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Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals

The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. To...

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Autores principales: Machado, Isadora F., Magalhães, Edison S., Poeta Silva, Ana Paula S., Moraes, Daniel C. A., Cezar, Guilherme, Mil-Homens, Mafalda P., Osemeke, Onyekachukwu H., Paiva, Rodrigo, Moura, Cesar A. A., Gauger, Phillip, Trevisan, Giovani, Silva, Gustavo S., Linhares, Daniel C. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533096/
https://www.ncbi.nlm.nih.gov/pubmed/36213411
http://dx.doi.org/10.3389/fvets.2022.993442
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author Machado, Isadora F.
Magalhães, Edison S.
Poeta Silva, Ana Paula S.
Moraes, Daniel C. A.
Cezar, Guilherme
Mil-Homens, Mafalda P.
Osemeke, Onyekachukwu H.
Paiva, Rodrigo
Moura, Cesar A. A.
Gauger, Phillip
Trevisan, Giovani
Silva, Gustavo S.
Linhares, Daniel C. L.
author_facet Machado, Isadora F.
Magalhães, Edison S.
Poeta Silva, Ana Paula S.
Moraes, Daniel C. A.
Cezar, Guilherme
Mil-Homens, Mafalda P.
Osemeke, Onyekachukwu H.
Paiva, Rodrigo
Moura, Cesar A. A.
Gauger, Phillip
Trevisan, Giovani
Silva, Gustavo S.
Linhares, Daniel C. L.
author_sort Machado, Isadora F.
collection PubMed
description The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. Tongue tips and serum samples were collected from three PRRSV-positive breeding herd farms (farms A, B, and C) of three different age groups: newborns (<24 h), processing (2 to 7 days of age), and weaning (18 to 22 days of age). Additionally, processing fluids and family oral fluids were collected from 2–7 days of age and weaning age, respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all age groups (100 and 95%, respectively). In addition, PRRSV RNA was detected in pooled serum samples (42 and 27%), processing fluids (100 and 50%), and family oral fluids (11 and 22%). Interestingly, the average Ct value from tongue tips was numerically lower than the average Ct value from serum samples in the newborn age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected in tongue tips when pooled serum samples from the same age group tested PRRSV RNA-negative. Taken together, these results demonstrate the potential value of tongue tips for PRRSV monitoring and surveillance.
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spelling pubmed-95330962022-10-06 Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals Machado, Isadora F. Magalhães, Edison S. Poeta Silva, Ana Paula S. Moraes, Daniel C. A. Cezar, Guilherme Mil-Homens, Mafalda P. Osemeke, Onyekachukwu H. Paiva, Rodrigo Moura, Cesar A. A. Gauger, Phillip Trevisan, Giovani Silva, Gustavo S. Linhares, Daniel C. L. Front Vet Sci Veterinary Science The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. Tongue tips and serum samples were collected from three PRRSV-positive breeding herd farms (farms A, B, and C) of three different age groups: newborns (<24 h), processing (2 to 7 days of age), and weaning (18 to 22 days of age). Additionally, processing fluids and family oral fluids were collected from 2–7 days of age and weaning age, respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all age groups (100 and 95%, respectively). In addition, PRRSV RNA was detected in pooled serum samples (42 and 27%), processing fluids (100 and 50%), and family oral fluids (11 and 22%). Interestingly, the average Ct value from tongue tips was numerically lower than the average Ct value from serum samples in the newborn age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected in tongue tips when pooled serum samples from the same age group tested PRRSV RNA-negative. Taken together, these results demonstrate the potential value of tongue tips for PRRSV monitoring and surveillance. Frontiers Media S.A. 2022-09-21 /pmc/articles/PMC9533096/ /pubmed/36213411 http://dx.doi.org/10.3389/fvets.2022.993442 Text en Copyright © 2022 Machado, Magalhães, Poeta Silva, Moraes, Cezar, Mil-Homens, Osemeke, Paiva, Moura, Gauger, Trevisan, Silva and Linhares. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Machado, Isadora F.
Magalhães, Edison S.
Poeta Silva, Ana Paula S.
Moraes, Daniel C. A.
Cezar, Guilherme
Mil-Homens, Mafalda P.
Osemeke, Onyekachukwu H.
Paiva, Rodrigo
Moura, Cesar A. A.
Gauger, Phillip
Trevisan, Giovani
Silva, Gustavo S.
Linhares, Daniel C. L.
Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
title Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
title_full Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
title_fullStr Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
title_full_unstemmed Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
title_short Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals
title_sort porcine reproductive and respiratory syndrome virus rna detection in tongue tips from dead animals
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533096/
https://www.ncbi.nlm.nih.gov/pubmed/36213411
http://dx.doi.org/10.3389/fvets.2022.993442
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