Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans

A method for one-stage rapid identification of four orthopoxvirus species pathogenic to humans based on multiplex polymerase chain reaction (MPCR) was developed. Five pairs of oligonucleotide primers—one, genus-specific; and the rest, species-specific for variola, monkeypox, cowpox, and vaccinia vir...

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Detalles Bibliográficos
Autores principales: Shchelkunov, S.N., Gavrilova, E.V., Babkin, I.V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533918/
https://www.ncbi.nlm.nih.gov/pubmed/15652214
http://dx.doi.org/10.1016/j.mcp.2004.07.004
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author Shchelkunov, S.N.
Gavrilova, E.V.
Babkin, I.V.
author_facet Shchelkunov, S.N.
Gavrilova, E.V.
Babkin, I.V.
author_sort Shchelkunov, S.N.
collection PubMed
description A method for one-stage rapid identification of four orthopoxvirus species pathogenic to humans based on multiplex polymerase chain reaction (MPCR) was developed. Five pairs of oligonucleotide primers—one, genus-specific; and the rest, species-specific for variola, monkeypox, cowpox, and vaccinia viruses, respectively—were used concurrently for MPCR assay of orthopoxvirus DNAs. Specificity and sensitivity of the method developed were evaluated using DNAs of 57 orthopoxvirus strains, including the DNAs isolated from human case clinical materials.
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spelling pubmed-95339182022-10-07 Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans Shchelkunov, S.N. Gavrilova, E.V. Babkin, I.V. Mol Cell Probes Article A method for one-stage rapid identification of four orthopoxvirus species pathogenic to humans based on multiplex polymerase chain reaction (MPCR) was developed. Five pairs of oligonucleotide primers—one, genus-specific; and the rest, species-specific for variola, monkeypox, cowpox, and vaccinia viruses, respectively—were used concurrently for MPCR assay of orthopoxvirus DNAs. Specificity and sensitivity of the method developed were evaluated using DNAs of 57 orthopoxvirus strains, including the DNAs isolated from human case clinical materials. Elsevier Ltd. 2005-02 2004-10-18 /pmc/articles/PMC9533918/ /pubmed/15652214 http://dx.doi.org/10.1016/j.mcp.2004.07.004 Text en Copyright © 2004 Elsevier Ltd. All rights reserved. Elsevier has created a Monkeypox Information Center in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.
spellingShingle Article
Shchelkunov, S.N.
Gavrilova, E.V.
Babkin, I.V.
Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans
title Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans
title_full Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans
title_fullStr Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans
title_full_unstemmed Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans
title_short Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans
title_sort multiplex pcr detection and species differentiation of orthopoxviruses pathogenic to humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9533918/
https://www.ncbi.nlm.nih.gov/pubmed/15652214
http://dx.doi.org/10.1016/j.mcp.2004.07.004
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