Cargando…

Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L

Rhizosphere bacterial diversity and community structure are important factors involving in plant growth. However, the exact process of how plant rhizosphere bacterial community structures is assembled remains unclear. To investigate the role of bacterial chemotaxis to rhizosphere secretions in the e...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Qi, Li, Miaomiao, Wang, Ying, Xu, Zhixia, Li, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534574/
https://www.ncbi.nlm.nih.gov/pubmed/36212345
http://dx.doi.org/10.3389/fpls.2022.988442
_version_ 1784802573464633344
author Lin, Qi
Li, Miaomiao
Wang, Ying
Xu, Zhixia
Li, Lei
author_facet Lin, Qi
Li, Miaomiao
Wang, Ying
Xu, Zhixia
Li, Lei
author_sort Lin, Qi
collection PubMed
description Rhizosphere bacterial diversity and community structure are important factors involving in plant growth. However, the exact process of how plant rhizosphere bacterial community structures is assembled remains unclear. To investigate the role of bacterial chemotaxis to rhizosphere secretions in the establishment of rhizosphere microbial community in Casuarina equisetifolia, we screened bacteria strains derived from the rhizosphere of Casuarina equisetifolia L. using top three chemicals of the plant root exudates (2,4-di-tert-butylphenol, methyl stearate, and arginine) as chemoattractant. Among 72 bacterial strains, five showed strong chemotaxis to 2,4-di-tert-butylphenol, six to methyl stearate, and eleven to arginine, with the highest bacterial chemotaxis occurring at a concentration of 60 μM. This indicates that arginine is a more important chemoattractant than 2,4-di-tert-butylphenol, methyl stearate in the establishment of rhizosphere microbial community in Casuarina equisetifolia. Bacterial community assembly analysis using different chemoattractants and chemoattractants-plus-bacteria combinations were then performed by burying laboratory prepared bags of sterlized soil into C. equisetifolia forest. Bacteria diversity and enrichment analyses using 16S rDNA sequencing at 7 and 14 days after burying showed that arginine-plus-Ochrobactrum sp. and Pantoea sp. treatment exhibited the greatest similarity to the natural forest bacterial community. Our date provides new insights into how chemoattractants and chemotactic bacteria strains shape the rhizosphere microbial community of C. equisetifolia, which constitutes foundational information for future management of these communities.
format Online
Article
Text
id pubmed-9534574
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95345742022-10-06 Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L Lin, Qi Li, Miaomiao Wang, Ying Xu, Zhixia Li, Lei Front Plant Sci Plant Science Rhizosphere bacterial diversity and community structure are important factors involving in plant growth. However, the exact process of how plant rhizosphere bacterial community structures is assembled remains unclear. To investigate the role of bacterial chemotaxis to rhizosphere secretions in the establishment of rhizosphere microbial community in Casuarina equisetifolia, we screened bacteria strains derived from the rhizosphere of Casuarina equisetifolia L. using top three chemicals of the plant root exudates (2,4-di-tert-butylphenol, methyl stearate, and arginine) as chemoattractant. Among 72 bacterial strains, five showed strong chemotaxis to 2,4-di-tert-butylphenol, six to methyl stearate, and eleven to arginine, with the highest bacterial chemotaxis occurring at a concentration of 60 μM. This indicates that arginine is a more important chemoattractant than 2,4-di-tert-butylphenol, methyl stearate in the establishment of rhizosphere microbial community in Casuarina equisetifolia. Bacterial community assembly analysis using different chemoattractants and chemoattractants-plus-bacteria combinations were then performed by burying laboratory prepared bags of sterlized soil into C. equisetifolia forest. Bacteria diversity and enrichment analyses using 16S rDNA sequencing at 7 and 14 days after burying showed that arginine-plus-Ochrobactrum sp. and Pantoea sp. treatment exhibited the greatest similarity to the natural forest bacterial community. Our date provides new insights into how chemoattractants and chemotactic bacteria strains shape the rhizosphere microbial community of C. equisetifolia, which constitutes foundational information for future management of these communities. Frontiers Media S.A. 2022-09-20 /pmc/articles/PMC9534574/ /pubmed/36212345 http://dx.doi.org/10.3389/fpls.2022.988442 Text en Copyright © 2022 Lin, Li, Wang, Xu and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lin, Qi
Li, Miaomiao
Wang, Ying
Xu, Zhixia
Li, Lei
Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L
title Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L
title_full Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L
title_fullStr Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L
title_full_unstemmed Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L
title_short Root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of Casuarina equisetifolia L
title_sort root exudates and chemotactic strains mediate bacterial community assembly in the rhizosphere soil of casuarina equisetifolia l
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534574/
https://www.ncbi.nlm.nih.gov/pubmed/36212345
http://dx.doi.org/10.3389/fpls.2022.988442
work_keys_str_mv AT linqi rootexudatesandchemotacticstrainsmediatebacterialcommunityassemblyintherhizospheresoilofcasuarinaequisetifolial
AT limiaomiao rootexudatesandchemotacticstrainsmediatebacterialcommunityassemblyintherhizospheresoilofcasuarinaequisetifolial
AT wangying rootexudatesandchemotacticstrainsmediatebacterialcommunityassemblyintherhizospheresoilofcasuarinaequisetifolial
AT xuzhixia rootexudatesandchemotacticstrainsmediatebacterialcommunityassemblyintherhizospheresoilofcasuarinaequisetifolial
AT lilei rootexudatesandchemotacticstrainsmediatebacterialcommunityassemblyintherhizospheresoilofcasuarinaequisetifolial