Cargando…

mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study

BACKGROUND: A comparative analysis of protein expression of gingival crevicular fluid (GCF) obtained from healthy individuals and individuals with periodontal diseases would help to identify proteins involved in periodontal disease progression. Among the identified proteins, Moesin which is a diseas...

Descripción completa

Detalles Bibliográficos
Autores principales: Cecil, Anju, Sambashivaiah, Savita, Bilichodmath, Shivaprasad, John, Rubin S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533393/
https://www.ncbi.nlm.nih.gov/pubmed/36213856
http://dx.doi.org/10.4103/ccd.ccd_6_21
_version_ 1784802335152668672
author Cecil, Anju
Sambashivaiah, Savita
Bilichodmath, Shivaprasad
John, Rubin S.
author_facet Cecil, Anju
Sambashivaiah, Savita
Bilichodmath, Shivaprasad
John, Rubin S.
author_sort Cecil, Anju
collection PubMed
description BACKGROUND: A comparative analysis of protein expression of gingival crevicular fluid (GCF) obtained from healthy individuals and individuals with periodontal diseases would help to identify proteins involved in periodontal disease progression. Among the identified proteins, Moesin which is a disease-associated protein belongs to the ezrin-radixin-moesin protein family and was proved to play an important role in the recognition of oral bacteria contributing to the consequent development of inflammatory immune responses involved in periodontal disease development. AIM: The aim of the study is to quantify and compare mRNA expression levels of ezrin in GCF and whole blood of gingivitis and chronic periodontitis patients. MATERIALS AND METHODS: A total of 60 patients were selected for the study and were divided into three groups as follows: Group 1 (20 participants with healthy gingiva), Group 2 (20 participants with gingivitis), and Group 3 (20 participants with chronic periodontitis). Clinical parameters such as gingival index, periodontal index, probing pocket depth, and clinical attachment level were assessed. GCF and blood samples were taken from these patients and assessed for the mRNA expression of ezrin using real-time polymerase chain reaction. RESULTS: The expression and mean relative quantification of mRNA expression of ezrin in GCF and blood were higher for periodontitis (18.32 ± 8.398, 19.34 ± 9.487) when compared to that of gingivitis (5.34 ± 3.609, 5.48 ± 4.428) and healthy individuals (2.33 ± 0.643, 3.47 ± 1.923) and they positively correlated with the clinical parameters. CONCLUSION: The increased expression of ezrin can be considered as a good indicator to assess the inflammatory activity in periodontitis and gingivitis.
format Online
Article
Text
id pubmed-9533393
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-95333932022-10-06 mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study Cecil, Anju Sambashivaiah, Savita Bilichodmath, Shivaprasad John, Rubin S. Contemp Clin Dent Original Article BACKGROUND: A comparative analysis of protein expression of gingival crevicular fluid (GCF) obtained from healthy individuals and individuals with periodontal diseases would help to identify proteins involved in periodontal disease progression. Among the identified proteins, Moesin which is a disease-associated protein belongs to the ezrin-radixin-moesin protein family and was proved to play an important role in the recognition of oral bacteria contributing to the consequent development of inflammatory immune responses involved in periodontal disease development. AIM: The aim of the study is to quantify and compare mRNA expression levels of ezrin in GCF and whole blood of gingivitis and chronic periodontitis patients. MATERIALS AND METHODS: A total of 60 patients were selected for the study and were divided into three groups as follows: Group 1 (20 participants with healthy gingiva), Group 2 (20 participants with gingivitis), and Group 3 (20 participants with chronic periodontitis). Clinical parameters such as gingival index, periodontal index, probing pocket depth, and clinical attachment level were assessed. GCF and blood samples were taken from these patients and assessed for the mRNA expression of ezrin using real-time polymerase chain reaction. RESULTS: The expression and mean relative quantification of mRNA expression of ezrin in GCF and blood were higher for periodontitis (18.32 ± 8.398, 19.34 ± 9.487) when compared to that of gingivitis (5.34 ± 3.609, 5.48 ± 4.428) and healthy individuals (2.33 ± 0.643, 3.47 ± 1.923) and they positively correlated with the clinical parameters. CONCLUSION: The increased expression of ezrin can be considered as a good indicator to assess the inflammatory activity in periodontitis and gingivitis. Medknow Publications & Media Pvt Ltd 2022 2022-09-24 /pmc/articles/PMC9533393/ /pubmed/36213856 http://dx.doi.org/10.4103/ccd.ccd_6_21 Text en Copyright: © 2022 Contemporary Clinical Dentistry https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Cecil, Anju
Sambashivaiah, Savita
Bilichodmath, Shivaprasad
John, Rubin S.
mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study
title mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study
title_full mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study
title_fullStr mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study
title_full_unstemmed mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study
title_short mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study
title_sort mrna expression of ezrin in gingival crevicular fluid and whole blood of gingivitis and chronic periodontitis patients – a polymerase chain reaction study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533393/
https://www.ncbi.nlm.nih.gov/pubmed/36213856
http://dx.doi.org/10.4103/ccd.ccd_6_21
work_keys_str_mv AT cecilanju mrnaexpressionofezriningingivalcrevicularfluidandwholebloodofgingivitisandchronicperiodontitispatientsapolymerasechainreactionstudy
AT sambashivaiahsavita mrnaexpressionofezriningingivalcrevicularfluidandwholebloodofgingivitisandchronicperiodontitispatientsapolymerasechainreactionstudy
AT bilichodmathshivaprasad mrnaexpressionofezriningingivalcrevicularfluidandwholebloodofgingivitisandchronicperiodontitispatientsapolymerasechainreactionstudy
AT johnrubins mrnaexpressionofezriningingivalcrevicularfluidandwholebloodofgingivitisandchronicperiodontitispatientsapolymerasechainreactionstudy