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Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus

Opisthopappus longilobus, which is a unique wild plant resource in China, produces leaves and flowers with distinct aromas. However, there have been relatively few molecular studies on its floral aroma, which has hindered the research on this plant species at the molecular level and the breeding of...

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Autores principales: Liu, Hua, Chai, Yuhong, Chen, Haixia, Chen, Wendan, Li, Yushu, Liu, Wenchao, Guo, Shuang, Sun, Lei, Zhou, Xiumei, Huang, Conglin, Tang, Xiaowei, Luo, Chang, Chen, Dongliang, Cheng, Xi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533026/
https://www.ncbi.nlm.nih.gov/pubmed/36212386
http://dx.doi.org/10.3389/fpls.2022.1015942
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author Liu, Hua
Chai, Yuhong
Chen, Haixia
Chen, Wendan
Li, Yushu
Liu, Wenchao
Guo, Shuang
Sun, Lei
Zhou, Xiumei
Huang, Conglin
Tang, Xiaowei
Luo, Chang
Chen, Dongliang
Cheng, Xi
author_facet Liu, Hua
Chai, Yuhong
Chen, Haixia
Chen, Wendan
Li, Yushu
Liu, Wenchao
Guo, Shuang
Sun, Lei
Zhou, Xiumei
Huang, Conglin
Tang, Xiaowei
Luo, Chang
Chen, Dongliang
Cheng, Xi
author_sort Liu, Hua
collection PubMed
description Opisthopappus longilobus, which is a unique wild plant resource in China, produces leaves and flowers with distinct aromas. However, there have been relatively few molecular studies on its floral aroma, which has hindered the research on this plant species at the molecular level and the breeding of novel varieties. In this study, transcriptome and metabolome analyses were performed using O. longilobus leaves, buds, and inflorescences at the exposure, initial opening, and blooming stages. Using high-quality reads and assembly software, a total of 45,674 unigenes were annotated according to the Nr, Swiss-Prot, KOG, and KEGG databases. Additionally, a GC-MS system and a self-built database were used to detect 1,371 metabolites in the leaves, buds, and inflorescences. Terpene metabolites were the most common compounds (308 in total). We analyzed the gene network regulating terpenoid accumulation in O. longilobus and identified 56 candidate genes related to terpenoid synthesis. The expression of OlPMK2, OlMVK1, OlTPS1, and OlTPS3 may lead to the accumulation of 11 different terpenoids specifically in the inflorescences at the exposure, initial opening, and blooming stages. The generated data may be useful for future research on O. longilobus genetic resources and the molecular mechanism regulating aroma formation in this plant species. The findings of this study may be used to accelerate the breeding of new O. longilobus varieties with enhanced aromatic traits.
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spelling pubmed-95330262022-10-06 Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus Liu, Hua Chai, Yuhong Chen, Haixia Chen, Wendan Li, Yushu Liu, Wenchao Guo, Shuang Sun, Lei Zhou, Xiumei Huang, Conglin Tang, Xiaowei Luo, Chang Chen, Dongliang Cheng, Xi Front Plant Sci Plant Science Opisthopappus longilobus, which is a unique wild plant resource in China, produces leaves and flowers with distinct aromas. However, there have been relatively few molecular studies on its floral aroma, which has hindered the research on this plant species at the molecular level and the breeding of novel varieties. In this study, transcriptome and metabolome analyses were performed using O. longilobus leaves, buds, and inflorescences at the exposure, initial opening, and blooming stages. Using high-quality reads and assembly software, a total of 45,674 unigenes were annotated according to the Nr, Swiss-Prot, KOG, and KEGG databases. Additionally, a GC-MS system and a self-built database were used to detect 1,371 metabolites in the leaves, buds, and inflorescences. Terpene metabolites were the most common compounds (308 in total). We analyzed the gene network regulating terpenoid accumulation in O. longilobus and identified 56 candidate genes related to terpenoid synthesis. The expression of OlPMK2, OlMVK1, OlTPS1, and OlTPS3 may lead to the accumulation of 11 different terpenoids specifically in the inflorescences at the exposure, initial opening, and blooming stages. The generated data may be useful for future research on O. longilobus genetic resources and the molecular mechanism regulating aroma formation in this plant species. The findings of this study may be used to accelerate the breeding of new O. longilobus varieties with enhanced aromatic traits. Frontiers Media S.A. 2022-09-21 /pmc/articles/PMC9533026/ /pubmed/36212386 http://dx.doi.org/10.3389/fpls.2022.1015942 Text en Copyright © 2022 Liu, Chai, Chen, Chen, Li, Liu, Guo, Sun, Zhou, Huang, Tang, Luo, Chen and Cheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Hua
Chai, Yuhong
Chen, Haixia
Chen, Wendan
Li, Yushu
Liu, Wenchao
Guo, Shuang
Sun, Lei
Zhou, Xiumei
Huang, Conglin
Tang, Xiaowei
Luo, Chang
Chen, Dongliang
Cheng, Xi
Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus
title Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus
title_full Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus
title_fullStr Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus
title_full_unstemmed Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus
title_short Analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of Opisthopappus longilobus
title_sort analysis of terpenoids and their gene regulatory networks on the basis of the transcriptome and metabolome of opisthopappus longilobus
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533026/
https://www.ncbi.nlm.nih.gov/pubmed/36212386
http://dx.doi.org/10.3389/fpls.2022.1015942
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