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The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma
To further determine how BHE affected the growth of HCC cells, the proportion of each cell cycle phase was explored in HCC cells by flow cytometry. Blue honeysuckle (Lonicera caerulea L.) is a species of bush that grows in eastern Russia. Blue honeysuckle extract (BHE) is rich in bioactive phytochem...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536902/ https://www.ncbi.nlm.nih.gov/pubmed/36212967 http://dx.doi.org/10.1155/2022/9601020 |
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author | Zhang, Chun-Peng Li, Wei-Hua Liu, Jia-Ren Li, Guo-Dong Zhang, Hao-Peng Wei, Jiu-Feng Chen, Hong-Sheng Zhao, Jin-Lu Wang, Yun-Feng Lv, Qiang Liu, Ming |
author_facet | Zhang, Chun-Peng Li, Wei-Hua Liu, Jia-Ren Li, Guo-Dong Zhang, Hao-Peng Wei, Jiu-Feng Chen, Hong-Sheng Zhao, Jin-Lu Wang, Yun-Feng Lv, Qiang Liu, Ming |
author_sort | Zhang, Chun-Peng |
collection | PubMed |
description | To further determine how BHE affected the growth of HCC cells, the proportion of each cell cycle phase was explored in HCC cells by flow cytometry. Blue honeysuckle (Lonicera caerulea L.) is a species of bush that grows in eastern Russia. Blue honeysuckle extract (BHE) is rich in bioactive phytochemicals which can inhibit the proliferation of tumor cells. The mechanism underlying the anticancer activity of BHE in primary liver cancer is poorly understood. The purpose of this study was to evaluate the growth inhibition mechanism of bioactive substances from blue honeysuckle on hepatocellular carcinoma (HCC) cells and to explore its protein and gene targets. The compounds in BHE were determined by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Cell counting kit-8 (CCK8) assay was used to evaluate the effects of BHE on HCC cell proliferation, and flow cytometry assay (FCA) was used to determine how BHE arrested the proportion of each cell cycle phase in HCC cells. Western blot (WB) was performed to determine the expression of cell cycle-related proteins in HCC cells treated with different concentrations of BHE. The xenograft tumor animal models were established by HCC cell implantation. The results showed that cyanidin-3-o-glucoside and cyanidin-3-o-sophoroside which are the main biologically active components were detected in BHE. BHE is highly effective in inhibiting the proliferation of HCC cells by arresting the HCC cell cycle in the G2/M phase. BHE also downregulated the expression of conventional or classical dendritic cells-2 (cDC2) and cyclin B1 by promoting the expression of myelin transcription factor 1 (MyT1) in HCC cells. The weight and volume of xenografts were significantly decreased in the BHE treated groups when compared to the control group. BHE increased the expression of MyT1 in xenograft tissues. These findings showed that blue honeysuckle extract inhibits proliferation in vivo and in vitro by downregulating the expression of cDC2 and cyclin B1 and upregulating the expression of MyT1 in HCC cells. |
format | Online Article Text |
id | pubmed-9536902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95369022022-10-07 The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma Zhang, Chun-Peng Li, Wei-Hua Liu, Jia-Ren Li, Guo-Dong Zhang, Hao-Peng Wei, Jiu-Feng Chen, Hong-Sheng Zhao, Jin-Lu Wang, Yun-Feng Lv, Qiang Liu, Ming Evid Based Complement Alternat Med Research Article To further determine how BHE affected the growth of HCC cells, the proportion of each cell cycle phase was explored in HCC cells by flow cytometry. Blue honeysuckle (Lonicera caerulea L.) is a species of bush that grows in eastern Russia. Blue honeysuckle extract (BHE) is rich in bioactive phytochemicals which can inhibit the proliferation of tumor cells. The mechanism underlying the anticancer activity of BHE in primary liver cancer is poorly understood. The purpose of this study was to evaluate the growth inhibition mechanism of bioactive substances from blue honeysuckle on hepatocellular carcinoma (HCC) cells and to explore its protein and gene targets. The compounds in BHE were determined by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Cell counting kit-8 (CCK8) assay was used to evaluate the effects of BHE on HCC cell proliferation, and flow cytometry assay (FCA) was used to determine how BHE arrested the proportion of each cell cycle phase in HCC cells. Western blot (WB) was performed to determine the expression of cell cycle-related proteins in HCC cells treated with different concentrations of BHE. The xenograft tumor animal models were established by HCC cell implantation. The results showed that cyanidin-3-o-glucoside and cyanidin-3-o-sophoroside which are the main biologically active components were detected in BHE. BHE is highly effective in inhibiting the proliferation of HCC cells by arresting the HCC cell cycle in the G2/M phase. BHE also downregulated the expression of conventional or classical dendritic cells-2 (cDC2) and cyclin B1 by promoting the expression of myelin transcription factor 1 (MyT1) in HCC cells. The weight and volume of xenografts were significantly decreased in the BHE treated groups when compared to the control group. BHE increased the expression of MyT1 in xenograft tissues. These findings showed that blue honeysuckle extract inhibits proliferation in vivo and in vitro by downregulating the expression of cDC2 and cyclin B1 and upregulating the expression of MyT1 in HCC cells. Hindawi 2022-09-29 /pmc/articles/PMC9536902/ /pubmed/36212967 http://dx.doi.org/10.1155/2022/9601020 Text en Copyright © 2022 Chun-Peng Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Chun-Peng Li, Wei-Hua Liu, Jia-Ren Li, Guo-Dong Zhang, Hao-Peng Wei, Jiu-Feng Chen, Hong-Sheng Zhao, Jin-Lu Wang, Yun-Feng Lv, Qiang Liu, Ming The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma |
title | The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma |
title_full | The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma |
title_fullStr | The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma |
title_full_unstemmed | The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma |
title_short | The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma |
title_sort | effective analysis for blue honeysuckle extract in the treatment of hepatocellular carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536902/ https://www.ncbi.nlm.nih.gov/pubmed/36212967 http://dx.doi.org/10.1155/2022/9601020 |
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