Cargando…
HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley
Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538149/ https://www.ncbi.nlm.nih.gov/pubmed/36212333 http://dx.doi.org/10.3389/fpls.2022.1003333 |
_version_ | 1784803319611392000 |
---|---|
author | Feng, Xiuqin Rahman, Md. Mostafijur Hu, Qian Wang, Bang Karim, Hassan Guzmán, Carlos Harwood, Wendy Xu, Qiang Zhang, Yazhou Tang, Huaping Jiang, Yunfeng Qi, Pengfei Deng, Mei Ma, Jian Lan, Jingyu Wang, Jirui Chen, Guoyue Lan, Xiujin Wei, Yuming Zheng, Youliang Jiang, Qiantao |
author_facet | Feng, Xiuqin Rahman, Md. Mostafijur Hu, Qian Wang, Bang Karim, Hassan Guzmán, Carlos Harwood, Wendy Xu, Qiang Zhang, Yazhou Tang, Huaping Jiang, Yunfeng Qi, Pengfei Deng, Mei Ma, Jian Lan, Jingyu Wang, Jirui Chen, Guoyue Lan, Xiujin Wei, Yuming Zheng, Youliang Jiang, Qiantao |
author_sort | Feng, Xiuqin |
collection | PubMed |
description | Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx-1 in the mutant (M2-1105). The G1086A mutation is located at the 3′ splicing receptor (AG) site of the fourth intron, resulting in an abnormal RNA splicing. The A2424G mutation was a synonymous mutation in the ninth intron. The pre-mRNA of Wx-1 was incorrectly spliced and transcribed into two abnormal transcripts. The type I transcript had a 6 bp deletion in the 5′ of fifth exon, leading to a translated HvGBSSI protein lacking two amino acids with a decreased starch-binding capacity. In the type II transcript, the fourth intron was incorrectly cleaved and retained, resulting in the premature termination of the barley Wx-1 gene. The mutations in the Wx-1 decreased the enzymatic activity of the HvGBSSI enzyme and resulted in a decreased level in amylose content. This work sheds light on a new Wx-1 gene inaction mechanism. |
format | Online Article Text |
id | pubmed-9538149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95381492022-10-08 HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley Feng, Xiuqin Rahman, Md. Mostafijur Hu, Qian Wang, Bang Karim, Hassan Guzmán, Carlos Harwood, Wendy Xu, Qiang Zhang, Yazhou Tang, Huaping Jiang, Yunfeng Qi, Pengfei Deng, Mei Ma, Jian Lan, Jingyu Wang, Jirui Chen, Guoyue Lan, Xiujin Wei, Yuming Zheng, Youliang Jiang, Qiantao Front Plant Sci Plant Science Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx-1 in the mutant (M2-1105). The G1086A mutation is located at the 3′ splicing receptor (AG) site of the fourth intron, resulting in an abnormal RNA splicing. The A2424G mutation was a synonymous mutation in the ninth intron. The pre-mRNA of Wx-1 was incorrectly spliced and transcribed into two abnormal transcripts. The type I transcript had a 6 bp deletion in the 5′ of fifth exon, leading to a translated HvGBSSI protein lacking two amino acids with a decreased starch-binding capacity. In the type II transcript, the fourth intron was incorrectly cleaved and retained, resulting in the premature termination of the barley Wx-1 gene. The mutations in the Wx-1 decreased the enzymatic activity of the HvGBSSI enzyme and resulted in a decreased level in amylose content. This work sheds light on a new Wx-1 gene inaction mechanism. Frontiers Media S.A. 2022-09-23 /pmc/articles/PMC9538149/ /pubmed/36212333 http://dx.doi.org/10.3389/fpls.2022.1003333 Text en Copyright © 2022 Feng, Rahman, Hu, Wang, Karim, Guzmán, Harwood, Xu, Zhang, Tang, Jiang, Qi, Deng, Ma, Lan, Wang, Chen, Lan, Wei, Zheng and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Feng, Xiuqin Rahman, Md. Mostafijur Hu, Qian Wang, Bang Karim, Hassan Guzmán, Carlos Harwood, Wendy Xu, Qiang Zhang, Yazhou Tang, Huaping Jiang, Yunfeng Qi, Pengfei Deng, Mei Ma, Jian Lan, Jingyu Wang, Jirui Chen, Guoyue Lan, Xiujin Wei, Yuming Zheng, Youliang Jiang, Qiantao HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley |
title | HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley |
title_full | HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley |
title_fullStr | HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley |
title_full_unstemmed | HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley |
title_short | HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley |
title_sort | hvgbssi mutation at the splicing receptor site affected rna splicing and decreased amylose content in barley |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538149/ https://www.ncbi.nlm.nih.gov/pubmed/36212333 http://dx.doi.org/10.3389/fpls.2022.1003333 |
work_keys_str_mv | AT fengxiuqin hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT rahmanmdmostafijur hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT huqian hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT wangbang hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT karimhassan hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT guzmancarlos hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT harwoodwendy hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT xuqiang hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT zhangyazhou hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT tanghuaping hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT jiangyunfeng hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT qipengfei hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT dengmei hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT majian hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT lanjingyu hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT wangjirui hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT chenguoyue hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT lanxiujin hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT weiyuming hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT zhengyouliang hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley AT jiangqiantao hvgbssimutationatthesplicingreceptorsiteaffectedrnasplicinganddecreasedamylosecontentinbarley |