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Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis

The oocyte is a unique cell, from which develops a complex organism comprising of germ layers, tissues and organs. In some vertebrate species it is known that the asymmetrical localization of biomolecules within the oocyte is what drives the spatial differentiation of the daughter cells required for...

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Autores principales: Iegorova, Viktoriia, Naraine, Ravindra, Psenicka, Martin, Zelazowska, Monika, Sindelka, Radek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531136/
https://www.ncbi.nlm.nih.gov/pubmed/36204678
http://dx.doi.org/10.3389/fcell.2022.982732
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author Iegorova, Viktoriia
Naraine, Ravindra
Psenicka, Martin
Zelazowska, Monika
Sindelka, Radek
author_facet Iegorova, Viktoriia
Naraine, Ravindra
Psenicka, Martin
Zelazowska, Monika
Sindelka, Radek
author_sort Iegorova, Viktoriia
collection PubMed
description The oocyte is a unique cell, from which develops a complex organism comprising of germ layers, tissues and organs. In some vertebrate species it is known that the asymmetrical localization of biomolecules within the oocyte is what drives the spatial differentiation of the daughter cells required for embryogenesis. This asymmetry is first established to produce an animal-vegetal (A-V) axis which reflects the future specification of the ectoderm, mesoderm, and endoderm layers. Several pathways for localization of vegetal maternal transcripts have already been described using a few animal models. However, there is limited information about transcripts that are localized to the animal pole, even though there is accumulating evidence indicating its active establishment. Here, we performed comparative TOMO-Seq analysis on two holoblastic cleavage models: Xenopus laevis and Acipenser ruthenus oocytes during oogenesis. We found that there were many transcripts that have a temporal preference for the establishment of localization. In both models, we observed vegetal transcript gradients that were established during either the early or late oogenesis stages and transcripts that started their localization during the early stages but became more pronounced during the later stages. We found that some animal gradients were already established during the early stages, however the majority were formed during the later stages of oogenesis. Some of these temporally localized transcripts were conserved between the models, while others were species specific. Additionally, temporal de novo transcription and also degradation of transcripts within the oocyte were observed, pointing to an active remodeling of the maternal RNA pool.
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spelling pubmed-95311362022-10-05 Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis Iegorova, Viktoriia Naraine, Ravindra Psenicka, Martin Zelazowska, Monika Sindelka, Radek Front Cell Dev Biol Cell and Developmental Biology The oocyte is a unique cell, from which develops a complex organism comprising of germ layers, tissues and organs. In some vertebrate species it is known that the asymmetrical localization of biomolecules within the oocyte is what drives the spatial differentiation of the daughter cells required for embryogenesis. This asymmetry is first established to produce an animal-vegetal (A-V) axis which reflects the future specification of the ectoderm, mesoderm, and endoderm layers. Several pathways for localization of vegetal maternal transcripts have already been described using a few animal models. However, there is limited information about transcripts that are localized to the animal pole, even though there is accumulating evidence indicating its active establishment. Here, we performed comparative TOMO-Seq analysis on two holoblastic cleavage models: Xenopus laevis and Acipenser ruthenus oocytes during oogenesis. We found that there were many transcripts that have a temporal preference for the establishment of localization. In both models, we observed vegetal transcript gradients that were established during either the early or late oogenesis stages and transcripts that started their localization during the early stages but became more pronounced during the later stages. We found that some animal gradients were already established during the early stages, however the majority were formed during the later stages of oogenesis. Some of these temporally localized transcripts were conserved between the models, while others were species specific. Additionally, temporal de novo transcription and also degradation of transcripts within the oocyte were observed, pointing to an active remodeling of the maternal RNA pool. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9531136/ /pubmed/36204678 http://dx.doi.org/10.3389/fcell.2022.982732 Text en Copyright © 2022 Iegorova, Naraine, Psenicka, Zelazowska and Sindelka. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Iegorova, Viktoriia
Naraine, Ravindra
Psenicka, Martin
Zelazowska, Monika
Sindelka, Radek
Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
title Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
title_full Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
title_fullStr Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
title_full_unstemmed Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
title_short Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
title_sort comparison of rna localization during oogenesis within acipenser ruthenus and xenopus laevis
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531136/
https://www.ncbi.nlm.nih.gov/pubmed/36204678
http://dx.doi.org/10.3389/fcell.2022.982732
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