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Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile

Ex vivo phenotyping of P. falciparum erythrocyte invasion diversity is important in the identification and down selection of potential malaria vaccine targets. However, due to the lack of appropriate laboratory facilities in remote areas of endemic countries, direct processing of P. falciparum clini...

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Autores principales: Thiam, Laty G., Ansah, Felix, Niang, Makhtar, Awandare, Gordon A., Aniweh, Yaw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531135/
https://www.ncbi.nlm.nih.gov/pubmed/36204649
http://dx.doi.org/10.3389/fcimb.2022.997418
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author Thiam, Laty G.
Ansah, Felix
Niang, Makhtar
Awandare, Gordon A.
Aniweh, Yaw
author_facet Thiam, Laty G.
Ansah, Felix
Niang, Makhtar
Awandare, Gordon A.
Aniweh, Yaw
author_sort Thiam, Laty G.
collection PubMed
description Ex vivo phenotyping of P. falciparum erythrocyte invasion diversity is important in the identification and down selection of potential malaria vaccine targets. However, due to the lack of appropriate laboratory facilities in remote areas of endemic countries, direct processing of P. falciparum clinical isolates is usually not feasible. Here, we investigated the combined effect of short-term cryopreservation and thawing processes on the ex vivo invasion phenotypes of P. falciparum isolates. Ex-vivo or in vitro invasion phenotyping assays were performed with P. falciparum clinical isolates prior to or following culture adaptation, respectively. All isolates were genotyped at Day 0 for parasite clonality. Subsequently, isolates that were successfully culture-adapted were genotyped again at Days 7, 15, 21, and 28-post adaptation. Invasion phenotyping assays were performed in isogenic isolates revived at different time points (3, 6, and 12 months) post-cryopreservation and the resulting data were compared to that from ex-vivo invasion data of matched isogenic parental isolates. We also show that short-term culture adaptation selects for parasite clonality and could be a driving force for variation in invasion phenotypes as compared to ex vivo data where almost all parasite clones of a given isolate are present. Interestingly, our data show little variation in the parasites’ invasion phenotype following short-term cryopreservation. Altogether, our data suggest that short-term cryopreservation of uncultured P. falciparum clinical isolates is a reliable mechanism for storing parasites for future use.
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spelling pubmed-95311352022-10-05 Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile Thiam, Laty G. Ansah, Felix Niang, Makhtar Awandare, Gordon A. Aniweh, Yaw Front Cell Infect Microbiol Cellular and Infection Microbiology Ex vivo phenotyping of P. falciparum erythrocyte invasion diversity is important in the identification and down selection of potential malaria vaccine targets. However, due to the lack of appropriate laboratory facilities in remote areas of endemic countries, direct processing of P. falciparum clinical isolates is usually not feasible. Here, we investigated the combined effect of short-term cryopreservation and thawing processes on the ex vivo invasion phenotypes of P. falciparum isolates. Ex-vivo or in vitro invasion phenotyping assays were performed with P. falciparum clinical isolates prior to or following culture adaptation, respectively. All isolates were genotyped at Day 0 for parasite clonality. Subsequently, isolates that were successfully culture-adapted were genotyped again at Days 7, 15, 21, and 28-post adaptation. Invasion phenotyping assays were performed in isogenic isolates revived at different time points (3, 6, and 12 months) post-cryopreservation and the resulting data were compared to that from ex-vivo invasion data of matched isogenic parental isolates. We also show that short-term culture adaptation selects for parasite clonality and could be a driving force for variation in invasion phenotypes as compared to ex vivo data where almost all parasite clones of a given isolate are present. Interestingly, our data show little variation in the parasites’ invasion phenotype following short-term cryopreservation. Altogether, our data suggest that short-term cryopreservation of uncultured P. falciparum clinical isolates is a reliable mechanism for storing parasites for future use. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9531135/ /pubmed/36204649 http://dx.doi.org/10.3389/fcimb.2022.997418 Text en Copyright © 2022 Thiam, Ansah, Niang, Awandare and Aniweh https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Thiam, Laty G.
Ansah, Felix
Niang, Makhtar
Awandare, Gordon A.
Aniweh, Yaw
Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile
title Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile
title_full Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile
title_fullStr Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile
title_full_unstemmed Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile
title_short Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile
title_sort short-term cryopreservation and thawing have minimal effects on plasmodium falciparum ex vivo invasion profile
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531135/
https://www.ncbi.nlm.nih.gov/pubmed/36204649
http://dx.doi.org/10.3389/fcimb.2022.997418
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