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mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study
BACKGROUND: A comparative analysis of protein expression of gingival crevicular fluid (GCF) obtained from healthy individuals and individuals with periodontal diseases would help to identify proteins involved in periodontal disease progression. Among the identified proteins, Moesin which is a diseas...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533393/ https://www.ncbi.nlm.nih.gov/pubmed/36213856 http://dx.doi.org/10.4103/ccd.ccd_6_21 |
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author | Cecil, Anju Sambashivaiah, Savita Bilichodmath, Shivaprasad John, Rubin S. |
author_facet | Cecil, Anju Sambashivaiah, Savita Bilichodmath, Shivaprasad John, Rubin S. |
author_sort | Cecil, Anju |
collection | PubMed |
description | BACKGROUND: A comparative analysis of protein expression of gingival crevicular fluid (GCF) obtained from healthy individuals and individuals with periodontal diseases would help to identify proteins involved in periodontal disease progression. Among the identified proteins, Moesin which is a disease-associated protein belongs to the ezrin-radixin-moesin protein family and was proved to play an important role in the recognition of oral bacteria contributing to the consequent development of inflammatory immune responses involved in periodontal disease development. AIM: The aim of the study is to quantify and compare mRNA expression levels of ezrin in GCF and whole blood of gingivitis and chronic periodontitis patients. MATERIALS AND METHODS: A total of 60 patients were selected for the study and were divided into three groups as follows: Group 1 (20 participants with healthy gingiva), Group 2 (20 participants with gingivitis), and Group 3 (20 participants with chronic periodontitis). Clinical parameters such as gingival index, periodontal index, probing pocket depth, and clinical attachment level were assessed. GCF and blood samples were taken from these patients and assessed for the mRNA expression of ezrin using real-time polymerase chain reaction. RESULTS: The expression and mean relative quantification of mRNA expression of ezrin in GCF and blood were higher for periodontitis (18.32 ± 8.398, 19.34 ± 9.487) when compared to that of gingivitis (5.34 ± 3.609, 5.48 ± 4.428) and healthy individuals (2.33 ± 0.643, 3.47 ± 1.923) and they positively correlated with the clinical parameters. CONCLUSION: The increased expression of ezrin can be considered as a good indicator to assess the inflammatory activity in periodontitis and gingivitis. |
format | Online Article Text |
id | pubmed-9533393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-95333932022-10-06 mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study Cecil, Anju Sambashivaiah, Savita Bilichodmath, Shivaprasad John, Rubin S. Contemp Clin Dent Original Article BACKGROUND: A comparative analysis of protein expression of gingival crevicular fluid (GCF) obtained from healthy individuals and individuals with periodontal diseases would help to identify proteins involved in periodontal disease progression. Among the identified proteins, Moesin which is a disease-associated protein belongs to the ezrin-radixin-moesin protein family and was proved to play an important role in the recognition of oral bacteria contributing to the consequent development of inflammatory immune responses involved in periodontal disease development. AIM: The aim of the study is to quantify and compare mRNA expression levels of ezrin in GCF and whole blood of gingivitis and chronic periodontitis patients. MATERIALS AND METHODS: A total of 60 patients were selected for the study and were divided into three groups as follows: Group 1 (20 participants with healthy gingiva), Group 2 (20 participants with gingivitis), and Group 3 (20 participants with chronic periodontitis). Clinical parameters such as gingival index, periodontal index, probing pocket depth, and clinical attachment level were assessed. GCF and blood samples were taken from these patients and assessed for the mRNA expression of ezrin using real-time polymerase chain reaction. RESULTS: The expression and mean relative quantification of mRNA expression of ezrin in GCF and blood were higher for periodontitis (18.32 ± 8.398, 19.34 ± 9.487) when compared to that of gingivitis (5.34 ± 3.609, 5.48 ± 4.428) and healthy individuals (2.33 ± 0.643, 3.47 ± 1.923) and they positively correlated with the clinical parameters. CONCLUSION: The increased expression of ezrin can be considered as a good indicator to assess the inflammatory activity in periodontitis and gingivitis. Medknow Publications & Media Pvt Ltd 2022 2022-09-24 /pmc/articles/PMC9533393/ /pubmed/36213856 http://dx.doi.org/10.4103/ccd.ccd_6_21 Text en Copyright: © 2022 Contemporary Clinical Dentistry https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Cecil, Anju Sambashivaiah, Savita Bilichodmath, Shivaprasad John, Rubin S. mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study |
title | mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study |
title_full | mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study |
title_fullStr | mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study |
title_full_unstemmed | mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study |
title_short | mRNA Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients – A Polymerase Chain Reaction Study |
title_sort | mrna expression of ezrin in gingival crevicular fluid and whole blood of gingivitis and chronic periodontitis patients – a polymerase chain reaction study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533393/ https://www.ncbi.nlm.nih.gov/pubmed/36213856 http://dx.doi.org/10.4103/ccd.ccd_6_21 |
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