcal pneumonia in PLHIV. |
format | Online Article Text |
id | pubmed-9531125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95311252022-10-05 Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection Nyazika, Tinashe K. Sibale, Lusako Phiri, Joseph De Ste Croix, Megan Jasiunaite, Zydrune Mkandawire, Christopher Malamba, Rose Kankwatira, Anstead Manduwa, Miriam Ferreira, Daniela M. Nyirenda, Tonney S. Oggioni, Marco R. Mwandumba, Henry C. Jambo, Kondwani C. Front Immunol Immunology People Living with HIV (PLHIV) are at an increased risk of pneumococcal pneumonia than HIV-uninfected adults, but the reasons for this are still not well understood. We investigated whether alveolar macrophages (AM) mediated control of pneumococcal infection is impaired in PLHIV compared to HIV-uninfected adults. We assessed anti-bactericidal activity against Streptococcus pneumoniae of primary human AM obtained from PLHIV and HIV-uninfected adults. We found that pneumococcus survived intracellularly in AMs at least 24 hours post ex vivo infection, and this was more frequent in PLHIV than HIV-uninfected adults. Corroborating these findings, in vivo evidence showed that PLHIV had a higher propensity for harboring S. pneumoniae within their AMs than HIV-uninfected adults. Moreover, bacterial intracellular survival in AMs was associated with extracellular propagation of pneumococcal infection. Our data suggest that failure of AMs to eliminate S. pneumoniae intracellularly could contribute to the increased risk of pneumococcal pneumonia in PLHIV. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9531125/ /pubmed/36203580 http://dx.doi.org/10.3389/fimmu.2022.992659 Text en Copyright © 2022 Nyazika, Sibale, Phiri, De Ste Croix, Jasiunaite, Mkandawire, Malamba, Kankwatira, Manduwa, Ferreira, Nyirenda, Oggioni, Mwandumba and Jambo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Nyazika, Tinashe K. Sibale, Lusako Phiri, Joseph De Ste Croix, Megan Jasiunaite, Zydrune Mkandawire, Christopher Malamba, Rose Kankwatira, Anstead Manduwa, Miriam Ferreira, Daniela M. Nyirenda, Tonney S. Oggioni, Marco R. Mwandumba, Henry C. Jambo, Kondwani C. Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection |
title | Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection |
title_full | Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection |
title_fullStr | Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection |
title_full_unstemmed | Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection |
title_short | Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection |
title_sort | intracellular survival of streptococcus pneumoniae in human alveolar macrophages is augmented with hiv infection |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531125/ https://www.ncbi.nlm.nih.gov/pubmed/36203580 http://dx.doi.org/10.3389/fimmu.2022.992659 |
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