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Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles

Membrane vesicles (MVs) are spherical particles with nanoscale dimensions and characterized by the presence of diverse cargos, such as nucleic acids, proteins, lipids, and cellular metabolites. Many examples of (micro)organisms producing MVs are reported in literature. Among them, bacterial MVs are...

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Autores principales: Faddetta, Teresa, Vassallo, Alberto, Del Duca, Sara, Gallo, Giuseppe, Fani, Renato, Puglia, Anna Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534924/
https://www.ncbi.nlm.nih.gov/pubmed/36198712
http://dx.doi.org/10.1038/s41598-022-21002-z
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author Faddetta, Teresa
Vassallo, Alberto
Del Duca, Sara
Gallo, Giuseppe
Fani, Renato
Puglia, Anna Maria
author_facet Faddetta, Teresa
Vassallo, Alberto
Del Duca, Sara
Gallo, Giuseppe
Fani, Renato
Puglia, Anna Maria
author_sort Faddetta, Teresa
collection PubMed
description Membrane vesicles (MVs) are spherical particles with nanoscale dimensions and characterized by the presence of diverse cargos, such as nucleic acids, proteins, lipids, and cellular metabolites. Many examples of (micro)organisms producing MVs are reported in literature. Among them, bacterial MVs are of particular interest because they are now considered as the fourth mechanism of horizontal gene transfer. Streptomyces bacteria are well-known for their ecological roles and ability to synthesize bioactive compounds, with Streptomyces coelicolor being the model organism. It was previously demonstrated that it can produce distinct populations of MVs characterized by different protein and metabolite cargos. In this work we demonstrated for the first time that MVs of S. coelicolor carry both DNA and RNA and that their DNA content represents the entire chromosome of the bacterium. These findings suggest that MV DNA could have a role in the evolution of Streptomyces genomes and that MVs could be exploited in new strain engineering strategies.
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spelling pubmed-95349242022-10-07 Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles Faddetta, Teresa Vassallo, Alberto Del Duca, Sara Gallo, Giuseppe Fani, Renato Puglia, Anna Maria Sci Rep Article Membrane vesicles (MVs) are spherical particles with nanoscale dimensions and characterized by the presence of diverse cargos, such as nucleic acids, proteins, lipids, and cellular metabolites. Many examples of (micro)organisms producing MVs are reported in literature. Among them, bacterial MVs are of particular interest because they are now considered as the fourth mechanism of horizontal gene transfer. Streptomyces bacteria are well-known for their ecological roles and ability to synthesize bioactive compounds, with Streptomyces coelicolor being the model organism. It was previously demonstrated that it can produce distinct populations of MVs characterized by different protein and metabolite cargos. In this work we demonstrated for the first time that MVs of S. coelicolor carry both DNA and RNA and that their DNA content represents the entire chromosome of the bacterium. These findings suggest that MV DNA could have a role in the evolution of Streptomyces genomes and that MVs could be exploited in new strain engineering strategies. Nature Publishing Group UK 2022-10-05 /pmc/articles/PMC9534924/ /pubmed/36198712 http://dx.doi.org/10.1038/s41598-022-21002-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Faddetta, Teresa
Vassallo, Alberto
Del Duca, Sara
Gallo, Giuseppe
Fani, Renato
Puglia, Anna Maria
Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles
title Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles
title_full Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles
title_fullStr Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles
title_full_unstemmed Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles
title_short Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles
title_sort unravelling the dna sequences carried by streptomyces coelicolor membrane vesicles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534924/
https://www.ncbi.nlm.nih.gov/pubmed/36198712
http://dx.doi.org/10.1038/s41598-022-21002-z
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