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Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality
Background: Eggshell strength and thickness are critical factors in reducing the egg breaking rate and preventing economic losses. The calcite biomineralization process is very important for eggshell quality. Therefore, we employed transcriptional sequencing and proteomics to investigate the differe...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536113/ https://www.ncbi.nlm.nih.gov/pubmed/36212119 http://dx.doi.org/10.3389/fgene.2022.927670 |
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author | Wu, Yan Sun, Yanyan Zhang, Hao Xiao, Hongwei Pan, Ailuan Shen, Jie Pu, Yuejin Liang, Zhenhua Du, Jinping Pi, Jinsong |
author_facet | Wu, Yan Sun, Yanyan Zhang, Hao Xiao, Hongwei Pan, Ailuan Shen, Jie Pu, Yuejin Liang, Zhenhua Du, Jinping Pi, Jinsong |
author_sort | Wu, Yan |
collection | PubMed |
description | Background: Eggshell strength and thickness are critical factors in reducing the egg breaking rate and preventing economic losses. The calcite biomineralization process is very important for eggshell quality. Therefore, we employed transcriptional sequencing and proteomics to investigate the differences between the uteruses of laying hens with high- and low-breaking-strength shells. Results: A total of 1,028 differentially expressed genes (DEGs) and 270 differentially expressed proteins (DEPs) were identified. The analysis results of GO terms and KEGG pathways showed that most of the DEGs and DEPs were enriched in vital pathways related to processes such as calcium metabolism, hormone and amino acid biosynthesis, and cell proliferation and apoptosis. Several DEGs and DEPs that were coexpressed at mRNA and protein levels were verified. KRT14 (keratin-14) is a candidate gene (protein) obtained by multiple omics analysis due to the fold difference of KRT14 being the largest. After the overexpression of KRT14 in uterine epithelial cells, the expressions of OC116 (ovocleididin-116), CALB1 (calbindin 1), and BST1 (ADP-ribosyl cyclase 2) were found to be increased significantly, while the expression of OC17 (ovocleididin-17) was found to be decreased significantly. Conclusion: In summary, this study confirms that during normal calcification, there are differences in ion transport between the uterus of hens producing high-breaking-strength eggshells and those producing low-breaking-strength eggshells, which may help elucidate the eggshell calcification process. The KRT14 gene may promote calcium metabolism and deposition of calcium carbonate in eggshells. |
format | Online Article Text |
id | pubmed-9536113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95361132022-10-07 Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality Wu, Yan Sun, Yanyan Zhang, Hao Xiao, Hongwei Pan, Ailuan Shen, Jie Pu, Yuejin Liang, Zhenhua Du, Jinping Pi, Jinsong Front Genet Genetics Background: Eggshell strength and thickness are critical factors in reducing the egg breaking rate and preventing economic losses. The calcite biomineralization process is very important for eggshell quality. Therefore, we employed transcriptional sequencing and proteomics to investigate the differences between the uteruses of laying hens with high- and low-breaking-strength shells. Results: A total of 1,028 differentially expressed genes (DEGs) and 270 differentially expressed proteins (DEPs) were identified. The analysis results of GO terms and KEGG pathways showed that most of the DEGs and DEPs were enriched in vital pathways related to processes such as calcium metabolism, hormone and amino acid biosynthesis, and cell proliferation and apoptosis. Several DEGs and DEPs that were coexpressed at mRNA and protein levels were verified. KRT14 (keratin-14) is a candidate gene (protein) obtained by multiple omics analysis due to the fold difference of KRT14 being the largest. After the overexpression of KRT14 in uterine epithelial cells, the expressions of OC116 (ovocleididin-116), CALB1 (calbindin 1), and BST1 (ADP-ribosyl cyclase 2) were found to be increased significantly, while the expression of OC17 (ovocleididin-17) was found to be decreased significantly. Conclusion: In summary, this study confirms that during normal calcification, there are differences in ion transport between the uterus of hens producing high-breaking-strength eggshells and those producing low-breaking-strength eggshells, which may help elucidate the eggshell calcification process. The KRT14 gene may promote calcium metabolism and deposition of calcium carbonate in eggshells. Frontiers Media S.A. 2022-09-22 /pmc/articles/PMC9536113/ /pubmed/36212119 http://dx.doi.org/10.3389/fgene.2022.927670 Text en Copyright © 2022 Wu, Sun, Zhang, Xiao, Pan, Shen, Pu, Liang, Du and Pi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Wu, Yan Sun, Yanyan Zhang, Hao Xiao, Hongwei Pan, Ailuan Shen, Jie Pu, Yuejin Liang, Zhenhua Du, Jinping Pi, Jinsong Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality |
title | Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality |
title_full | Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality |
title_fullStr | Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality |
title_full_unstemmed | Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality |
title_short | Multiomic analysis revealed the regulatory role of the KRT14 gene in eggshell quality |
title_sort | multiomic analysis revealed the regulatory role of the krt14 gene in eggshell quality |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536113/ https://www.ncbi.nlm.nih.gov/pubmed/36212119 http://dx.doi.org/10.3389/fgene.2022.927670 |
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