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Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder

Neurexin1 gene is essential for formulating synaptic cell adhesion to establish synapses. In a previous work, 38 SNPs in Neurexin1 recoded in mental disorder patients have been collected. Five computational prediction tools have been used to predict the effect of SNPs on protein function and stabili...

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Autores principales: Hendam, Ashraf, Al-Sadek, Ahmed Farouk, Hefny, Hesham Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532826/
https://www.ncbi.nlm.nih.gov/pubmed/36197641
http://dx.doi.org/10.1007/s12031-022-02072-0
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author Hendam, Ashraf
Al-Sadek, Ahmed Farouk
Hefny, Hesham Ahmed
author_facet Hendam, Ashraf
Al-Sadek, Ahmed Farouk
Hefny, Hesham Ahmed
author_sort Hendam, Ashraf
collection PubMed
description Neurexin1 gene is essential for formulating synaptic cell adhesion to establish synapses. In a previous work, 38 SNPs in Neurexin1 recoded in mental disorder patients have been collected. Five computational prediction tools have been used to predict the effect of SNPs on protein function and stability. Only four SNPs in Neurexin1α have deleterious prediction results from at least four tools. The current work aims to use molecular dynamic simulation (MD) to study the effects of the four mutations on Neurexin1α both on the whole protein as well as identifying affected domains by mutations. A protein model that consists of five domains out of six domains in the real protein was used; missing residues were added, and model was tested for quality. The MD experiment has last for 1.5 μs where four parameters have been used for studying the whole protein in addition to three more parameters for the domain analysis. The whole protein study has shown that two mutations E427I for Autism and R525C for non-syndromic intellectual disability (NSID) have distinctive behavior across the four used parameters. Domain study has confirmed the previous results where the five domains of R525C have acted differently from wild type (WT), while E427I has acted differently for four domains from wild type. The other two mutations D104H and G379E have three domains that only acted differently from wild type. The fourth domain of all mutations has an obvious distinctive behavior from wild type. Further study of E427I and R525C mutations can lead to better understanding of autism and NSID.
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spelling pubmed-95328262022-10-05 Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder Hendam, Ashraf Al-Sadek, Ahmed Farouk Hefny, Hesham Ahmed J Mol Neurosci Article Neurexin1 gene is essential for formulating synaptic cell adhesion to establish synapses. In a previous work, 38 SNPs in Neurexin1 recoded in mental disorder patients have been collected. Five computational prediction tools have been used to predict the effect of SNPs on protein function and stability. Only four SNPs in Neurexin1α have deleterious prediction results from at least four tools. The current work aims to use molecular dynamic simulation (MD) to study the effects of the four mutations on Neurexin1α both on the whole protein as well as identifying affected domains by mutations. A protein model that consists of five domains out of six domains in the real protein was used; missing residues were added, and model was tested for quality. The MD experiment has last for 1.5 μs where four parameters have been used for studying the whole protein in addition to three more parameters for the domain analysis. The whole protein study has shown that two mutations E427I for Autism and R525C for non-syndromic intellectual disability (NSID) have distinctive behavior across the four used parameters. Domain study has confirmed the previous results where the five domains of R525C have acted differently from wild type (WT), while E427I has acted differently for four domains from wild type. The other two mutations D104H and G379E have three domains that only acted differently from wild type. The fourth domain of all mutations has an obvious distinctive behavior from wild type. Further study of E427I and R525C mutations can lead to better understanding of autism and NSID. Springer US 2022-10-05 2022 /pmc/articles/PMC9532826/ /pubmed/36197641 http://dx.doi.org/10.1007/s12031-022-02072-0 Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Hendam, Ashraf
Al-Sadek, Ahmed Farouk
Hefny, Hesham Ahmed
Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder
title Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder
title_full Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder
title_fullStr Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder
title_full_unstemmed Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder
title_short Molecular Dynamic Simulation of Neurexin1α Mutations Associated with Mental Disorder
title_sort molecular dynamic simulation of neurexin1α mutations associated with mental disorder
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532826/
https://www.ncbi.nlm.nih.gov/pubmed/36197641
http://dx.doi.org/10.1007/s12031-022-02072-0
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