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Control of actin polymerization via reactive oxygen species generation using light or radiation
Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility, and morphology. It is known that actin and ot...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538341/ https://www.ncbi.nlm.nih.gov/pubmed/36211457 http://dx.doi.org/10.3389/fcell.2022.1014008 |
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author | Ishimoto, Tetsuya Mori, Hisashi |
author_facet | Ishimoto, Tetsuya Mori, Hisashi |
author_sort | Ishimoto, Tetsuya |
collection | PubMed |
description | Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility, and morphology. It is known that actin and other actin-binding proteins are targets for reactive oxygen species (ROS), indicating that ROS affects cells through actin reorganization. Several researchers have attempted to control actin polymerization from outside the cell to mimic or inhibit actin reorganization. To modify the polymerization state of actin, ultraviolet, visible, and near-infrared light, ionizing radiation, and chromophore-assisted light inactivation have all been reported to induce ROS. Additionally, a combination of the fluorescent protein KillerRed and the luminescent protein luciferase can generate ROS on actin fibers and promote actin polymerization. These techniques are very useful tools for analyzing the relationship between ROS and cell function, movement, and morphology, and are also expected to be used in therapeutics. In this mini review, we offer an overview of the advancements in this field, with a particular focus on how to control intracellular actin polymerization using such optical approaches, and discuss future challenges. |
format | Online Article Text |
id | pubmed-9538341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95383412022-10-08 Control of actin polymerization via reactive oxygen species generation using light or radiation Ishimoto, Tetsuya Mori, Hisashi Front Cell Dev Biol Cell and Developmental Biology Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility, and morphology. It is known that actin and other actin-binding proteins are targets for reactive oxygen species (ROS), indicating that ROS affects cells through actin reorganization. Several researchers have attempted to control actin polymerization from outside the cell to mimic or inhibit actin reorganization. To modify the polymerization state of actin, ultraviolet, visible, and near-infrared light, ionizing radiation, and chromophore-assisted light inactivation have all been reported to induce ROS. Additionally, a combination of the fluorescent protein KillerRed and the luminescent protein luciferase can generate ROS on actin fibers and promote actin polymerization. These techniques are very useful tools for analyzing the relationship between ROS and cell function, movement, and morphology, and are also expected to be used in therapeutics. In this mini review, we offer an overview of the advancements in this field, with a particular focus on how to control intracellular actin polymerization using such optical approaches, and discuss future challenges. Frontiers Media S.A. 2022-09-23 /pmc/articles/PMC9538341/ /pubmed/36211457 http://dx.doi.org/10.3389/fcell.2022.1014008 Text en Copyright © 2022 Ishimoto and Mori. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Ishimoto, Tetsuya Mori, Hisashi Control of actin polymerization via reactive oxygen species generation using light or radiation |
title | Control of actin polymerization via reactive oxygen species generation using light or radiation |
title_full | Control of actin polymerization via reactive oxygen species generation using light or radiation |
title_fullStr | Control of actin polymerization via reactive oxygen species generation using light or radiation |
title_full_unstemmed | Control of actin polymerization via reactive oxygen species generation using light or radiation |
title_short | Control of actin polymerization via reactive oxygen species generation using light or radiation |
title_sort | control of actin polymerization via reactive oxygen species generation using light or radiation |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538341/ https://www.ncbi.nlm.nih.gov/pubmed/36211457 http://dx.doi.org/10.3389/fcell.2022.1014008 |
work_keys_str_mv | AT ishimototetsuya controlofactinpolymerizationviareactiveoxygenspeciesgenerationusinglightorradiation AT morihisashi controlofactinpolymerizationviareactiveoxygenspeciesgenerationusinglightorradiation |