Near-infrared nuclear markers for Drosophila imaging

Nuclear markers for live imaging are useful for counting and tracking cells, visualizing cell division, and examining the regulation of proteins that are controlled via entry or exit from the nucleus. Near-infrared fluorescent proteins have advantages over shorter wavelength fluorescent proteins, in...

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Detalles Bibliográficos
Autores principales: Pan, Yuanwang, Rauskolb, Cordelia, Irvine, Kenneth D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535449/
https://www.ncbi.nlm.nih.gov/pubmed/36212517
http://dx.doi.org/10.17912/micropub.biology.000639
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author Pan, Yuanwang
Rauskolb, Cordelia
Irvine, Kenneth D
author_facet Pan, Yuanwang
Rauskolb, Cordelia
Irvine, Kenneth D
author_sort Pan, Yuanwang
collection PubMed
description Nuclear markers for live imaging are useful for counting and tracking cells, visualizing cell division, and examining the regulation of proteins that are controlled via entry or exit from the nucleus. Near-infrared fluorescent proteins have advantages over shorter wavelength fluorescent proteins, including reduced phototoxicity, less light scattering, and enabling multicolor live imaging. We have constructed and tested transgenic Drosophila expressing Histone H2Av iRFP fusion proteins, and confirmed that they can be used to label nuclei in both fixed and live tissue at multiple stages of development.
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spelling pubmed-95354492022-10-07 Near-infrared nuclear markers for Drosophila imaging Pan, Yuanwang Rauskolb, Cordelia Irvine, Kenneth D MicroPubl Biol Materials and Reagents Nuclear markers for live imaging are useful for counting and tracking cells, visualizing cell division, and examining the regulation of proteins that are controlled via entry or exit from the nucleus. Near-infrared fluorescent proteins have advantages over shorter wavelength fluorescent proteins, including reduced phototoxicity, less light scattering, and enabling multicolor live imaging. We have constructed and tested transgenic Drosophila expressing Histone H2Av iRFP fusion proteins, and confirmed that they can be used to label nuclei in both fixed and live tissue at multiple stages of development. Caltech Library 2022-09-21 /pmc/articles/PMC9535449/ /pubmed/36212517 http://dx.doi.org/10.17912/micropub.biology.000639 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Pan, Yuanwang
Rauskolb, Cordelia
Irvine, Kenneth D
Near-infrared nuclear markers for Drosophila imaging
title Near-infrared nuclear markers for Drosophila imaging
title_full Near-infrared nuclear markers for Drosophila imaging
title_fullStr Near-infrared nuclear markers for Drosophila imaging
title_full_unstemmed Near-infrared nuclear markers for Drosophila imaging
title_short Near-infrared nuclear markers for Drosophila imaging
title_sort near-infrared nuclear markers for drosophila imaging
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535449/
https://www.ncbi.nlm.nih.gov/pubmed/36212517
http://dx.doi.org/10.17912/micropub.biology.000639
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AT rauskolbcordelia nearinfrarednuclearmarkersfordrosophilaimaging
AT irvinekennethd nearinfrarednuclearmarkersfordrosophilaimaging

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