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Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis
In order to determine the possible aqueous humor (AH) proteins involved in diabetic nephropathy (DN) progression, we performed gel electrophoresis-liquid chromatography-tandem mass spectrometry protein profiling of AH samples from 5 patients with proliferative diabetic retinopathy (PDR) combined DN...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9537621/ https://www.ncbi.nlm.nih.gov/pubmed/36211816 http://dx.doi.org/10.1155/2022/5945828 |
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author | Chen, Huan Wang, Tan Wang, Erqian Li, Ningning Min, Hanyi |
author_facet | Chen, Huan Wang, Tan Wang, Erqian Li, Ningning Min, Hanyi |
author_sort | Chen, Huan |
collection | PubMed |
description | In order to determine the possible aqueous humor (AH) proteins involved in diabetic nephropathy (DN) progression, we performed gel electrophoresis-liquid chromatography-tandem mass spectrometry protein profiling of AH samples from 5 patients with proliferative diabetic retinopathy (PDR) combined DN and 5 patients with PDR. Function enrichment analyses were carried out after the identification of differentially expressed proteins (DEPs). Protein-protein interaction networks were then built and the Search Tool for the Retrieval of Interacting Genes database and CytoNCA plugin in Cytoscape were utilized for module analysis. Ingenuity Pathway Analysis (IPA) was used to analyze disease and biological function, Tox function enrichment and upstream regulatory molecules/networks. Fifty-four DEPs were finally confirmed, whose enriched functions and pathways covered cell adhesion, extracellular exosome, complement activation, complement and coagulation cascades, etc. Nine hub genes were identified, including NCAM1, PLG, APOH, C3, PSAP, RBP4, CDH2, NUCB1, and GNS. IPA showed that C3 and PLG are involved in renal and urological system abnormalities. Conclusively, DEPs and hub proteins confirmed in this exploratory AH proteomic analysis may help us gain a deeper understanding of the molecular mechanisms involved in DN progression, providing novel candidate biomarkers for the early detection for diagnosis of DN. |
format | Online Article Text |
id | pubmed-9537621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95376212022-10-08 Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis Chen, Huan Wang, Tan Wang, Erqian Li, Ningning Min, Hanyi Oxid Med Cell Longev Research Article In order to determine the possible aqueous humor (AH) proteins involved in diabetic nephropathy (DN) progression, we performed gel electrophoresis-liquid chromatography-tandem mass spectrometry protein profiling of AH samples from 5 patients with proliferative diabetic retinopathy (PDR) combined DN and 5 patients with PDR. Function enrichment analyses were carried out after the identification of differentially expressed proteins (DEPs). Protein-protein interaction networks were then built and the Search Tool for the Retrieval of Interacting Genes database and CytoNCA plugin in Cytoscape were utilized for module analysis. Ingenuity Pathway Analysis (IPA) was used to analyze disease and biological function, Tox function enrichment and upstream regulatory molecules/networks. Fifty-four DEPs were finally confirmed, whose enriched functions and pathways covered cell adhesion, extracellular exosome, complement activation, complement and coagulation cascades, etc. Nine hub genes were identified, including NCAM1, PLG, APOH, C3, PSAP, RBP4, CDH2, NUCB1, and GNS. IPA showed that C3 and PLG are involved in renal and urological system abnormalities. Conclusively, DEPs and hub proteins confirmed in this exploratory AH proteomic analysis may help us gain a deeper understanding of the molecular mechanisms involved in DN progression, providing novel candidate biomarkers for the early detection for diagnosis of DN. Hindawi 2022-09-29 /pmc/articles/PMC9537621/ /pubmed/36211816 http://dx.doi.org/10.1155/2022/5945828 Text en Copyright © 2022 Huan Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Huan Wang, Tan Wang, Erqian Li, Ningning Min, Hanyi Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis |
title | Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis |
title_full | Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis |
title_fullStr | Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis |
title_full_unstemmed | Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis |
title_short | Pursuing Diabetic Nephropathy through Aqueous Humor Proteomics Analysis |
title_sort | pursuing diabetic nephropathy through aqueous humor proteomics analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9537621/ https://www.ncbi.nlm.nih.gov/pubmed/36211816 http://dx.doi.org/10.1155/2022/5945828 |
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