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Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. To...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533096/ https://www.ncbi.nlm.nih.gov/pubmed/36213411 http://dx.doi.org/10.3389/fvets.2022.993442 |
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author | Machado, Isadora F. Magalhães, Edison S. Poeta Silva, Ana Paula S. Moraes, Daniel C. A. Cezar, Guilherme Mil-Homens, Mafalda P. Osemeke, Onyekachukwu H. Paiva, Rodrigo Moura, Cesar A. A. Gauger, Phillip Trevisan, Giovani Silva, Gustavo S. Linhares, Daniel C. L. |
author_facet | Machado, Isadora F. Magalhães, Edison S. Poeta Silva, Ana Paula S. Moraes, Daniel C. A. Cezar, Guilherme Mil-Homens, Mafalda P. Osemeke, Onyekachukwu H. Paiva, Rodrigo Moura, Cesar A. A. Gauger, Phillip Trevisan, Giovani Silva, Gustavo S. Linhares, Daniel C. L. |
author_sort | Machado, Isadora F. |
collection | PubMed |
description | The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. Tongue tips and serum samples were collected from three PRRSV-positive breeding herd farms (farms A, B, and C) of three different age groups: newborns (<24 h), processing (2 to 7 days of age), and weaning (18 to 22 days of age). Additionally, processing fluids and family oral fluids were collected from 2–7 days of age and weaning age, respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all age groups (100 and 95%, respectively). In addition, PRRSV RNA was detected in pooled serum samples (42 and 27%), processing fluids (100 and 50%), and family oral fluids (11 and 22%). Interestingly, the average Ct value from tongue tips was numerically lower than the average Ct value from serum samples in the newborn age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected in tongue tips when pooled serum samples from the same age group tested PRRSV RNA-negative. Taken together, these results demonstrate the potential value of tongue tips for PRRSV monitoring and surveillance. |
format | Online Article Text |
id | pubmed-9533096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95330962022-10-06 Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals Machado, Isadora F. Magalhães, Edison S. Poeta Silva, Ana Paula S. Moraes, Daniel C. A. Cezar, Guilherme Mil-Homens, Mafalda P. Osemeke, Onyekachukwu H. Paiva, Rodrigo Moura, Cesar A. A. Gauger, Phillip Trevisan, Giovani Silva, Gustavo S. Linhares, Daniel C. L. Front Vet Sci Veterinary Science The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. Tongue tips and serum samples were collected from three PRRSV-positive breeding herd farms (farms A, B, and C) of three different age groups: newborns (<24 h), processing (2 to 7 days of age), and weaning (18 to 22 days of age). Additionally, processing fluids and family oral fluids were collected from 2–7 days of age and weaning age, respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all age groups (100 and 95%, respectively). In addition, PRRSV RNA was detected in pooled serum samples (42 and 27%), processing fluids (100 and 50%), and family oral fluids (11 and 22%). Interestingly, the average Ct value from tongue tips was numerically lower than the average Ct value from serum samples in the newborn age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected in tongue tips when pooled serum samples from the same age group tested PRRSV RNA-negative. Taken together, these results demonstrate the potential value of tongue tips for PRRSV monitoring and surveillance. Frontiers Media S.A. 2022-09-21 /pmc/articles/PMC9533096/ /pubmed/36213411 http://dx.doi.org/10.3389/fvets.2022.993442 Text en Copyright © 2022 Machado, Magalhães, Poeta Silva, Moraes, Cezar, Mil-Homens, Osemeke, Paiva, Moura, Gauger, Trevisan, Silva and Linhares. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Machado, Isadora F. Magalhães, Edison S. Poeta Silva, Ana Paula S. Moraes, Daniel C. A. Cezar, Guilherme Mil-Homens, Mafalda P. Osemeke, Onyekachukwu H. Paiva, Rodrigo Moura, Cesar A. A. Gauger, Phillip Trevisan, Giovani Silva, Gustavo S. Linhares, Daniel C. L. Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals |
title | Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals |
title_full | Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals |
title_fullStr | Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals |
title_full_unstemmed | Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals |
title_short | Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals |
title_sort | porcine reproductive and respiratory syndrome virus rna detection in tongue tips from dead animals |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533096/ https://www.ncbi.nlm.nih.gov/pubmed/36213411 http://dx.doi.org/10.3389/fvets.2022.993442 |
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