Cargando…

Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens

The commercially available Orthopox BioThreat(®) Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to...

Descripción completa

Detalles Bibliográficos
Autores principales: Townsend, Michael B., MacNeil, Adam, Reynolds, Mary G., Hughes, Christine M., Olson, Victoria A., Damon, Inger K., Karem, Kevin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. Published by Elsevier B.V. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534008/
https://www.ncbi.nlm.nih.gov/pubmed/22981983
http://dx.doi.org/10.1016/j.jviromet.2012.08.023
_version_ 1784802455258660864
author Townsend, Michael B.
MacNeil, Adam
Reynolds, Mary G.
Hughes, Christine M.
Olson, Victoria A.
Damon, Inger K.
Karem, Kevin L.
author_facet Townsend, Michael B.
MacNeil, Adam
Reynolds, Mary G.
Hughes, Christine M.
Olson, Victoria A.
Damon, Inger K.
Karem, Kevin L.
author_sort Townsend, Michael B.
collection PubMed
description The commercially available Orthopox BioThreat(®) Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to evaluate the sensitivity of the Tetracore assay by visual and quantitative determinations; specificity was assessed using a small but diverse set of diagnostically relevant blinded samples from viral lesions submitted for routine OPV diagnostic testing. The BioThreat(®) Alert assay reproducibly detected samples at concentrations of 10(7) pfu/ml for VACV and MPXV and positively identified samples containing 10(6) pfu/ml in 4 of 7 independent experiments. The assay correctly identified 9 of 11 OPV clinical samples and had only one false positive when testing 11 non-OPV samples. Results suggest applicability for use of the BioThreat(®) Alert assay as a rapid screening assay and point of care diagnosis for suspect human monkeypox cases.
format Online
Article
Text
id pubmed-9534008
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Elsevier B.V. Published by Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-95340082022-10-07 Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens Townsend, Michael B. MacNeil, Adam Reynolds, Mary G. Hughes, Christine M. Olson, Victoria A. Damon, Inger K. Karem, Kevin L. J Virol Methods Article The commercially available Orthopox BioThreat(®) Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to evaluate the sensitivity of the Tetracore assay by visual and quantitative determinations; specificity was assessed using a small but diverse set of diagnostically relevant blinded samples from viral lesions submitted for routine OPV diagnostic testing. The BioThreat(®) Alert assay reproducibly detected samples at concentrations of 10(7) pfu/ml for VACV and MPXV and positively identified samples containing 10(6) pfu/ml in 4 of 7 independent experiments. The assay correctly identified 9 of 11 OPV clinical samples and had only one false positive when testing 11 non-OPV samples. Results suggest applicability for use of the BioThreat(®) Alert assay as a rapid screening assay and point of care diagnosis for suspect human monkeypox cases. Elsevier B.V. Published by Elsevier B.V. 2013-01 2012-09-04 /pmc/articles/PMC9534008/ /pubmed/22981983 http://dx.doi.org/10.1016/j.jviromet.2012.08.023 Text en Copyright © 2012 Elsevier B.V. Published by Elsevier B.V. All rights reserved. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.
spellingShingle Article
Townsend, Michael B.
MacNeil, Adam
Reynolds, Mary G.
Hughes, Christine M.
Olson, Victoria A.
Damon, Inger K.
Karem, Kevin L.
Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
title Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
title_full Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
title_fullStr Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
title_full_unstemmed Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
title_short Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
title_sort evaluation of the tetracore orthopox biothreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534008/
https://www.ncbi.nlm.nih.gov/pubmed/22981983
http://dx.doi.org/10.1016/j.jviromet.2012.08.023
work_keys_str_mv AT townsendmichaelb evaluationofthetetracoreorthopoxbiothreatantigendetectionassayusinglaboratorygrownorthopoxvirusesandrashillnessclinicalspecimens
AT macneiladam evaluationofthetetracoreorthopoxbiothreatantigendetectionassayusinglaboratorygrownorthopoxvirusesandrashillnessclinicalspecimens
AT reynoldsmaryg evaluationofthetetracoreorthopoxbiothreatantigendetectionassayusinglaboratorygrownorthopoxvirusesandrashillnessclinicalspecimens
AT hugheschristinem evaluationofthetetracoreorthopoxbiothreatantigendetectionassayusinglaboratorygrownorthopoxvirusesandrashillnessclinicalspecimens
AT olsonvictoriaa evaluationofthetetracoreorthopoxbiothreatantigendetectionassayusinglaboratorygrownorthopoxvirusesandrashillnessclinicalspecimens
AT damoningerk evaluationofthetetracoreorthopoxbiothreatantigendetectionassayusinglaboratorygrownorthopoxvirusesandrashillnessclinicalspecimens
AT karemkevinl evaluationofthetetracoreorthopoxbiothreatantigendetectionassayusinglaboratorygrownorthopoxvirusesandrashillnessclinicalspecimens