Cargando…
Magnetofection approach for the transformation of okra using green iron nanoparticles
Climate change, pesticide resistance, and the need for developing new plant varieties have galvanized biotechnologists to find new solutions in order to produce transgenic plants. Over the last decade scientists are working on green metallic nanoparticles to develop DNA delivery systems for plants....
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532403/ https://www.ncbi.nlm.nih.gov/pubmed/36195624 http://dx.doi.org/10.1038/s41598-022-20569-x |
_version_ | 1784802108333096960 |
---|---|
author | Farooq, Naila Ather, Laraib Shafiq, Muhammad Nawaz-ul-Rehman, Muhammad Shah Haseeb, Muhammad Anjum, Tehmina Abbas, Qamar Hussain, Mujahid Ali, Numan Asad Abbas, Syed Agha Armaghan Mushtaq, Sehrish Haider, Muhammad Saleem Sadiq, Saleha Shahid, Muhammad Adnan |
author_facet | Farooq, Naila Ather, Laraib Shafiq, Muhammad Nawaz-ul-Rehman, Muhammad Shah Haseeb, Muhammad Anjum, Tehmina Abbas, Qamar Hussain, Mujahid Ali, Numan Asad Abbas, Syed Agha Armaghan Mushtaq, Sehrish Haider, Muhammad Saleem Sadiq, Saleha Shahid, Muhammad Adnan |
author_sort | Farooq, Naila |
collection | PubMed |
description | Climate change, pesticide resistance, and the need for developing new plant varieties have galvanized biotechnologists to find new solutions in order to produce transgenic plants. Over the last decade scientists are working on green metallic nanoparticles to develop DNA delivery systems for plants. In the current study, green Iron nanoparticles were synthesized using leaf extract of Camellia sinensis (green tea) and Iron Chloride (FeCl(3)), the characterization and Confirmation was done using UV–VIS Spectroscopy, FTIR, SEM, and TEM. Using these nanoparticles, a novel method of gene transformation in okra plants was developed, with a combination of different Magnetofection factors. Maximum gene transformation efficiency was observed at the DNA to Iron-nanoparticles ratio of 1:20, by rotation of mixture (Plasmid DNA, Iron-nanoparticles, and seed embryo) at 800 rpm for 5 h. Using this approach, the transformation of the GFP (green fluorescent protein) gene was successfully carried out in Abelmoschus esculentus (Okra plant). The DNA transformation was confirmed by observing the expression of transgene GFP via Laser Scanning Confocal Microscope (LSCM) and PCR. This method is highly economical, adaptable, genotype independent, eco-friendly, and time-saving as well. We infer that this approach can be a potential solution to combat the yield and immunity challenges of plants against pathogens. |
format | Online Article Text |
id | pubmed-9532403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95324032022-10-06 Magnetofection approach for the transformation of okra using green iron nanoparticles Farooq, Naila Ather, Laraib Shafiq, Muhammad Nawaz-ul-Rehman, Muhammad Shah Haseeb, Muhammad Anjum, Tehmina Abbas, Qamar Hussain, Mujahid Ali, Numan Asad Abbas, Syed Agha Armaghan Mushtaq, Sehrish Haider, Muhammad Saleem Sadiq, Saleha Shahid, Muhammad Adnan Sci Rep Article Climate change, pesticide resistance, and the need for developing new plant varieties have galvanized biotechnologists to find new solutions in order to produce transgenic plants. Over the last decade scientists are working on green metallic nanoparticles to develop DNA delivery systems for plants. In the current study, green Iron nanoparticles were synthesized using leaf extract of Camellia sinensis (green tea) and Iron Chloride (FeCl(3)), the characterization and Confirmation was done using UV–VIS Spectroscopy, FTIR, SEM, and TEM. Using these nanoparticles, a novel method of gene transformation in okra plants was developed, with a combination of different Magnetofection factors. Maximum gene transformation efficiency was observed at the DNA to Iron-nanoparticles ratio of 1:20, by rotation of mixture (Plasmid DNA, Iron-nanoparticles, and seed embryo) at 800 rpm for 5 h. Using this approach, the transformation of the GFP (green fluorescent protein) gene was successfully carried out in Abelmoschus esculentus (Okra plant). The DNA transformation was confirmed by observing the expression of transgene GFP via Laser Scanning Confocal Microscope (LSCM) and PCR. This method is highly economical, adaptable, genotype independent, eco-friendly, and time-saving as well. We infer that this approach can be a potential solution to combat the yield and immunity challenges of plants against pathogens. Nature Publishing Group UK 2022-10-04 /pmc/articles/PMC9532403/ /pubmed/36195624 http://dx.doi.org/10.1038/s41598-022-20569-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Farooq, Naila Ather, Laraib Shafiq, Muhammad Nawaz-ul-Rehman, Muhammad Shah Haseeb, Muhammad Anjum, Tehmina Abbas, Qamar Hussain, Mujahid Ali, Numan Asad Abbas, Syed Agha Armaghan Mushtaq, Sehrish Haider, Muhammad Saleem Sadiq, Saleha Shahid, Muhammad Adnan Magnetofection approach for the transformation of okra using green iron nanoparticles |
title | Magnetofection approach for the transformation of okra using green iron nanoparticles |
title_full | Magnetofection approach for the transformation of okra using green iron nanoparticles |
title_fullStr | Magnetofection approach for the transformation of okra using green iron nanoparticles |
title_full_unstemmed | Magnetofection approach for the transformation of okra using green iron nanoparticles |
title_short | Magnetofection approach for the transformation of okra using green iron nanoparticles |
title_sort | magnetofection approach for the transformation of okra using green iron nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532403/ https://www.ncbi.nlm.nih.gov/pubmed/36195624 http://dx.doi.org/10.1038/s41598-022-20569-x |
work_keys_str_mv | AT farooqnaila magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT atherlaraib magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT shafiqmuhammad magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT nawazulrehmanmuhammadshah magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT haseebmuhammad magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT anjumtehmina magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT abbasqamar magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT hussainmujahid magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT alinuman magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT asadabbassyedaghaarmaghan magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT mushtaqsehrish magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT haidermuhammadsaleem magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT sadiqsaleha magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles AT shahidmuhammadadnan magnetofectionapproachforthetransformationofokrausinggreenironnanoparticles |