Cargando…
Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions
INTRODUCTION: Undernutrition is an underlying cause of mortality in children under five (CU5) years of age. Animal-source foods have been shown to decrease malnutrition in CU5. Livestock are important reservoirs for Campylobacter bacteria, which are recognised as risk factors for child malnutrition....
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535169/ https://www.ncbi.nlm.nih.gov/pubmed/36198455 http://dx.doi.org/10.1136/bmjopen-2022-061311 |
_version_ | 1784802707805044736 |
---|---|
author | Havelaar, Arie H Brhane, Mussie Ahmed, Ibsa Abdusemed Kedir, Jafer Chen, Dehao Deblais, Loic French, Nigel Gebreyes, Wondwossen A Hassen, Jemal Yousuf Li, Xiaolong Manary, Mark J Mekuria, Zelealem Ibrahim, Abdulmuen Mohammed Mummed, Bahar Ojeda, Amanda Rajashekara, Gireesh Roba, Kedir Teji Saleem, Cyrus Singh, Nitya Usmane, Ibsa Aliyi Yang, Yang Yimer, Getnet McKune, Sarah |
author_facet | Havelaar, Arie H Brhane, Mussie Ahmed, Ibsa Abdusemed Kedir, Jafer Chen, Dehao Deblais, Loic French, Nigel Gebreyes, Wondwossen A Hassen, Jemal Yousuf Li, Xiaolong Manary, Mark J Mekuria, Zelealem Ibrahim, Abdulmuen Mohammed Mummed, Bahar Ojeda, Amanda Rajashekara, Gireesh Roba, Kedir Teji Saleem, Cyrus Singh, Nitya Usmane, Ibsa Aliyi Yang, Yang Yimer, Getnet McKune, Sarah |
author_sort | Havelaar, Arie H |
collection | PubMed |
description | INTRODUCTION: Undernutrition is an underlying cause of mortality in children under five (CU5) years of age. Animal-source foods have been shown to decrease malnutrition in CU5. Livestock are important reservoirs for Campylobacter bacteria, which are recognised as risk factors for child malnutrition. Increasing livestock production may be beneficial for improving nutrition of children but these benefits may be negated by increased exposure to Campylobacter and research is needed to evaluate the complex pathways of Campylobacter exposure and infection applicable to low-income and middle-income countries. We aim to identify reservoirs of infection with Campylobacter spp. of infants in rural Eastern Ethiopia and evaluate interactions with child health (environmental enteric dysfunction and stunting) in the context of their sociodemographic environment. METHODS AND ANALYSIS: This longitudinal study involves 115 infants who are followed from birth to 12 months of age and are selected randomly from 10 kebeles of Haramaya woreda, East Hararghe zone, Oromia region, Ethiopia. Questionnaire-based information is obtained on demographics, livelihoods, wealth, health, nutrition and women empowerment; animal ownership/management and diseases; and water, sanitation and hygiene. Faecal samples are collected from infants, mothers, siblings and livestock, drinking water and soil. These samples are analysed by a range of phenotypic and genotypic microbiological methods to characterise the genetic structure of the Campylobacter population in each of these reservoirs, which will support inference about the main sources of exposure for infants. ETHICS AND DISSEMINATION: Ethical approval was obtained from the University of Florida Internal Review Board (IRB201903141), the Haramaya University Institutional Health Research Ethics Committee (COHMS/1010/3796/20) and the Ethiopia National Research Ethics Review Committee (SM/14.1/1059/20). Written informed consent is obtained from all participating households. Research findings will be disseminated to stakeholders through conferences and peer-reviewed journals and through the Feed the Future Innovation Lab for Livestock Systems. |
format | Online Article Text |
id | pubmed-9535169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-95351692022-10-07 Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions Havelaar, Arie H Brhane, Mussie Ahmed, Ibsa Abdusemed Kedir, Jafer Chen, Dehao Deblais, Loic French, Nigel Gebreyes, Wondwossen A Hassen, Jemal Yousuf Li, Xiaolong Manary, Mark J Mekuria, Zelealem Ibrahim, Abdulmuen Mohammed Mummed, Bahar Ojeda, Amanda Rajashekara, Gireesh Roba, Kedir Teji Saleem, Cyrus Singh, Nitya Usmane, Ibsa Aliyi Yang, Yang Yimer, Getnet McKune, Sarah BMJ Open Global Health INTRODUCTION: Undernutrition is an underlying cause of mortality in children under five (CU5) years of age. Animal-source foods have been shown to decrease malnutrition in CU5. Livestock are important reservoirs for Campylobacter bacteria, which are recognised as risk factors for child malnutrition. Increasing livestock production may be beneficial for improving nutrition of children but these benefits may be negated by increased exposure to Campylobacter and research is needed to evaluate the complex pathways of Campylobacter exposure and infection applicable to low-income and middle-income countries. We aim to identify reservoirs of infection with