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Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice

We used mouse CRC cell line (MC38) to establish a heterotopic mouse model, and applied [(89)Zr]-labeled PD-L1 antibody KN035 for PET imaging. Attenuated Salmonella typhimurium 3261 was used as an anti-tumor vaccine, and the combined anti-tumor immunotherapy with bivalent genetic vaccine and anti-PD1...

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Detalles Bibliográficos
Autores principales: Li, Yuzhen, Zhu, Xiaodan, You, Jianliang, Zhang, Baonan, Huang, Xiaona, Jin, Chunhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535276/
https://www.ncbi.nlm.nih.gov/pubmed/36212004
http://dx.doi.org/10.1016/j.heliyon.2022.e10775
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author Li, Yuzhen
Zhu, Xiaodan
You, Jianliang
Zhang, Baonan
Huang, Xiaona
Jin, Chunhui
author_facet Li, Yuzhen
Zhu, Xiaodan
You, Jianliang
Zhang, Baonan
Huang, Xiaona
Jin, Chunhui
author_sort Li, Yuzhen
collection PubMed
description We used mouse CRC cell line (MC38) to establish a heterotopic mouse model, and applied [(89)Zr]-labeled PD-L1 antibody KN035 for PET imaging. Attenuated Salmonella typhimurium 3261 was used as an anti-tumor vaccine, and the combined anti-tumor immunotherapy with bivalent genetic vaccine and anti-PD1 antibody Nivolumab was conducted. MicroPET was performed to observe the changes of tumor tissues and expression of PD-L1. We found that the recombinant double-gene plasmids were stably expressed in COS7 cells. Study results showed the combined immunotherapy improved the effectiveness over genetic vaccine alone. This study supports that combination of genetic vaccines and anti-immunocheckpoint immunotherapy can inhibit MC38 tumor growth.
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spelling pubmed-95352762022-10-07 Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice Li, Yuzhen Zhu, Xiaodan You, Jianliang Zhang, Baonan Huang, Xiaona Jin, Chunhui Heliyon Research Article We used mouse CRC cell line (MC38) to establish a heterotopic mouse model, and applied [(89)Zr]-labeled PD-L1 antibody KN035 for PET imaging. Attenuated Salmonella typhimurium 3261 was used as an anti-tumor vaccine, and the combined anti-tumor immunotherapy with bivalent genetic vaccine and anti-PD1 antibody Nivolumab was conducted. MicroPET was performed to observe the changes of tumor tissues and expression of PD-L1. We found that the recombinant double-gene plasmids were stably expressed in COS7 cells. Study results showed the combined immunotherapy improved the effectiveness over genetic vaccine alone. This study supports that combination of genetic vaccines and anti-immunocheckpoint immunotherapy can inhibit MC38 tumor growth. Elsevier 2022-09-28 /pmc/articles/PMC9535276/ /pubmed/36212004 http://dx.doi.org/10.1016/j.heliyon.2022.e10775 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Li, Yuzhen
Zhu, Xiaodan
You, Jianliang
Zhang, Baonan
Huang, Xiaona
Jin, Chunhui
Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice
title Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice
title_full Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice
title_fullStr Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice
title_full_unstemmed Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice
title_short Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice
title_sort efficacy of bivalent ceacam6/4-1bbl genetic vaccine combined with anti-pd1 antibody in mc38 tumor model of mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535276/
https://www.ncbi.nlm.nih.gov/pubmed/36212004
http://dx.doi.org/10.1016/j.heliyon.2022.e10775
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