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Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide
OBJECTIVE: To investigate the influences of Astragalus polysaccharides (APS) on the expressions of SR-BI(Scavenger receptor-BI) and LXR α in RAW264.7 macrophage origin foam cells. METHODS: Mouse RAW264.7 cells were induced in foam cells and identification of the foamed by oil-red O staining. The RAW...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532143/ https://www.ncbi.nlm.nih.gov/pubmed/36247857 http://dx.doi.org/10.1155/2022/3920584 |
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author | Yan, Lichen Guo, Jing Gao, Xiaohua |
author_facet | Yan, Lichen Guo, Jing Gao, Xiaohua |
author_sort | Yan, Lichen |
collection | PubMed |
description | OBJECTIVE: To investigate the influences of Astragalus polysaccharides (APS) on the expressions of SR-BI(Scavenger receptor-BI) and LXR α in RAW264.7 macrophage origin foam cells. METHODS: Mouse RAW264.7 cells were induced in foam cells and identification of the foamed by oil-red O staining. The RAW264.7 macrophage origin foam cells were dealt with APS with distinct contents (0, 10, 20, 50, or 100 mg/L). The mRNA and protein expressions of SR-BI and LXRα were measured by RT-PCR as well as ELISA, respectively. RESULTS: Macrophages were differentiated into foam cells 48 h after ox-LDL induction. In contrast to the control part, the mRNA and protein expressions of SR-BI and LXRα in RAW264.7 macrophage origin foam cells were up-regulated dosage-dependently after being treated with different concentrations of APS (P < 0.05). CONCLUSION: APS could promote intracellular cholesterol efflux by up-regulating the expressions of SR-BI and LXR α of RAW264.7 cells. |
format | Online Article Text |
id | pubmed-9532143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95321432022-10-14 Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide Yan, Lichen Guo, Jing Gao, Xiaohua Contrast Media Mol Imaging Research Article OBJECTIVE: To investigate the influences of Astragalus polysaccharides (APS) on the expressions of SR-BI(Scavenger receptor-BI) and LXR α in RAW264.7 macrophage origin foam cells. METHODS: Mouse RAW264.7 cells were induced in foam cells and identification of the foamed by oil-red O staining. The RAW264.7 macrophage origin foam cells were dealt with APS with distinct contents (0, 10, 20, 50, or 100 mg/L). The mRNA and protein expressions of SR-BI and LXRα were measured by RT-PCR as well as ELISA, respectively. RESULTS: Macrophages were differentiated into foam cells 48 h after ox-LDL induction. In contrast to the control part, the mRNA and protein expressions of SR-BI and LXRα in RAW264.7 macrophage origin foam cells were up-regulated dosage-dependently after being treated with different concentrations of APS (P < 0.05). CONCLUSION: APS could promote intracellular cholesterol efflux by up-regulating the expressions of SR-BI and LXR α of RAW264.7 cells. Hindawi 2022-09-27 /pmc/articles/PMC9532143/ /pubmed/36247857 http://dx.doi.org/10.1155/2022/3920584 Text en Copyright © 2022 Lichen Yan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yan, Lichen Guo, Jing Gao, Xiaohua Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide |
title | Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide |
title_full | Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide |
title_fullStr | Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide |
title_full_unstemmed | Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide |
title_short | Expression of SR-BI and LXRα in Macrophage Origin Foam Cells of Mouse RAW264.7 Up-regulated by Astragalus Polysaccharide |
title_sort | expression of sr-bi and lxrα in macrophage origin foam cells of mouse raw264.7 up-regulated by astragalus polysaccharide |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532143/ https://www.ncbi.nlm.nih.gov/pubmed/36247857 http://dx.doi.org/10.1155/2022/3920584 |
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