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Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide

OBJECTIVE: To investigate the influences of Astragalus polysaccharides (APS) on the expressions of SR-BI(Scavenger receptor-BI) and LXR α in RAW264.7 macrophage origin foam cells. METHODS: Mouse RAW264.7 cells were induced in foam cells and identification of the foamed by oil-red O staining. The RAW...

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Detalles Bibliográficos
Autores principales: Yan, Lichen, Guo, Jing, Gao, Xiaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532143/
https://www.ncbi.nlm.nih.gov/pubmed/36247857
http://dx.doi.org/10.1155/2022/3920584
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author Yan, Lichen
Guo, Jing
Gao, Xiaohua
author_facet Yan, Lichen
Guo, Jing
Gao, Xiaohua
author_sort Yan, Lichen
collection PubMed
description OBJECTIVE: To investigate the influences of Astragalus polysaccharides (APS) on the expressions of SR-BI(Scavenger receptor-BI) and LXR α in RAW264.7 macrophage origin foam cells. METHODS: Mouse RAW264.7 cells were induced in foam cells and identification of the foamed by oil-red O staining. The RAW264.7 macrophage origin foam cells were dealt with APS with distinct contents (0, 10, 20, 50, or 100 mg/L). The mRNA and protein expressions of SR-BI and LXRα were measured by RT-PCR as well as ELISA, respectively. RESULTS: Macrophages were differentiated into foam cells 48 h after ox-LDL induction. In contrast to the control part, the mRNA and protein expressions of SR-BI and LXRα in RAW264.7 macrophage origin foam cells were up-regulated dosage-dependently after being treated with different concentrations of APS (P < 0.05). CONCLUSION: APS could promote intracellular cholesterol efflux by up-regulating the expressions of SR-BI and LXR α of RAW264.7 cells.
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spelling pubmed-95321432022-10-14 Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide Yan, Lichen Guo, Jing Gao, Xiaohua Contrast Media Mol Imaging Research Article OBJECTIVE: To investigate the influences of Astragalus polysaccharides (APS) on the expressions of SR-BI(Scavenger receptor-BI) and LXR α in RAW264.7 macrophage origin foam cells. METHODS: Mouse RAW264.7 cells were induced in foam cells and identification of the foamed by oil-red O staining. The RAW264.7 macrophage origin foam cells were dealt with APS with distinct contents (0, 10, 20, 50, or 100 mg/L). The mRNA and protein expressions of SR-BI and LXRα were measured by RT-PCR as well as ELISA, respectively. RESULTS: Macrophages were differentiated into foam cells 48 h after ox-LDL induction. In contrast to the control part, the mRNA and protein expressions of SR-BI and LXRα in RAW264.7 macrophage origin foam cells were up-regulated dosage-dependently after being treated with different concentrations of APS (P < 0.05). CONCLUSION: APS could promote intracellular cholesterol efflux by up-regulating the expressions of SR-BI and LXR α of RAW264.7 cells. Hindawi 2022-09-27 /pmc/articles/PMC9532143/ /pubmed/36247857 http://dx.doi.org/10.1155/2022/3920584 Text en Copyright © 2022 Lichen Yan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yan, Lichen
Guo, Jing
Gao, Xiaohua
Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide
title Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide
title_full Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide
title_fullStr Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide
title_full_unstemmed Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide
title_short Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide
title_sort expression of sr-bi and lxrα in macrophage origin foam cells of mouse raw264.7 up-regulated by astragalus polysaccharide
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532143/
https://www.ncbi.nlm.nih.gov/pubmed/36247857
http://dx.doi.org/10.1155/2022/3920584
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