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Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia

The carotid body (CB) contributes significantly to oxygen sensing. It is unclear, however, whether glutamatergic signaling is involved in the CB response to hypoxia. Previously, we reported that ionotropic glutamate receptors (iGluRs) and multiple glutamate transporters are present in the rat CB. Ex...

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Autores principales: Zhao, Chenlu, Li, Chaohong, Zhao, Baosheng, Liu, Yuzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536148/
https://www.ncbi.nlm.nih.gov/pubmed/36213225
http://dx.doi.org/10.3389/fphys.2022.1008073
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author Zhao, Chenlu
Li, Chaohong
Zhao, Baosheng
Liu, Yuzhen
author_facet Zhao, Chenlu
Li, Chaohong
Zhao, Baosheng
Liu, Yuzhen
author_sort Zhao, Chenlu
collection PubMed
description The carotid body (CB) contributes significantly to oxygen sensing. It is unclear, however, whether glutamatergic signaling is involved in the CB response to hypoxia. Previously, we reported that ionotropic glutamate receptors (iGluRs) and multiple glutamate transporters are present in the rat CB. Except for iGluRs, glutamate receptors also include metabotropic glutamate receptors (mGluRs), which are divided into the following groups: Group I (mGluR1/5); group II (mGluR2/3); group III (mGluR4/6/7/8). We have studied the expression of group I mGluRs in the rat CB and its physiological function response to acute hypoxia. To further elucidate the states of mGluRs in the CB, this study’s aim was to investigate the expression of group II and III mGluRs and the response of rat CB to acute hypoxia. We used reverse transcription-polymerase chain reaction (RT-PCR) to observed mRNA expression of GRM2/3/4/6/7/8 subunits by using immunostaining to show the distribution of mGluR2 and mGluR8. The results revealed that the GRM2/3/4/6/7/8 mRNAs were expressed in both rat and human CB. Immunostaining showed that mGluR2 was localized in the type I cells and mGluR8 was localized in type I and type II cells in the rat CB. Moreover, the response of CB to acute hypoxia in rats was recorded by in vitro carotid sinus nerve (CSN) discharge. Perfusion of group II mGluRs agonist or group III mGluRs agonist (LY379268 or L-SOP) was applied to examine the effect of group II and III mGluRs on rat CB response to acute hypoxia. We found that LY379268 and L-SOP inhibited hypoxia-induced enhancement of CSN activity. Based on the above findings, group II and III mGluRs appear to play an inhibitory role in the carotid chemoreceptor response to acute hypoxia.
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spelling pubmed-95361482022-10-07 Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia Zhao, Chenlu Li, Chaohong Zhao, Baosheng Liu, Yuzhen Front Physiol Physiology The carotid body (CB) contributes significantly to oxygen sensing. It is unclear, however, whether glutamatergic signaling is involved in the CB response to hypoxia. Previously, we reported that ionotropic glutamate receptors (iGluRs) and multiple glutamate transporters are present in the rat CB. Except for iGluRs, glutamate receptors also include metabotropic glutamate receptors (mGluRs), which are divided into the following groups: Group I (mGluR1/5); group II (mGluR2/3); group III (mGluR4/6/7/8). We have studied the expression of group I mGluRs in the rat CB and its physiological function response to acute hypoxia. To further elucidate the states of mGluRs in the CB, this study’s aim was to investigate the expression of group II and III mGluRs and the response of rat CB to acute hypoxia. We used reverse transcription-polymerase chain reaction (RT-PCR) to observed mRNA expression of GRM2/3/4/6/7/8 subunits by using immunostaining to show the distribution of mGluR2 and mGluR8. The results revealed that the GRM2/3/4/6/7/8 mRNAs were expressed in both rat and human CB. Immunostaining showed that mGluR2 was localized in the type I cells and mGluR8 was localized in type I and type II cells in the rat CB. Moreover, the response of CB to acute hypoxia in rats was recorded by in vitro carotid sinus nerve (CSN) discharge. Perfusion of group II mGluRs agonist or group III mGluRs agonist (LY379268 or L-SOP) was applied to examine the effect of group II and III mGluRs on rat CB response to acute hypoxia. We found that LY379268 and L-SOP inhibited hypoxia-induced enhancement of CSN activity. Based on the above findings, group II and III mGluRs appear to play an inhibitory role in the carotid chemoreceptor response to acute hypoxia. Frontiers Media S.A. 2022-09-22 /pmc/articles/PMC9536148/ /pubmed/36213225 http://dx.doi.org/10.3389/fphys.2022.1008073 Text en Copyright © 2022 Zhao, Li, Zhao and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Zhao, Chenlu
Li, Chaohong
Zhao, Baosheng
Liu, Yuzhen
Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia
title Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia
title_full Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia
title_fullStr Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia
title_full_unstemmed Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia
title_short Expression of group II and III mGluRs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia
title_sort expression of group ii and iii mglurs in the carotid body and its role in the carotid chemoreceptor response to acute hypoxia
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536148/
https://www.ncbi.nlm.nih.gov/pubmed/36213225
http://dx.doi.org/10.3389/fphys.2022.1008073
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