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The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury

Acute skeletal muscle injury is followed by satellite cell activation, proliferation, and differentiation to replace damaged fibers with newly regenerated muscle fibers, processes that involve satellite cell interactions with various niche signals. Here we show that satellite cell specific deletion...

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Autores principales: Shams, Ahmed S., Arpke, Robert W., Gearhart, Micah D., Weiblen, Johannes, Mai, Ben, Oyler, David, Bosnakovski, Darko, Mahmoud, Omayma M., Hassan, Gamal M., Kyba, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536311/
https://www.ncbi.nlm.nih.gov/pubmed/36211464
http://dx.doi.org/10.3389/fcell.2022.949532
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author Shams, Ahmed S.
Arpke, Robert W.
Gearhart, Micah D.
Weiblen, Johannes
Mai, Ben
Oyler, David
Bosnakovski, Darko
Mahmoud, Omayma M.
Hassan, Gamal M.
Kyba, Michael
author_facet Shams, Ahmed S.
Arpke, Robert W.
Gearhart, Micah D.
Weiblen, Johannes
Mai, Ben
Oyler, David
Bosnakovski, Darko
Mahmoud, Omayma M.
Hassan, Gamal M.
Kyba, Michael
author_sort Shams, Ahmed S.
collection PubMed
description Acute skeletal muscle injury is followed by satellite cell activation, proliferation, and differentiation to replace damaged fibers with newly regenerated muscle fibers, processes that involve satellite cell interactions with various niche signals. Here we show that satellite cell specific deletion of the chemokine receptor CXCR4, followed by suppression of recombination escapers, leads to defects in regeneration and satellite cell pool repopulation in both the transplantation and in situ injury contexts. Mechanistically, we show that endothelial cells and FAPs express the gene for the ligand, SDF1α, and that CXCR4 is principally required for proper activation and for transit through the first cell division, and to a lesser extent the later cell divisions. In the absence of CXCR4, gene expression in quiescent satellite cells is not severely disrupted, but in activated satellite cells a subset of genes normally induced by activation fail to upregulate normally. These data demonstrate that CXCR4 signaling is essential to normal early activation, proliferation, and self-renewal of satellite cells.
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spelling pubmed-95363112022-10-07 The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury Shams, Ahmed S. Arpke, Robert W. Gearhart, Micah D. Weiblen, Johannes Mai, Ben Oyler, David Bosnakovski, Darko Mahmoud, Omayma M. Hassan, Gamal M. Kyba, Michael Front Cell Dev Biol Cell and Developmental Biology Acute skeletal muscle injury is followed by satellite cell activation, proliferation, and differentiation to replace damaged fibers with newly regenerated muscle fibers, processes that involve satellite cell interactions with various niche signals. Here we show that satellite cell specific deletion of the chemokine receptor CXCR4, followed by suppression of recombination escapers, leads to defects in regeneration and satellite cell pool repopulation in both the transplantation and in situ injury contexts. Mechanistically, we show that endothelial cells and FAPs express the gene for the ligand, SDF1α, and that CXCR4 is principally required for proper activation and for transit through the first cell division, and to a lesser extent the later cell divisions. In the absence of CXCR4, gene expression in quiescent satellite cells is not severely disrupted, but in activated satellite cells a subset of genes normally induced by activation fail to upregulate normally. These data demonstrate that CXCR4 signaling is essential to normal early activation, proliferation, and self-renewal of satellite cells. Frontiers Media S.A. 2022-09-22 /pmc/articles/PMC9536311/ /pubmed/36211464 http://dx.doi.org/10.3389/fcell.2022.949532 Text en Copyright © 2022 Shams, Arpke, Gearhart, Weiblen, Mai, Oyler, Bosnakovski, Mahmoud, Hassan and Kyba. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Shams, Ahmed S.
Arpke, Robert W.
Gearhart, Micah D.
Weiblen, Johannes
Mai, Ben
Oyler, David
Bosnakovski, Darko
Mahmoud, Omayma M.
Hassan, Gamal M.
Kyba, Michael
The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury
title The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury
title_full The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury
title_fullStr The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury
title_full_unstemmed The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury
title_short The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury
title_sort chemokine receptor cxcr4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536311/
https://www.ncbi.nlm.nih.gov/pubmed/36211464
http://dx.doi.org/10.3389/fcell.2022.949532
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