Cargando…
Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice
We used mouse CRC cell line (MC38) to establish a heterotopic mouse model, and applied [(89)Zr]-labeled PD-L1 antibody KN035 for PET imaging. Attenuated Salmonella typhimurium 3261 was used as an anti-tumor vaccine, and the combined anti-tumor immunotherapy with bivalent genetic vaccine and anti-PD1...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535276/ https://www.ncbi.nlm.nih.gov/pubmed/36212004 http://dx.doi.org/10.1016/j.heliyon.2022.e10775 |
_version_ | 1784802735604891648 |
---|---|
author | Li, Yuzhen Zhu, Xiaodan You, Jianliang Zhang, Baonan Huang, Xiaona Jin, Chunhui |
author_facet | Li, Yuzhen Zhu, Xiaodan You, Jianliang Zhang, Baonan Huang, Xiaona Jin, Chunhui |
author_sort | Li, Yuzhen |
collection | PubMed |
description | We used mouse CRC cell line (MC38) to establish a heterotopic mouse model, and applied [(89)Zr]-labeled PD-L1 antibody KN035 for PET imaging. Attenuated Salmonella typhimurium 3261 was used as an anti-tumor vaccine, and the combined anti-tumor immunotherapy with bivalent genetic vaccine and anti-PD1 antibody Nivolumab was conducted. MicroPET was performed to observe the changes of tumor tissues and expression of PD-L1. We found that the recombinant double-gene plasmids were stably expressed in COS7 cells. Study results showed the combined immunotherapy improved the effectiveness over genetic vaccine alone. This study supports that combination of genetic vaccines and anti-immunocheckpoint immunotherapy can inhibit MC38 tumor growth. |
format | Online Article Text |
id | pubmed-9535276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-95352762022-10-07 Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice Li, Yuzhen Zhu, Xiaodan You, Jianliang Zhang, Baonan Huang, Xiaona Jin, Chunhui Heliyon Research Article We used mouse CRC cell line (MC38) to establish a heterotopic mouse model, and applied [(89)Zr]-labeled PD-L1 antibody KN035 for PET imaging. Attenuated Salmonella typhimurium 3261 was used as an anti-tumor vaccine, and the combined anti-tumor immunotherapy with bivalent genetic vaccine and anti-PD1 antibody Nivolumab was conducted. MicroPET was performed to observe the changes of tumor tissues and expression of PD-L1. We found that the recombinant double-gene plasmids were stably expressed in COS7 cells. Study results showed the combined immunotherapy improved the effectiveness over genetic vaccine alone. This study supports that combination of genetic vaccines and anti-immunocheckpoint immunotherapy can inhibit MC38 tumor growth. Elsevier 2022-09-28 /pmc/articles/PMC9535276/ /pubmed/36212004 http://dx.doi.org/10.1016/j.heliyon.2022.e10775 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Li, Yuzhen Zhu, Xiaodan You, Jianliang Zhang, Baonan Huang, Xiaona Jin, Chunhui Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice |
title | Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice |
title_full | Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice |
title_fullStr | Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice |
title_full_unstemmed | Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice |
title_short | Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice |
title_sort | efficacy of bivalent ceacam6/4-1bbl genetic vaccine combined with anti-pd1 antibody in mc38 tumor model of mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535276/ https://www.ncbi.nlm.nih.gov/pubmed/36212004 http://dx.doi.org/10.1016/j.heliyon.2022.e10775 |
work_keys_str_mv | AT liyuzhen efficacyofbivalentceacam641bblgeneticvaccinecombinedwithantipd1antibodyinmc38tumormodelofmice AT zhuxiaodan efficacyofbivalentceacam641bblgeneticvaccinecombinedwithantipd1antibodyinmc38tumormodelofmice AT youjianliang efficacyofbivalentceacam641bblgeneticvaccinecombinedwithantipd1antibodyinmc38tumormodelofmice AT zhangbaonan efficacyofbivalentceacam641bblgeneticvaccinecombinedwithantipd1antibodyinmc38tumormodelofmice AT huangxiaona efficacyofbivalentceacam641bblgeneticvaccinecombinedwithantipd1antibodyinmc38tumormodelofmice AT jinchunhui efficacyofbivalentceacam641bblgeneticvaccinecombinedwithantipd1antibodyinmc38tumormodelofmice |