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Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors

Severe Acute Respiratory Syndrome Coronavirus‐2 (SARS‐CoV‐2) is the novel coronavirus responsible for worldwide coronavirus disease (COVID‐19). We previously observed that Angiotensin‐converting enzyme 2 (ACE2) and Dipeptidyl peptidase‐4 (DPP4) are significantly overexpressed in naso‐oropharyngeal s...

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Autores principales: Latini, Andrea, Vancheri, Chiara, Amati, Francesca, Morini, Elena, Grelli, Sandro, Claudia, Matteucci, Vita, Petrone, Colona, Vito Luigi, Murdocca, Michela, Andreoni, Massimo, Malagnino, Vincenzo, Raponi, Massimiliano, Cocciadiferro, Dario, Novelli, Antonio, Borgiani, Paola, Novelli, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538662/
https://www.ncbi.nlm.nih.gov/pubmed/36073344
http://dx.doi.org/10.1111/jcmm.17492
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author Latini, Andrea
Vancheri, Chiara
Amati, Francesca
Morini, Elena
Grelli, Sandro
Claudia, Matteucci
Vita, Petrone
Colona, Vito Luigi
Murdocca, Michela
Andreoni, Massimo
Malagnino, Vincenzo
Raponi, Massimiliano
Cocciadiferro, Dario
Novelli, Antonio
Borgiani, Paola
Novelli, Giuseppe
author_facet Latini, Andrea
Vancheri, Chiara
Amati, Francesca
Morini, Elena
Grelli, Sandro
Claudia, Matteucci
Vita, Petrone
Colona, Vito Luigi
Murdocca, Michela
Andreoni, Massimo
Malagnino, Vincenzo
Raponi, Massimiliano
Cocciadiferro, Dario
Novelli, Antonio
Borgiani, Paola
Novelli, Giuseppe
author_sort Latini, Andrea
collection PubMed
description Severe Acute Respiratory Syndrome Coronavirus‐2 (SARS‐CoV‐2) is the novel coronavirus responsible for worldwide coronavirus disease (COVID‐19). We previously observed that Angiotensin‐converting enzyme 2 (ACE2) and Dipeptidyl peptidase‐4 (DPP4) are significantly overexpressed in naso‐oropharyngeal swabs (NPS) of COVID‐19 patients, suggesting their putative functional role in the disease progression. ACE2 and DPP4 overexpression in COVID‐19 patients may be associated to epigenetic mechanism, such as miRNA differential expression. We investigated if hsa‐let7b‐5p, reported to target both ACE2 and DPP4 transcripts, could be involved in the regulation of these genes. We verified that the inhibition and overexpression of hsa‐let7b‐5p matched to a modulation of both ACE2 and DPP4 levels. Then, we observed a statistically significant downregulation (FC = −1.5; p < 0.05) of hsa‐let7b‐5p in the same COVID‐19 and control samples of our previous study. This is the first study that shows hsa‐let7b‐5p low expression in naso‐oropharyngeal swabs of COVID‐19 patients and demonstrates a functional role of this miR in regulating ACE2 and DPP4 levels. These data suggest the involvement of hsa‐let7b‐5p in the regulation of genes necessary for SARS‐CoV‐2 infections and its putative role as a therapeutic target for COVID‐19.
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spelling pubmed-95386622022-10-11 Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors Latini, Andrea Vancheri, Chiara Amati, Francesca Morini, Elena Grelli, Sandro Claudia, Matteucci Vita, Petrone Colona, Vito Luigi Murdocca, Michela Andreoni, Massimo Malagnino, Vincenzo Raponi, Massimiliano Cocciadiferro, Dario Novelli, Antonio Borgiani, Paola Novelli, Giuseppe J Cell Mol Med Original Articles Severe Acute Respiratory Syndrome Coronavirus‐2 (SARS‐CoV‐2) is the novel coronavirus responsible for worldwide coronavirus disease (COVID‐19). We previously observed that Angiotensin‐converting enzyme 2 (ACE2) and Dipeptidyl peptidase‐4 (DPP4) are significantly overexpressed in naso‐oropharyngeal swabs (NPS) of COVID‐19 patients, suggesting their putative functional role in the disease progression. ACE2 and DPP4 overexpression in COVID‐19 patients may be associated to epigenetic mechanism, such as miRNA differential expression. We investigated if hsa‐let7b‐5p, reported to target both ACE2 and DPP4 transcripts, could be involved in the regulation of these genes. We verified that the inhibition and overexpression of hsa‐let7b‐5p matched to a modulation of both ACE2 and DPP4 levels. Then, we observed a statistically significant downregulation (FC = −1.5; p < 0.05) of hsa‐let7b‐5p in the same COVID‐19 and control samples of our previous study. This is the first study that shows hsa‐let7b‐5p low expression in naso‐oropharyngeal swabs of COVID‐19 patients and demonstrates a functional role of this miR in regulating ACE2 and DPP4 levels. These data suggest the involvement of hsa‐let7b‐5p in the regulation of genes necessary for SARS‐CoV‐2 infections and its putative role as a therapeutic target for COVID‐19. John Wiley and Sons Inc. 2022-09-08 2022-10 /pmc/articles/PMC9538662/ /pubmed/36073344 http://dx.doi.org/10.1111/jcmm.17492 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Latini, Andrea
Vancheri, Chiara
Amati, Francesca
Morini, Elena
Grelli, Sandro
Claudia, Matteucci
Vita, Petrone
Colona, Vito Luigi
Murdocca, Michela
Andreoni, Massimo
Malagnino, Vincenzo
Raponi, Massimiliano
Cocciadiferro, Dario
Novelli, Antonio
Borgiani, Paola
Novelli, Giuseppe
Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors
title Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors
title_full Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors
title_fullStr Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors
title_full_unstemmed Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors
title_short Expression analysis of miRNA hsa‐let7b‐5p in naso‐oropharyngeal swabs of COVID‐19 patients supports its role in regulating ACE2 and DPP4 receptors
title_sort expression analysis of mirna hsa‐let7b‐5p in naso‐oropharyngeal swabs of covid‐19 patients supports its role in regulating ace2 and dpp4 receptors
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9538662/
https://www.ncbi.nlm.nih.gov/pubmed/36073344
http://dx.doi.org/10.1111/jcmm.17492
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