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COVID-19 Whole-Genome Resequencing with Redundant Tiling PCR and Subtract-Based Amplicon Normalization Successfully Characterized SARS-CoV-2 Variants in Clinical Specimens

With an increasing number of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) sequences gathered worldwide, we recognize that deletion mutants and nucleotide substitutions that may affect whole-genome sequencing are accumulating. Here, we propose an additional strategy for tiling PCR for...

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Detalles Bibliográficos
Autores principales: Sugi, Tatsuki, Rahman, Mizanur, Rahim, Rummana, Hasan, Abu, Kawai, Naoko, Hayashida, Kyoko, Yamagishi, Junya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534710/
https://www.ncbi.nlm.nih.gov/pubmed/36212105
http://dx.doi.org/10.1155/2022/2109641