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HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley

Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx...

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Detalles Bibliográficos
Autores principales: Feng, Xiuqin, Rahman, Md. Mostafijur, Hu, Qian, Wang, Bang, Karim, Hassan, Guzmán, Carlos, Harwood, Wendy, Xu, Qiang, Zhang, Yazhou, Tang, Huaping, Jiang, Yunfeng, Qi, Pengfei, Deng, Mei, Ma, Jian, Lan, Jingyu, Wang, Jirui, Chen, Guoyue, Lan, Xiujin, Wei, Yuming, Zheng, Youliang, Jiang, Qiantao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538149/
https://www.ncbi.nlm.nih.gov/pubmed/36212333
http://dx.doi.org/10.3389/fpls.2022.1003333
Descripción
Sumario:Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx-1 in the mutant (M2-1105). The G1086A mutation is located at the 3′ splicing receptor (AG) site of the fourth intron, resulting in an abnormal RNA splicing. The A2424G mutation was a synonymous mutation in the ninth intron. The pre-mRNA of Wx-1 was incorrectly spliced and transcribed into two abnormal transcripts. The type I transcript had a 6 bp deletion in the 5′ of fifth exon, leading to a translated HvGBSSI protein lacking two amino acids with a decreased starch-binding capacity. In the type II transcript, the fourth intron was incorrectly cleaved and retained, resulting in the premature termination of the barley Wx-1 gene. The mutations in the Wx-1 decreased the enzymatic activity of the HvGBSSI enzyme and resulted in a decreased level in amylose content. This work sheds light on a new Wx-1 gene inaction mechanism.