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Biohybrid elastin-like venous valve with potential for in situ tissue engineering

Chronic venous insufficiency (CVI) is a leading vascular disease whose clinical manifestations include varicose veins, edemas, venous ulcers, and venous hypertension, among others. Therapies targeting this medical issue are scarce, and so far, no single venous valve prosthesis is clinically availabl...

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Autores principales: González-Pérez, Fernando, Acosta, Sergio, Rütten, Stephan, Emonts, Caroline, Kopp, Alexander, Henke, Heinz-Werner, Bruners, Philipp, Gries, Thomas, Rodríguez-Cabello, J. Carlos, Jockenhoevel, Stefan, Fernández-Colino, Alicia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532864/
https://www.ncbi.nlm.nih.gov/pubmed/36213079
http://dx.doi.org/10.3389/fbioe.2022.988533
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author González-Pérez, Fernando
Acosta, Sergio
Rütten, Stephan
Emonts, Caroline
Kopp, Alexander
Henke, Heinz-Werner
Bruners, Philipp
Gries, Thomas
Rodríguez-Cabello, J. Carlos
Jockenhoevel, Stefan
Fernández-Colino, Alicia
author_facet González-Pérez, Fernando
Acosta, Sergio
Rütten, Stephan
Emonts, Caroline
Kopp, Alexander
Henke, Heinz-Werner
Bruners, Philipp
Gries, Thomas
Rodríguez-Cabello, J. Carlos
Jockenhoevel, Stefan
Fernández-Colino, Alicia
author_sort González-Pérez, Fernando
collection PubMed
description Chronic venous insufficiency (CVI) is a leading vascular disease whose clinical manifestations include varicose veins, edemas, venous ulcers, and venous hypertension, among others. Therapies targeting this medical issue are scarce, and so far, no single venous valve prosthesis is clinically available. Herein, we have designed a bi-leaflet transcatheter venous valve that consists of (i) elastin-like recombinamers, (ii) a textile mesh reinforcement, and (iii) a bioabsorbable magnesium stent structure. Mechanical characterization of the resulting biohybrid elastin-like venous valves (EVV) showed an anisotropic behavior equivalent to the native bovine saphenous vein valves and mechanical strength suitable for vascular implantation. The EVV also featured minimal hemolysis and platelet adhesion, besides actively supporting endothelialization in vitro, thus setting the basis for its application as an in situ tissue engineering implant. In addition, the hydrodynamic testing in a pulsatile bioreactor demonstrated excellent hemodynamic valve performance, with minimal regurgitation (<10%) and pressure drop (<5 mmHg). No stagnation points were detected and an in vitro simulated transcatheter delivery showed the ability of the venous valve to withstand the implantation procedure. These results present a promising concept of a biohybrid transcatheter venous valve as an off-the-shelf implant, with great potential to provide clinical solutions for CVI treatment.
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spelling pubmed-95328642022-10-06 Biohybrid elastin-like venous valve with potential for in situ tissue engineering González-Pérez, Fernando Acosta, Sergio Rütten, Stephan Emonts, Caroline Kopp, Alexander Henke, Heinz-Werner Bruners, Philipp Gries, Thomas Rodríguez-Cabello, J. Carlos Jockenhoevel, Stefan Fernández-Colino, Alicia Front Bioeng Biotechnol Bioengineering and Biotechnology Chronic venous insufficiency (CVI) is a leading vascular disease whose clinical manifestations include varicose veins, edemas, venous ulcers, and venous hypertension, among others. Therapies targeting this medical issue are scarce, and so far, no single venous valve prosthesis is clinically available. Herein, we have designed a bi-leaflet transcatheter venous valve that consists of (i) elastin-like recombinamers, (ii) a textile mesh reinforcement, and (iii) a bioabsorbable magnesium stent structure. Mechanical characterization of the resulting biohybrid elastin-like venous valves (EVV) showed an anisotropic behavior equivalent to the native bovine saphenous vein valves and mechanical strength suitable for vascular implantation. The EVV also featured minimal hemolysis and platelet adhesion, besides actively supporting endothelialization in vitro, thus setting the basis for its application as an in situ tissue engineering implant. In addition, the hydrodynamic testing in a pulsatile bioreactor demonstrated excellent hemodynamic valve performance, with minimal regurgitation (<10%) and pressure drop (<5 mmHg). No stagnation points were detected and an in vitro simulated transcatheter delivery showed the ability of the venous valve to withstand the implantation procedure. These results present a promising concept of a biohybrid transcatheter venous valve as an off-the-shelf implant, with great potential to provide clinical solutions for CVI treatment. Frontiers Media S.A. 2022-09-21 /pmc/articles/PMC9532864/ /pubmed/36213079 http://dx.doi.org/10.3389/fbioe.2022.988533 Text en Copyright © 2022 González-Pérez, Acosta, Rütten, Emonts, Kopp, Henke, Bruners, Gries, Rodríguez-Cabello, Jockenhoevel and Fernández-Colino. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
González-Pérez, Fernando
Acosta, Sergio
Rütten, Stephan
Emonts, Caroline
Kopp, Alexander
Henke, Heinz-Werner
Bruners, Philipp
Gries, Thomas
Rodríguez-Cabello, J. Carlos
Jockenhoevel, Stefan
Fernández-Colino, Alicia
Biohybrid elastin-like venous valve with potential for in situ tissue engineering
title Biohybrid elastin-like venous valve with potential for in situ tissue engineering
title_full Biohybrid elastin-like venous valve with potential for in situ tissue engineering
title_fullStr Biohybrid elastin-like venous valve with potential for in situ tissue engineering
title_full_unstemmed Biohybrid elastin-like venous valve with potential for in situ tissue engineering
title_short Biohybrid elastin-like venous valve with potential for in situ tissue engineering
title_sort biohybrid elastin-like venous valve with potential for in situ tissue engineering
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532864/
https://www.ncbi.nlm.nih.gov/pubmed/36213079
http://dx.doi.org/10.3389/fbioe.2022.988533
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