Campylobacter spp. of infants in rural Eastern Ethiopia and evaluate interactions with child health (environmental enteric dysfunction and stunting) in the context of their sociodemographic environment. METHODS AND ANALYSIS: This longitudinal study involves 115 infants who are followed from birth to 12 months of age and are selected randomly from 10 kebeles of Haramaya woreda, East Hararghe zone, Oromia region, Ethiopia. Questionnaire-based information is obtained on demographics, livelihoods, wealth, health, nutrition and women empowerment; animal ownership/management and diseases; and water, sanitation and hygiene. Faecal samples are collected from infants, mothers, siblings and livestock, drinking water and soil. These samples are analysed by a range of phenotypic and genotypic microbiological methods to characterise the genetic structure of the Campylobacter population in each of these reservoirs, which will support inference about the main sources of exposure for infants. ETHICS AND DISSEMINATION: Ethical approval was obtained from the University of Florida Internal Review Board (IRB201903141), the Haramaya University Institutional Health Research Ethics Committee (COHMS/1010/3796/20) and the Ethiopia National Research Ethics Review Committee (SM/14.1/1059/20). Written informed consent is obtained from all participating households. Research findings will be disseminated to stakeholders through conferences and peer-reviewed journals and through the Feed the Future Innovation Lab for Livestock Systems. BMJ Publishing Group 2022-10-05 /pmc/articles/PMC9535169/ /pubmed/36198455 http://dx.doi.org/10.1136/bmjopen-2022-061311 Text en © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY. Published by BMJ. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Global Health Havelaar, Arie H Brhane, Mussie Ahmed, Ibsa Abdusemed Kedir, Jafer Chen, Dehao Deblais, Loic French, Nigel Gebreyes, Wondwossen A Hassen, Jemal Yousuf Li, Xiaolong Manary, Mark J Mekuria, Zelealem Ibrahim, Abdulmuen Mohammed Mummed, Bahar Ojeda, Amanda Rajashekara, Gireesh Roba, Kedir Teji Saleem, Cyrus Singh, Nitya Usmane, Ibsa Aliyi Yang, Yang Yimer, Getnet McKune, Sarah Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions |
title | Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions |
title_full | Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions |
title_fullStr | Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions |
title_full_unstemmed | Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions |
title_short | Unravelling the reservoirs for colonisation of infants with Campylobacter spp. in rural Ethiopia: protocol for a longitudinal study during a global pandemic and political tensions |
title_sort | unravelling the reservoirs for colonisation of infants with campylobacter spp. in rural ethiopia: protocol for a longitudinal study during a global pandemic and political tensions |
topic | Global Health |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535169/ https://www.ncbi.nlm.nih.gov/pubmed/36198455 http://dx.doi.org/10.1136/bmjopen-2022-061311 |
work_keys_str_mv | AT havelaararieh unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT brhanemussie unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT ahmedibsaabdusemed unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT kedirjafer unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT chendehao unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT deblaisloic unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT frenchnigel unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT gebreyeswondwossena unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT hassenjemalyousuf unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT lixiaolong unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT manarymarkj unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT mekuriazelealem unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT ibrahimabdulmuenmohammed unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT mummedbahar unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT ojedaamanda unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT rajashekaragireesh unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT robakedirteji unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT saleemcyrus unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT singhnitya unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT usmaneibsaaliyi unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT yangyang unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT yimergetnet unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions AT mckunesarah unravellingthereservoirsforcolonisationofinfantswithcampylobactersppinruralethiopiaprotocolforalongitudinalstudyduringaglobalpandemicandpoliticaltensions |