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Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens
The commercially available Orthopox BioThreat(®) Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V. Published by Elsevier B.V.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534008/ https://www.ncbi.nlm.nih.gov/pubmed/22981983 http://dx.doi.org/10.1016/j.jviromet.2012.08.023 |
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author | Townsend, Michael B. MacNeil, Adam Reynolds, Mary G. Hughes, Christine M. Olson, Victoria A. Damon, Inger K. Karem, Kevin L. |
author_facet | Townsend, Michael B. MacNeil, Adam Reynolds, Mary G. Hughes, Christine M. Olson, Victoria A. Damon, Inger K. Karem, Kevin L. |
author_sort | Townsend, Michael B. |
collection | PubMed |
description | The commercially available Orthopox BioThreat(®) Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to evaluate the sensitivity of the Tetracore assay by visual and quantitative determinations; specificity was assessed using a small but diverse set of diagnostically relevant blinded samples from viral lesions submitted for routine OPV diagnostic testing. The BioThreat(®) Alert assay reproducibly detected samples at concentrations of 10(7) pfu/ml for VACV and MPXV and positively identified samples containing 10(6) pfu/ml in 4 of 7 independent experiments. The assay correctly identified 9 of 11 OPV clinical samples and had only one false positive when testing 11 non-OPV samples. Results suggest applicability for use of the BioThreat(®) Alert assay as a rapid screening assay and point of care diagnosis for suspect human monkeypox cases. |
format | Online Article Text |
id | pubmed-9534008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier B.V. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95340082022-10-07 Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens Townsend, Michael B. MacNeil, Adam Reynolds, Mary G. Hughes, Christine M. Olson, Victoria A. Damon, Inger K. Karem, Kevin L. J Virol Methods Article The commercially available Orthopox BioThreat(®) Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to evaluate the sensitivity of the Tetracore assay by visual and quantitative determinations; specificity was assessed using a small but diverse set of diagnostically relevant blinded samples from viral lesions submitted for routine OPV diagnostic testing. The BioThreat(®) Alert assay reproducibly detected samples at concentrations of 10(7) pfu/ml for VACV and MPXV and positively identified samples containing 10(6) pfu/ml in 4 of 7 independent experiments. The assay correctly identified 9 of 11 OPV clinical samples and had only one false positive when testing 11 non-OPV samples. Results suggest applicability for use of the BioThreat(®) Alert assay as a rapid screening assay and point of care diagnosis for suspect human monkeypox cases. Elsevier B.V. Published by Elsevier B.V. 2013-01 2012-09-04 /pmc/articles/PMC9534008/ /pubmed/22981983 http://dx.doi.org/10.1016/j.jviromet.2012.08.023 Text en Copyright © 2012 Elsevier B.V. Published by Elsevier B.V. All rights reserved. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active. |
spellingShingle | Article Townsend, Michael B. MacNeil, Adam Reynolds, Mary G. Hughes, Christine M. Olson, Victoria A. Damon, Inger K. Karem, Kevin L. Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens |
title | Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens |
title_full | Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens |
title_fullStr | Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens |
title_full_unstemmed | Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens |
title_short | Evaluation of the Tetracore Orthopox BioThreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens |
title_sort | evaluation of the tetracore orthopox biothreat(®) antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534008/ https://www.ncbi.nlm.nih.gov/pubmed/22981983 http://dx.doi.org/10.1016/j.jviromet.2012.08.023 |
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