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Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice

PURPOSE: To assess functional and structural changes in vascular and neural structures associated with diabetic bladder dysfunction (DBD) in the bladders of streptozotocin (STZ)-induced diabetic mice. METHODS: Eight-week-old C57BL/6 mice were injected with STZ at 50 mg/kg daily for 5 consecutive day...

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Autores principales: Kwon, Mi-Hye, Choi, Min-Ji, Liu, Fang-Yuan, Fridayana, Fitri Rahma, Niloofar, Lashkari, Yin, Guo Nan, Ryu, Ji-Kan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Continence Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9537429/
https://www.ncbi.nlm.nih.gov/pubmed/36203252
http://dx.doi.org/10.5213/inj.2244152.076
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author Kwon, Mi-Hye
Choi, Min-Ji
Liu, Fang-Yuan
Fridayana, Fitri Rahma
Niloofar, Lashkari
Yin, Guo Nan
Ryu, Ji-Kan
author_facet Kwon, Mi-Hye
Choi, Min-Ji
Liu, Fang-Yuan
Fridayana, Fitri Rahma
Niloofar, Lashkari
Yin, Guo Nan
Ryu, Ji-Kan
author_sort Kwon, Mi-Hye
collection PubMed
description PURPOSE: To assess functional and structural changes in vascular and neural structures associated with diabetic bladder dysfunction (DBD) in the bladders of streptozotocin (STZ)-induced diabetic mice. METHODS: Eight-week-old C57BL/6 mice were injected with STZ at 50 mg/kg daily for 5 consecutive days. Catheters were inserted 12 weeks later, and 5 days after catheter placement bladder functions were assessed by conscious cystometry. Neurovascular and extracellular matrix marker changes in harvested urinary bladders were investigated by immunofluorescent staining. Body weights and fasting and postprandial blood glucose levels were measured 12 weeks after STZ injection. RESULTS: STZ-induced diabetic mice had significantly lower body weights and significantly higher blood glucose levels. Assessment of bladder function in STZ-induced diabetic mice revealed a nearly 3-fold increase in bladder capacity and intercontractile interval compared to controls. However, basal pressure, maximal bladder pressure, and threshold pressure were not significantly different. Morphological and structural analysis showed that STZ-induced diabetic mice had significantly reduced microvascular density in lamina propria (33% of the nondiabetic control values), and severely decreased nerve contents in the detrusor region (42% of the nondiabetic control values). CONCLUSIONS: STZ-induced diabetic mice exhibit functional and structural derangements in urinary bladder. The present study provides a foundation and describes a useful means of evaluating the efficacies of therapeutic targets and exploring the detailed mechanism of DBD.
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spelling pubmed-95374292022-10-14 Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice Kwon, Mi-Hye Choi, Min-Ji Liu, Fang-Yuan Fridayana, Fitri Rahma Niloofar, Lashkari Yin, Guo Nan Ryu, Ji-Kan Int Neurourol J Original Article PURPOSE: To assess functional and structural changes in vascular and neural structures associated with diabetic bladder dysfunction (DBD) in the bladders of streptozotocin (STZ)-induced diabetic mice. METHODS: Eight-week-old C57BL/6 mice were injected with STZ at 50 mg/kg daily for 5 consecutive days. Catheters were inserted 12 weeks later, and 5 days after catheter placement bladder functions were assessed by conscious cystometry. Neurovascular and extracellular matrix marker changes in harvested urinary bladders were investigated by immunofluorescent staining. Body weights and fasting and postprandial blood glucose levels were measured 12 weeks after STZ injection. RESULTS: STZ-induced diabetic mice had significantly lower body weights and significantly higher blood glucose levels. Assessment of bladder function in STZ-induced diabetic mice revealed a nearly 3-fold increase in bladder capacity and intercontractile interval compared to controls. However, basal pressure, maximal bladder pressure, and threshold pressure were not significantly different. Morphological and structural analysis showed that STZ-induced diabetic mice had significantly reduced microvascular density in lamina propria (33% of the nondiabetic control values), and severely decreased nerve contents in the detrusor region (42% of the nondiabetic control values). CONCLUSIONS: STZ-induced diabetic mice exhibit functional and structural derangements in urinary bladder. The present study provides a foundation and describes a useful means of evaluating the efficacies of therapeutic targets and exploring the detailed mechanism of DBD. Korean Continence Society 2022-09 2022-09-30 /pmc/articles/PMC9537429/ /pubmed/36203252 http://dx.doi.org/10.5213/inj.2244152.076 Text en Copyright © 2022 Korean Continence Society https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kwon, Mi-Hye
Choi, Min-Ji
Liu, Fang-Yuan
Fridayana, Fitri Rahma
Niloofar, Lashkari
Yin, Guo Nan
Ryu, Ji-Kan
Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice
title Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice
title_full Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice
title_fullStr Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice
title_full_unstemmed Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice
title_short Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice
title_sort functional and immunofluorescence evaluations of vascular and neural integrities in urinary bladder of streptozotocin-induced diabetic mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9537429/
https://www.ncbi.nlm.nih.gov/pubmed/36203252
http://dx.doi.org/10.5213/inj.2244152.076
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