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Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection
N(6) -Methyladenosine (m(6)A), the most abundant mammalian mRNA modification, has been reported to modulate various viral infections. Although it has been confirmed that RNA modifications can also modulate the replication and development of different parasites, the role of the RNA epitranscriptome i...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531237/ https://www.ncbi.nlm.nih.gov/pubmed/36203583 http://dx.doi.org/10.3389/fimmu.2022.998756 |
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author | Wang, Luoluo Wu, Jian Liu, Runzhou Chen, Wenjun Pang, Zhichang Zhou, Fan Xia, Lu Huang, Jia Pan, Tao Su, Xin-zhuan Wang, Xiaoyun |
author_facet | Wang, Luoluo Wu, Jian Liu, Runzhou Chen, Wenjun Pang, Zhichang Zhou, Fan Xia, Lu Huang, Jia Pan, Tao Su, Xin-zhuan Wang, Xiaoyun |
author_sort | Wang, Luoluo |
collection | PubMed |
description | N(6) -Methyladenosine (m(6)A), the most abundant mammalian mRNA modification, has been reported to modulate various viral infections. Although it has been confirmed that RNA modifications can also modulate the replication and development of different parasites, the role of the RNA epitranscriptome in the regulation of host response post parasite infection remains to be elucidated. Here we report host spleen m(6)A epitranscriptome landscapes induced by different strains of the malaria parasite Plasmodium yoelii. We found that malaria parasite infection dramatically changes host spleen m(6)A mRNA modification and gene expression. Additionally, malaria parasite infection reprograms host immune response pathways by regulating the m(6)A modification enzymes. Collectively, our study is the first characterization of host spleen m(6)A methylome triggered by malaria parasite infections, and our data identify m(6)A modifications as significant transcriptome-wide marks during host-parasite interactions. We demonstrate that host mRNA methylation machinery can sense and respond to malaria parasite infections, and provide new insights into epitranscriptomic mechanisms underlying parasite-induced pathogenesis. |
format | Online Article Text |
id | pubmed-9531237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95312372022-10-05 Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection Wang, Luoluo Wu, Jian Liu, Runzhou Chen, Wenjun Pang, Zhichang Zhou, Fan Xia, Lu Huang, Jia Pan, Tao Su, Xin-zhuan Wang, Xiaoyun Front Immunol Immunology N(6) -Methyladenosine (m(6)A), the most abundant mammalian mRNA modification, has been reported to modulate various viral infections. Although it has been confirmed that RNA modifications can also modulate the replication and development of different parasites, the role of the RNA epitranscriptome in the regulation of host response post parasite infection remains to be elucidated. Here we report host spleen m(6)A epitranscriptome landscapes induced by different strains of the malaria parasite Plasmodium yoelii. We found that malaria parasite infection dramatically changes host spleen m(6)A mRNA modification and gene expression. Additionally, malaria parasite infection reprograms host immune response pathways by regulating the m(6)A modification enzymes. Collectively, our study is the first characterization of host spleen m(6)A methylome triggered by malaria parasite infections, and our data identify m(6)A modifications as significant transcriptome-wide marks during host-parasite interactions. We demonstrate that host mRNA methylation machinery can sense and respond to malaria parasite infections, and provide new insights into epitranscriptomic mechanisms underlying parasite-induced pathogenesis. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9531237/ /pubmed/36203583 http://dx.doi.org/10.3389/fimmu.2022.998756 Text en Copyright © 2022 Wang, Wu, Liu, Chen, Pang, Zhou, Xia, Huang, Pan, Su and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Wang, Luoluo Wu, Jian Liu, Runzhou Chen, Wenjun Pang, Zhichang Zhou, Fan Xia, Lu Huang, Jia Pan, Tao Su, Xin-zhuan Wang, Xiaoyun Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection |
title | Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection |
title_full | Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection |
title_fullStr | Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection |
title_full_unstemmed | Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection |
title_short | Epitranscriptome profiling of spleen mRNA m(6)A methylation reveals pathways of host responses to malaria parasite infection |
title_sort | epitranscriptome profiling of spleen mrna m(6)a methylation reveals pathways of host responses to malaria parasite infection |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531237/ https://www.ncbi.nlm.nih.gov/pubmed/36203583 http://dx.doi.org/10.3389/fimmu.2022.998756 |
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