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Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis

Arabidopsis thaliana is one of the most studied model organisms of plant biology with hundreds of geographical variants called ecotypes. One might expect that this enormous genetic variety could result in differential response to pathogens. Indeed, we observed previously that the Bur ecotype develop...

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Autores principales: Gyula, Péter, Tóth, Tamás, Gorcsa, Teréz, Nyikó, Tünde, Sós-Hegedűs, Anita, Szittya, György
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534422/
https://www.ncbi.nlm.nih.gov/pubmed/36197942
http://dx.doi.org/10.1371/journal.pone.0275588
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author Gyula, Péter
Tóth, Tamás
Gorcsa, Teréz
Nyikó, Tünde
Sós-Hegedűs, Anita
Szittya, György
author_facet Gyula, Péter
Tóth, Tamás
Gorcsa, Teréz
Nyikó, Tünde
Sós-Hegedűs, Anita
Szittya, György
author_sort Gyula, Péter
collection PubMed
description Arabidopsis thaliana is one of the most studied model organisms of plant biology with hundreds of geographical variants called ecotypes. One might expect that this enormous genetic variety could result in differential response to pathogens. Indeed, we observed previously that the Bur ecotype develops much more severe symptoms (upward curling leaves and wavy leaf margins) upon infection with two positive-strand RNA viruses of different families (turnip vein-clearing virus, TVCV, and turnip mosaic virus, TuMV). To find the genes potentially responsible for the ecotype-specific response, we performed a differential expression analysis of the mRNA and sRNA pools of TVCV and TuMV-infected Bur and Col plants along with the corresponding mock controls. We focused on the genes and sRNAs that showed an induced or reduced expression selectively in the Bur virus samples in both virus series. We found that the two ecotypes respond to the viral infection differently, yet both viruses selectively block the production of the TAS3-derived small RNA specimen called tasiARF only in the virus-infected Bur plants. The tasiARF normally forms a gradient through the adaxial and abaxial parts of the leaf (being more abundant in the adaxial part) and post-transcriptionally regulates ARF4, a major leaf polarity determinant in plants. The lack of tasiARF-mediated silencing could lead to an ectopically expressed ARF4 in the adaxial part of the leaf where the misregulation of auxin-dependent signaling would result in an irregular growth of the leaf blade manifesting as upward curling leaf and wavy leaf margin. QTL mapping using Recombinant Inbred Lines (RILs) suggests that the observed symptoms are the result of a multigenic interaction that allows the symptoms to develop only in the Bur ecotype. The particular nature of genetic differences leading to the ecotype-specific symptoms remains obscure and needs further study.
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spelling pubmed-95344222022-10-06 Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis Gyula, Péter Tóth, Tamás Gorcsa, Teréz Nyikó, Tünde Sós-Hegedűs, Anita Szittya, György PLoS One Research Article Arabidopsis thaliana is one of the most studied model organisms of plant biology with hundreds of geographical variants called ecotypes. One might expect that this enormous genetic variety could result in differential response to pathogens. Indeed, we observed previously that the Bur ecotype develops much more severe symptoms (upward curling leaves and wavy leaf margins) upon infection with two positive-strand RNA viruses of different families (turnip vein-clearing virus, TVCV, and turnip mosaic virus, TuMV). To find the genes potentially responsible for the ecotype-specific response, we performed a differential expression analysis of the mRNA and sRNA pools of TVCV and TuMV-infected Bur and Col plants along with the corresponding mock controls. We focused on the genes and sRNAs that showed an induced or reduced expression selectively in the Bur virus samples in both virus series. We found that the two ecotypes respond to the viral infection differently, yet both viruses selectively block the production of the TAS3-derived small RNA specimen called tasiARF only in the virus-infected Bur plants. The tasiARF normally forms a gradient through the adaxial and abaxial parts of the leaf (being more abundant in the adaxial part) and post-transcriptionally regulates ARF4, a major leaf polarity determinant in plants. The lack of tasiARF-mediated silencing could lead to an ectopically expressed ARF4 in the adaxial part of the leaf where the misregulation of auxin-dependent signaling would result in an irregular growth of the leaf blade manifesting as upward curling leaf and wavy leaf margin. QTL mapping using Recombinant Inbred Lines (RILs) suggests that the observed symptoms are the result of a multigenic interaction that allows the symptoms to develop only in the Bur ecotype. The particular nature of genetic differences leading to the ecotype-specific symptoms remains obscure and needs further study. Public Library of Science 2022-10-05 /pmc/articles/PMC9534422/ /pubmed/36197942 http://dx.doi.org/10.1371/journal.pone.0275588 Text en © 2022 Gyula et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gyula, Péter
Tóth, Tamás
Gorcsa, Teréz
Nyikó, Tünde
Sós-Hegedűs, Anita
Szittya, György
Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis
title Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis
title_full Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis
title_fullStr Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis
title_full_unstemmed Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis
title_short Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis
title_sort ecotype-specific blockage of tasiarf production by two different rna viruses in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534422/
https://www.ncbi.nlm.nih.gov/pubmed/36197942
http://dx.doi.org/10.1371/journal.pone.0275588
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