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Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato

Sulfur-containing compounds are essential for plant development and environmental adaptation, and closely related to the flavor and nutrition of the agricultural products. Cysteine, the first organic sulfur-containing molecule generated in plants, is the precursor for most of these active substances...

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Autores principales: Liu, Danmei, Li, Min, Guo, Ting, Lu, Juanjuan, Xie, Yafang, Hao, Yuan, Wang, Longdan, Zhao, Dan, Zhang, Liping, Liu, Zhiqiang, Jin, Zhuping, Pei, Yanxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533716/
https://www.ncbi.nlm.nih.gov/pubmed/36212318
http://dx.doi.org/10.3389/fpls.2022.913856
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author Liu, Danmei
Li, Min
Guo, Ting
Lu, Juanjuan
Xie, Yafang
Hao, Yuan
Wang, Longdan
Zhao, Dan
Zhang, Liping
Liu, Zhiqiang
Jin, Zhuping
Pei, Yanxi
author_facet Liu, Danmei
Li, Min
Guo, Ting
Lu, Juanjuan
Xie, Yafang
Hao, Yuan
Wang, Longdan
Zhao, Dan
Zhang, Liping
Liu, Zhiqiang
Jin, Zhuping
Pei, Yanxi
author_sort Liu, Danmei
collection PubMed
description Sulfur-containing compounds are essential for plant development and environmental adaptation, and closely related to the flavor and nutrition of the agricultural products. Cysteine, the first organic sulfur-containing molecule generated in plants, is the precursor for most of these active substances. Serine acetyltransferase (SERAT) catalyzes the rate-limiting step of its formation. However, despite their importance, systematic analyses of these enzymes in individual species, especially in economically important crops, are still limited. Here, The SERAT members (SlSERATs, four in total) were identified and characterized in tomato. Phylogenetically, the four SlSERAT proteins were classified into three subgroups with distinct genomic structures and subcellular localizations. On the function, it was interesting to find that SlSERAT3;1, possessed a high ability to catalyze the formation of OAS, even though it contained a long C-terminus. However, it retained the essential C-terminal Ile, which seems to be a characteristic feature of SERAT3 subfamily members in Solanaceae. Besides, SlSERAT1;1 and SlSERAT2;2 also had high activity levels and their catalyzing abilities were significantly improved by the addition of an OAS-(thiol)-lyase protein. At the transcriptional level, the four SlSERAT genes had distinct expression patterns during tomato plant development. Under abiotic stress conditions, the chloroplast-localized SlSERATs were the main responders, and the SlSERATs adopted different strategies to cope with osmotic, ion toxicity and other stresses. Finally, analyses in the loss-of-function and overexpression lines of SlSERAT1;1 suggested that function redundancy existed in the tomato SERAT members, and the tomato SERAT member was ideal target for S-assimilation manipulating in molecular breeding.
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spelling pubmed-95337162022-10-06 Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato Liu, Danmei Li, Min Guo, Ting Lu, Juanjuan Xie, Yafang Hao, Yuan Wang, Longdan Zhao, Dan Zhang, Liping Liu, Zhiqiang Jin, Zhuping Pei, Yanxi Front Plant Sci Plant Science Sulfur-containing compounds are essential for plant development and environmental adaptation, and closely related to the flavor and nutrition of the agricultural products. Cysteine, the first organic sulfur-containing molecule generated in plants, is the precursor for most of these active substances. Serine acetyltransferase (SERAT) catalyzes the rate-limiting step of its formation. However, despite their importance, systematic analyses of these enzymes in individual species, especially in economically important crops, are still limited. Here, The SERAT members (SlSERATs, four in total) were identified and characterized in tomato. Phylogenetically, the four SlSERAT proteins were classified into three subgroups with distinct genomic structures and subcellular localizations. On the function, it was interesting to find that SlSERAT3;1, possessed a high ability to catalyze the formation of OAS, even though it contained a long C-terminus. However, it retained the essential C-terminal Ile, which seems to be a characteristic feature of SERAT3 subfamily members in Solanaceae. Besides, SlSERAT1;1 and SlSERAT2;2 also had high activity levels and their catalyzing abilities were significantly improved by the addition of an OAS-(thiol)-lyase protein. At the transcriptional level, the four SlSERAT genes had distinct expression patterns during tomato plant development. Under abiotic stress conditions, the chloroplast-localized SlSERATs were the main responders, and the SlSERATs adopted different strategies to cope with osmotic, ion toxicity and other stresses. Finally, analyses in the loss-of-function and overexpression lines of SlSERAT1;1 suggested that function redundancy existed in the tomato SERAT members, and the tomato SERAT member was ideal target for S-assimilation manipulating in molecular breeding. Frontiers Media S.A. 2022-09-21 /pmc/articles/PMC9533716/ /pubmed/36212318 http://dx.doi.org/10.3389/fpls.2022.913856 Text en Copyright © 2022 Liu, Li, Guo, Lu, Xie, Hao, Wang, Zhao, Zhang, Liu, Jin and Pei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Danmei
Li, Min
Guo, Ting
Lu, Juanjuan
Xie, Yafang
Hao, Yuan
Wang, Longdan
Zhao, Dan
Zhang, Liping
Liu, Zhiqiang
Jin, Zhuping
Pei, Yanxi
Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
title Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
title_full Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
title_fullStr Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
title_full_unstemmed Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
title_short Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
title_sort functional characterization of the serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533716/
https://www.ncbi.nlm.nih.gov/pubmed/36212318
http://dx.doi.org/10.3389/fpls.2022.913856
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