The inhibitory activity of methoxyl flavonoids derived from Inula britannica flowers on SARS-CoV-2 3CLpro

In our ongoing efforts to identify effective natural antiviral agents, four methoxy flavonoids (1–4) were isolated from the Inula britannica flower extract. Their structures were elucidated using nuclear magnetic resonance. Flavonoids 1–4 exhibited inhibitory activity against SARS- CoV-2 3CLpro with...

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Detalles Bibliográficos
Autores principales: Kim, Jang Hoon, Park, Yea-In, Hur, Mok, Park, Woo Tae, Moon, Youn-Ho, Koo, Sung Cheol, Yun-Chan, Her, Lee, Ik Soo, Park, Junsoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534544/
https://www.ncbi.nlm.nih.gov/pubmed/36208809
http://dx.doi.org/10.1016/j.ijbiomac.2022.10.008
Descripción
Sumario:In our ongoing efforts to identify effective natural antiviral agents, four methoxy flavonoids (1–4) were isolated from the Inula britannica flower extract. Their structures were elucidated using nuclear magnetic resonance. Flavonoids 1–4 exhibited inhibitory activity against SARS- CoV-2 3CLpro with IC(50) values of 41.6 ± 2.5, 35.9 ± 0.9, 32.8 ± 1.2, and 96.6 ± 3.4 μM, respectively. Flavonoids 1–3 inhibited 3CLpro in a competitive manner. Based on molecular simulations, key amino acids that form hydrogen bond with inhibitor 3 were identified. Finally, we found that inhibitors (1–3) suppressed HCoV-OC43 coronavirus proliferation at micromole concentrations.

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