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CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging
The biological state at the subcellular level is highly relevant to many diseases, and the monitoring of organelles such as mitochondria is crucial based on this. However, most DNA and protein based nanoprobes used for the detection of mitochondrial RNAs (mitomiRs) lack spatial selectivity, which le...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533423/ https://www.ncbi.nlm.nih.gov/pubmed/36320584 http://dx.doi.org/10.1039/d2sc03329a |
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author | Zhao, Xuan Na, Na Ouyang, Jin |
author_facet | Zhao, Xuan Na, Na Ouyang, Jin |
author_sort | Zhao, Xuan |
collection | PubMed |
description | The biological state at the subcellular level is highly relevant to many diseases, and the monitoring of organelles such as mitochondria is crucial based on this. However, most DNA and protein based nanoprobes used for the detection of mitochondrial RNAs (mitomiRs) lack spatial selectivity, which leads to inefficiencies in probe delivery and signal turn-on. Herein, we constructed a novel DNA nanoprobe named protein delivery nano-corona (PDNC) to improve the delivery efficiency of Cas protein, for spatially selective imaging of mitomiRs in living cells switched on by a CRISPR/Cas system. Combined with a single-molecule counting method, this strategy enables highly sensitive detection of low-abundance mitomiR. Therefore, the strategy in this work opens up new opportunities for cell identification, early clinical diagnosis, and research in biological behaviour at the subcellular level. |
format | Online Article Text |
id | pubmed-9533423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-95334232022-10-31 CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging Zhao, Xuan Na, Na Ouyang, Jin Chem Sci Chemistry The biological state at the subcellular level is highly relevant to many diseases, and the monitoring of organelles such as mitochondria is crucial based on this. However, most DNA and protein based nanoprobes used for the detection of mitochondrial RNAs (mitomiRs) lack spatial selectivity, which leads to inefficiencies in probe delivery and signal turn-on. Herein, we constructed a novel DNA nanoprobe named protein delivery nano-corona (PDNC) to improve the delivery efficiency of Cas protein, for spatially selective imaging of mitomiRs in living cells switched on by a CRISPR/Cas system. Combined with a single-molecule counting method, this strategy enables highly sensitive detection of low-abundance mitomiR. Therefore, the strategy in this work opens up new opportunities for cell identification, early clinical diagnosis, and research in biological behaviour at the subcellular level. The Royal Society of Chemistry 2022-09-10 /pmc/articles/PMC9533423/ /pubmed/36320584 http://dx.doi.org/10.1039/d2sc03329a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Zhao, Xuan Na, Na Ouyang, Jin CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging |
title | CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging |
title_full | CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging |
title_fullStr | CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging |
title_full_unstemmed | CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging |
title_short | CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging |
title_sort | crispr/cas9-based coronal nanostructures for targeted mitochondria single molecule imaging |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533423/ https://www.ncbi.nlm.nih.gov/pubmed/36320584 http://dx.doi.org/10.1039/d2sc03329a |
work_keys_str_mv | AT zhaoxuan crisprcas9basedcoronalnanostructuresfortargetedmitochondriasinglemoleculeimaging AT nana crisprcas9basedcoronalnanostructuresfortargetedmitochondriasinglemoleculeimaging AT ouyangjin crisprcas9basedcoronalnanostructuresfortargetedmitochondriasinglemoleculeimaging |