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Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae

Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling soil-borne diseases. Verticillium dahliae, a notorious fungal pathogen, causes economically important wilt diseases in agriculture and forestry industries. Here, we de...

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Autores principales: Kong, Wei-Liang, Ni, Hang, Wang, Wei-Yu, Wu, Xiao-Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533717/
https://www.ncbi.nlm.nih.gov/pubmed/36212874
http://dx.doi.org/10.3389/fmicb.2022.1013468
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author Kong, Wei-Liang
Ni, Hang
Wang, Wei-Yu
Wu, Xiao-Qin
author_facet Kong, Wei-Liang
Ni, Hang
Wang, Wei-Yu
Wu, Xiao-Qin
author_sort Kong, Wei-Liang
collection PubMed
description Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling soil-borne diseases. Verticillium dahliae, a notorious fungal pathogen, causes economically important wilt diseases in agriculture and forestry industries. Here, we determined the antifungal activity of VOCs produced by Trichoderma koningiopsis T2. The VOCs from T. koningiopsis T2 were trapped by solid-phase microextraction (SPME) and tentatively identified through gas chromatography–mass spectrometry (GC/MS). The microsclerotia formation, cell wall-degrading enzymes and melanin synthesis of V. dahliae exposed to the VOC mixtures and selected single standards were examined. The results showed that the VOCs produced by strain T2 significantly inhibited the growth of V. dahliae mycelium and reduced the severity of Verticillium wilt in tobacco and cotton. Six individual compounds were identified in the volatilome of T. koningiopsis T2, and the dominant compounds were 3-octanone, 3-methyl-1-butanol, butanoic acid ethyl ester and 2-hexyl-furan. The VOCs of strain T2 exert a significant inhibitory effect on microsclerotia formation and decreased the activities of pectin lyase and endo-β-1,4-glucanase in V. dahliae. VOCs also downregulated the VdT3HR, VdT4HR, and VdSCD genes related to melanin synthesis by 29. 41-, 10. 49-, and 3.11-fold, respectively. Therefore, T. koningiopsis T2 has potential as a promising biofumigant for the biocontrol of Verticillium wilt disease.
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spelling pubmed-95337172022-10-06 Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae Kong, Wei-Liang Ni, Hang Wang, Wei-Yu Wu, Xiao-Qin Front Microbiol Microbiology Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling soil-borne diseases. Verticillium dahliae, a notorious fungal pathogen, causes economically important wilt diseases in agriculture and forestry industries. Here, we determined the antifungal activity of VOCs produced by Trichoderma koningiopsis T2. The VOCs from T. koningiopsis T2 were trapped by solid-phase microextraction (SPME) and tentatively identified through gas chromatography–mass spectrometry (GC/MS). The microsclerotia formation, cell wall-degrading enzymes and melanin synthesis of V. dahliae exposed to the VOC mixtures and selected single standards were examined. The results showed that the VOCs produced by strain T2 significantly inhibited the growth of V. dahliae mycelium and reduced the severity of Verticillium wilt in tobacco and cotton. Six individual compounds were identified in the volatilome of T. koningiopsis T2, and the dominant compounds were 3-octanone, 3-methyl-1-butanol, butanoic acid ethyl ester and 2-hexyl-furan. The VOCs of strain T2 exert a significant inhibitory effect on microsclerotia formation and decreased the activities of pectin lyase and endo-β-1,4-glucanase in V. dahliae. VOCs also downregulated the VdT3HR, VdT4HR, and VdSCD genes related to melanin synthesis by 29. 41-, 10. 49-, and 3.11-fold, respectively. Therefore, T. koningiopsis T2 has potential as a promising biofumigant for the biocontrol of Verticillium wilt disease. Frontiers Media S.A. 2022-09-21 /pmc/articles/PMC9533717/ /pubmed/36212874 http://dx.doi.org/10.3389/fmicb.2022.1013468 Text en Copyright © 2022 Kong, Ni, Wang and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kong, Wei-Liang
Ni, Hang
Wang, Wei-Yu
Wu, Xiao-Qin
Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae
title Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae
title_full Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae
title_fullStr Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae
title_full_unstemmed Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae
title_short Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae
title_sort antifungal effects of volatile organic compounds produced by trichoderma koningiopsis t2 against verticillium dahliae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533717/
https://www.ncbi.nlm.nih.gov/pubmed/36212874
http://dx.doi.org/10.3389/fmicb.2022.1013468
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