A miniature fluorescence microscope for multi-plane imaging

Miniature fluorescence microscopes are becoming an increasingly established tool to investigate neural circuits in freely moving animals. In this work we present a lightweight one-photon microscope capable of imaging at different focal depths. The focal plane can be changed dynamically by modulating...

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Autores principales: Barbera, Giovanni, Jun, Rachel, Zhang, Yan, Liang, Bo, Li, Yun, Lin, Da-Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9537509/
https://www.ncbi.nlm.nih.gov/pubmed/36202883
http://dx.doi.org/10.1038/s41598-022-21022-9
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author Barbera, Giovanni
Jun, Rachel
Zhang, Yan
Liang, Bo
Li, Yun
Lin, Da-Ting
author_facet Barbera, Giovanni
Jun, Rachel
Zhang, Yan
Liang, Bo
Li, Yun
Lin, Da-Ting
author_sort Barbera, Giovanni
collection PubMed
description Miniature fluorescence microscopes are becoming an increasingly established tool to investigate neural circuits in freely moving animals. In this work we present a lightweight one-photon microscope capable of imaging at different focal depths. The focal plane can be changed dynamically by modulating the pulse width of the control signal to a variable focus liquid lens, which is synchronized to the image sensor to enable changing focal plane between frames. The system was tested by imaging GCaMP7f expressing neurons in the mouse medial prefrontal cortex (mPFC) in vivo during open field test. Results showed that with the proposed design it is possible to image neurons across an axial scan of ~ 60 μm, resulting in a ~ 40% increase of total neurons imaged compared to single plane imaging.
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spelling pubmed-95375092022-10-08 A miniature fluorescence microscope for multi-plane imaging Barbera, Giovanni Jun, Rachel Zhang, Yan Liang, Bo Li, Yun Lin, Da-Ting Sci Rep Article Miniature fluorescence microscopes are becoming an increasingly established tool to investigate neural circuits in freely moving animals. In this work we present a lightweight one-photon microscope capable of imaging at different focal depths. The focal plane can be changed dynamically by modulating the pulse width of the control signal to a variable focus liquid lens, which is synchronized to the image sensor to enable changing focal plane between frames. The system was tested by imaging GCaMP7f expressing neurons in the mouse medial prefrontal cortex (mPFC) in vivo during open field test. Results showed that with the proposed design it is possible to image neurons across an axial scan of ~ 60 μm, resulting in a ~ 40% increase of total neurons imaged compared to single plane imaging. Nature Publishing Group UK 2022-10-06 /pmc/articles/PMC9537509/ /pubmed/36202883 http://dx.doi.org/10.1038/s41598-022-21022-9 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Barbera, Giovanni
Jun, Rachel
Zhang, Yan
Liang, Bo
Li, Yun
Lin, Da-Ting
A miniature fluorescence microscope for multi-plane imaging
title A miniature fluorescence microscope for multi-plane imaging
title_full A miniature fluorescence microscope for multi-plane imaging
title_fullStr A miniature fluorescence microscope for multi-plane imaging
title_full_unstemmed A miniature fluorescence microscope for multi-plane imaging
title_short A miniature fluorescence microscope for multi-plane imaging
title_sort miniature fluorescence microscope for multi-plane imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9537509/
https://www.ncbi.nlm.nih.gov/pubmed/36202883
http://dx.doi.org/10.1038/s41598-022-21022-9
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