Cargando…

Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens

Silver nanoparticles (AgNPs) are a powerful disinfectant, but little information is available on their potential use as a growth promoter and the safety margin of this. In this study, 480 1-day-old Cobb chicks were assigned to one control and three treated groups. The treated groups were supplemente...

Descripción completa

Detalles Bibliográficos
Autores principales: Khafaga, Asmaa F., Fouda, Moustafa M. G., Alwan, Ali B., Abdelsalam, Nader R., Taha, Ayman E., Atta, Mustafa S., Dosoky, Waleed M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531355/
https://www.ncbi.nlm.nih.gov/pubmed/36195872
http://dx.doi.org/10.1186/s12917-022-03459-2
_version_ 1784801884659253248
author Khafaga, Asmaa F.
Fouda, Moustafa M. G.
Alwan, Ali B.
Abdelsalam, Nader R.
Taha, Ayman E.
Atta, Mustafa S.
Dosoky, Waleed M.
author_facet Khafaga, Asmaa F.
Fouda, Moustafa M. G.
Alwan, Ali B.
Abdelsalam, Nader R.
Taha, Ayman E.
Atta, Mustafa S.
Dosoky, Waleed M.
author_sort Khafaga, Asmaa F.
collection PubMed
description Silver nanoparticles (AgNPs) are a powerful disinfectant, but little information is available on their potential use as a growth promoter and the safety margin of this. In this study, 480 1-day-old Cobb chicks were assigned to one control and three treated groups. The treated groups were supplemented with silver-doped silica nanoparticles (SiO(2)@AgNPs) at three dietary levels (8, 16, and 20 mg/kg diet) for 35 days. The results revealed no significant changes in the growth performance and oxidative parameters, and in most of the hematological and biochemical parameters among the control and treated groups. In contrast, dose-dependent adverse effects were exerted on the histopathological structure and immunohistochemical expression of CD45 in liver, kidneys, and lymphoid organs (spleen, bursa, and thymus). In addition, the relative weight of lymphoid organs and the serum levels of immunoglobulins M and G were significantly diminished. Moreover, the gene expression of proinflammatory cytokines (IL-β1 and TNF-α) and the ultrastructural morphology in breast muscle showed significant dose-dependent alterations. It could be concluded that the dietary supplementation of SiO(2)@AgNPs at a level of 8 mg/kg diet or more has dose-dependent proinflammatory and immunosuppressive effects on broiler chickens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03459-2.
format Online
Article
Text
id pubmed-9531355
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-95313552022-10-05 Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens Khafaga, Asmaa F. Fouda, Moustafa M. G. Alwan, Ali B. Abdelsalam, Nader R. Taha, Ayman E. Atta, Mustafa S. Dosoky, Waleed M. BMC Vet Res Research Silver nanoparticles (AgNPs) are a powerful disinfectant, but little information is available on their potential use as a growth promoter and the safety margin of this. In this study, 480 1-day-old Cobb chicks were assigned to one control and three treated groups. The treated groups were supplemented with silver-doped silica nanoparticles (SiO(2)@AgNPs) at three dietary levels (8, 16, and 20 mg/kg diet) for 35 days. The results revealed no significant changes in the growth performance and oxidative parameters, and in most of the hematological and biochemical parameters among the control and treated groups. In contrast, dose-dependent adverse effects were exerted on the histopathological structure and immunohistochemical expression of CD45 in liver, kidneys, and lymphoid organs (spleen, bursa, and thymus). In addition, the relative weight of lymphoid organs and the serum levels of immunoglobulins M and G were significantly diminished. Moreover, the gene expression of proinflammatory cytokines (IL-β1 and TNF-α) and the ultrastructural morphology in breast muscle showed significant dose-dependent alterations. It could be concluded that the dietary supplementation of SiO(2)@AgNPs at a level of 8 mg/kg diet or more has dose-dependent proinflammatory and immunosuppressive effects on broiler chickens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03459-2. BioMed Central 2022-10-04 /pmc/articles/PMC9531355/ /pubmed/36195872 http://dx.doi.org/10.1186/s12917-022-03459-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Khafaga, Asmaa F.
Fouda, Moustafa M. G.
Alwan, Ali B.
Abdelsalam, Nader R.
Taha, Ayman E.
Atta, Mustafa S.
Dosoky, Waleed M.
Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens
title Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens
title_full Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens
title_fullStr Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens
title_full_unstemmed Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens
title_short Silver-Silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens
title_sort silver-silica nanoparticles induced dose-dependent modulation of histopathological, immunohistochemical, ultrastructural, proinflammatory, and immune status of broiler chickens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531355/
https://www.ncbi.nlm.nih.gov/pubmed/36195872
http://dx.doi.org/10.1186/s12917-022-03459-2
work_keys_str_mv AT khafagaasmaaf silversilicananoparticlesinduceddosedependentmodulationofhistopathologicalimmunohistochemicalultrastructuralproinflammatoryandimmunestatusofbroilerchickens
AT foudamoustafamg silversilicananoparticlesinduceddosedependentmodulationofhistopathologicalimmunohistochemicalultrastructuralproinflammatoryandimmunestatusofbroilerchickens
AT alwanalib silversilicananoparticlesinduceddosedependentmodulationofhistopathologicalimmunohistochemicalultrastructuralproinflammatoryandimmunestatusofbroilerchickens
AT abdelsalamnaderr silversilicananoparticlesinduceddosedependentmodulationofhistopathologicalimmunohistochemicalultrastructuralproinflammatoryandimmunestatusofbroilerchickens
AT tahaaymane silversilicananoparticlesinduceddosedependentmodulationofhistopathologicalimmunohistochemicalultrastructuralproinflammatoryandimmunestatusofbroilerchickens
AT attamustafas silversilicananoparticlesinduceddosedependentmodulationofhistopathologicalimmunohistochemicalultrastructuralproinflammatoryandimmunestatusofbroilerchickens
AT dosokywaleedm silversilicananoparticlesinduceddosedependentmodulationofhistopathologicalimmunohistochemicalultrastructuralproinflammatoryandimmunestatusofbroilerchickens