A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care

Here, a novel biosensing platform for the detection of SARS‐CoV‐2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi‐walled carbon nanotubes (MWCNTs) screen‐printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS‐CoV‐...

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Detalles Bibliográficos
Autores principales: Tortolini, Cristina, Angeloni, Antonio, Antiochia, Riccarda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538619/
https://www.ncbi.nlm.nih.gov/pubmed/36247366
http://dx.doi.org/10.1002/elan.202200349
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author Tortolini, Cristina
Angeloni, Antonio
Antiochia, Riccarda
author_facet Tortolini, Cristina
Angeloni, Antonio
Antiochia, Riccarda
author_sort Tortolini, Cristina
collection PubMed
description Here, a novel biosensing platform for the detection of SARS‐CoV‐2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi‐walled carbon nanotubes (MWCNTs) screen‐printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS‐CoV‐2 spike protein (SP), a bioactive layer of chitosan (CS) and protein A (PrA). The voltammetric sensor showed superior performances both in phosphate buffer solution (PBS) and spiked‐saliva samples, with LOD values of 5.0±0.1 and 30±2.1 ng/mL, compared to 20±1.8 and 50±2.5 ng/mL for the impedimetric sensor. Moreover, the voltammetric immunosensor was tested in real saliva, showing promising results.
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spelling pubmed-95386192022-10-11 A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care Tortolini, Cristina Angeloni, Antonio Antiochia, Riccarda Electroanalysis Research Articles Here, a novel biosensing platform for the detection of SARS‐CoV‐2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi‐walled carbon nanotubes (MWCNTs) screen‐printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS‐CoV‐2 spike protein (SP), a bioactive layer of chitosan (CS) and protein A (PrA). The voltammetric sensor showed superior performances both in phosphate buffer solution (PBS) and spiked‐saliva samples, with LOD values of 5.0±0.1 and 30±2.1 ng/mL, compared to 20±1.8 and 50±2.5 ng/mL for the impedimetric sensor. Moreover, the voltammetric immunosensor was tested in real saliva, showing promising results. John Wiley and Sons Inc. 2022-09-29 /pmc/articles/PMC9538619/ /pubmed/36247366 http://dx.doi.org/10.1002/elan.202200349 Text en © 2022 The Authors. Electroanalysis published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Tortolini, Cristina
Angeloni, Antonio
Antiochia, Riccarda
A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care
title A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care
title_full A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care
title_fullStr A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care
title_full_unstemmed A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care
title_short A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS‐CoV‐2 Detection at the Point‐of‐care
title_sort comparative study of voltammetric vs impedimetric immunosensor for rapid sars‐cov‐2 detection at the point‐of‐care
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538619/
https://www.ncbi.nlm.nih.gov/pubmed/36247366
http://dx.doi.org/10.1002/elan.202200349
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